Utilising Reuse and Recycling Strategies in Costume Design in Kuwait Theatres

Dashti, Ali A.

doi:10.7190/shu-thesis-00250

Cited by 1 publication

(1 citation statement)

References 42 publications

(121 reference statements)

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“…Isolated and presumed Escherichia coli and Pseudomonas colonies from nutrient agar plates were cultured overnight in 5 ml of nutrient broth within test tubes at 37ºC with aeration, utilizing a shaking water bath set at 120 rpm. After that, a 1.5 mL Eppendorf tube was filled with a 1 mL aliquot of overnight pure cultures, and the boiling method was used to extract DNA (Dashti A and Dashti H, 2009). In brief, the samples were centrifuged for 5 minutes at 14,000 rpm, the supernatant was discarded and the step was repeated.…”

Section: Detection Of Virulence Genes By Gene-specific Polymerase Cha...mentioning

confidence: 99%

Analysis of Microbiological Quality and Antibiotic Resistance Patterns in Milk Supply Chain

Rinky,

Reza,

Nowar

et al. 2023

Bioresearch Communications

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The widespread consumption of milk for its nutritional value and health benefits brings the risk of milk-borne diseases due to the presence of various microorganisms, including antibiotic-resistant pathogens. This has led to an increased focus on ensuring the safety of milk products across the supply chain by dairy industries. The study aimed to evaluate microbiological parameters and detect multi-antibiotic-resistant pathogens at three specific supply points and to explore the association between the presence of residual antibiotics and the resistant isolates in milk samples. About 50 milk samples, including raw, soon-after-processed, packaged marketed pasteurized, and UHT milk, were subjected to microbiological analysis. This involved assessing the total bacterial count (TBC) and total coliform count (TCC), conducting antibiotic susceptibility tests through disk and well diffusion assays, detecting virulence genes in multi-antibiotic resistant isolates using gene-specific PCR, and analyzing residual antibiotics by HPLC. The study revealed that the quality of raw milk samples was unacceptable (TBC >4.5x107 CFU/mL and TCC >5.6x104 CFU/mL), while pasteurized samples from processing plants had lower counts than those from retail stores (TBC >5x105 CFU/mL and TCC >1.6x104 CFU/mL) indicating post-pasteurization contamination. About 70.37% of the isolates were Gram-negative, with Escherichia coli (21.4%) and Vibrio (18.8%) being the most prevalent. Resistance to antibiotics was substantial, particularly against ampicillin (86.3%), tetracycline (76%), and ciprofloxacin (58.9%). Gene-specific PCR analysis detected uidA, oprL, and oprI virulence genes in multi-drug-resistant Escherichia coli and Pseudomonas sp. respectively. The study also revealed a direct association between the presence of residual antibiotics and the resistant isolates, emphasizing the need for dairy industry improvements. As high bacterial counts in milk can pose health risks by fostering antibiotic-resistant pathogens, it is essential to mitigate microbiological contamination in the milk supply chain through the implementation of various precautionary measures. Bioresearch Commu. 10(1): 1462-1473, 2024 (January)

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Section: Detection Of Virulence Genes By Gene-specific Polymerase Cha...mentioning

confidence: 99%