Utilisation of phosphorus nutrient content in industrial scale plasmid DNA production: a waste minimisation study

Cliffe, Finola E.; Walsh, Gary; O’Dwyer, Thomas F.

doi:10.1016/j.jclepro.2010.02.013

Cited by 1 publication

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Media ingredients are relatively inexpensive and are often added in excess. Potential environmental consequences of unutilized media ingredients were generally given little consideration during process development (Cliffe et al 2010). Of particular environmental concern is the phosphorus (P) content of such waste media which, if not properly treated, can lead to eutrophication in water bodies, thereby leading to significantly reduced water quality (Khan and Ansari 2005).…”

Section: Introductionmentioning

confidence: 99%

“…A preliminary study has indicated that the phosphorous content of selected media used to produce a niche product (plasmid DNA) could be reduced without affecting product yield (Cliffe et al 2010). The current study investigates the potential for P minimization in the context of what may quantitatively be the most widespread global application of modern microbial biotechnology, the production of recombinant proteins in engineered E. coli.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Minimization of phosphorus in the fermentation media of Escherichia coli producing a model recombinant protein

Witt

O’Dwyer

Walsh

2014

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

In the biotechnology sector, a main processing goal is the production of high cell (and hence product) yields. Therefore, little consideration is traditionally given to the potential environmental impacts of excess culture media ingredients. This study investigated the scope for reducing the quantities of phosphorus (P) present in both a complex terrific broth (TB) and semi-defined minimal media 9/yeast extract (M9/YE) fermentation media used to culture a model Escherichia coli strain engineered to produce a recombinant b-galactosidase. Reductions of up to 70 % did not adversely affect biomass yields attained; however, further P minimization leads to a drop in dry cell weight obtained, particularly in the case of semi-defined media. P concentration in TB media had little effect upon total recombinant protein expression levels achieved. In the case of M9/YE media, reductions [70 % P negatively affected product expression levels. Protein functionality, assessed by k m and V max , was not influenced by the type of media nor the P concentration present. Overall, the results indicate that P can be reduced by a minimum of 70 % without adversely affecting the biomass yield, the recombinant protein yield or functionality. Such reductions should lead to significant P savings in the large-scale manufacturing of proteins produced by genetic engineering in E. coli.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%