Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery

Levin, Brett; Redmond, Sharon L.; Rajkhowa, Rangam; Eikelboom, Robert H.; Atlas, Marcus D.; Marano, Robert J.

doi:10.1017/s0022215112001661

Cited by 35 publications

(23 citation statements)

References 34 publications

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“…Following the removal of sericin, an antigenic glue‐like coating protein, the core silk fibroin fibers are biocompatible with little immune response postimplantation . Previous studies demonstrated that silk fibroin could support cell adhesion, proliferation, and differentiation in vitro, including human TM keratinocytes . We recently reported hearing and healing benefits of silk fibroin membranes using onlay myringoplasty in rats, with improved structural outcomes .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown its efficacy to promote wound healing of skin, ligament, nerves, and bone . Our group has demonstrated that silk fibroin can successfully support human TM cells in vitro . Collagen has been widely used as a bioscaffold due to its biocompatibility and ability to promote the growth of many types of cells and tissues .…”

Section: Introductionmentioning

confidence: 96%

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Tympanic membrane repair using silk fibroin and acellular collagen scaffolds

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Tympanic membrane repair using silk fibroin and acellular collagen scaffolds

et al. 2013

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“…SF is rich in β‐sheet structures owing to hydrophobic domains which provide a good resistance to water solubility, and lead to the high mechanical properties of these materials . SF can be processed into fibers, membranes, films, meshes, foams, and hydrogels for the repair/regeneration of several tissues.…”

Section: Natural Polymers and Calcium Phosphate Materials Propertiesmentioning

confidence: 99%

Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review

2015

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Tissue engineering and regenerative medicine has been providing exciting technologies for the development of functional substitutes aimed to repair and regenerate damaged tissues and organs. Inspired by the hierarchical nature of bone, nanostructured biomaterials are gaining a singular attention for tissue engineering, owing their ability to promote cell adhesion and proliferation, and hence new bone growth, compared with conventional microsized materials. Of particular interest are nanocomposites involving biopolymeric matrices and bioactive nanosized fillers. Biodegradability, high mechanical strength, and osteointegration and formation of ligamentous tissue are properties required for such materials. Biopolymers are advantageous due to their similarities with extracellular matrices, specific degradation rates, and good biological performance. By its turn, calcium phosphates possess favorable osteoconductivity, resorbability, and biocompatibility. Herein, an overview on the available natural polymer/calcium phosphate nanocomposite materials, their design, and properties is presented. Scaffolds, hydrogels, and fibers as biomimetic strategies for tissue engineering, and processing methodologies are described. The specific biological properties of the nanocomposites, as well as their interaction with cells, including the use of bioactive molecules, are highlighted. Nanocomposites in vivo studies using animal models are also reviewed and discussed.

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“…Silk fibroin materials have effective mechanical properties for TM tissue engineering (21) and provide a suitable substrate for human TM keratinocytes (18). In order to test whether these TM cells would support a tissue-engineering approach, we cultured explant-derived cells on porous silk membrane scaffolds (Supplementary Figure S3A).…”

Section: Resultsmentioning

confidence: 99%

Tympanic Membrane Organ Culture using Cell Culture well Inserts Engrafted with Tympanic Membrane Tissue Explants

2017

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Tissue engineering approaches using growth factors and various materials for repairing chronic perforations of the tympanic membrane are being developed, but there are surprisingly few relevant tissue culture models available to test new treatments. Here, we present a simple three-dimensional model system based on micro-dissecting the rat tympanic membrane umbo and grafting it into the membrane of a cell culture well insert. Cell outgrowth from the graft produced sufficient cells to populate a membrane of similar surface area to the human tympanic membrane within 2 weeks. Tissue grafts from the annulus region also showed cell outgrowth but were not as productive. The umbo organoid supported substantial cell proliferation and migration under the influence of keratinocyte growth medium. Cells from umbo grafts were enzymatically harvested from the polyethylene terephthalate (PET) membrane for expansion in routine culture and cells could be harvested consecutively from the same graft over multiple cycles. We used harvested cells to test cell migration properties and to engraft a porous silk scaffold material as proof-of-principle for tissue engineering applications. This model is simple enough to be widely adopted for tympanic membrane regeneration studies and has promise as a tissue-equivalent model alternative to animal testing.

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Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery

Abstract: The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty.

Cited by 35 publications

References 34 publications

Tympanic membrane repair using silk fibroin and acellular collagen scaffolds

Tympanic membrane repair using silk fibroin and acellular collagen scaffolds

Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review

Tympanic Membrane Organ Culture using Cell Culture well Inserts Engrafted with Tympanic Membrane Tissue Explants

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