Utility of a xanthene‐based dye for determination of nilotinib using two spectroscopic approaches. Applications to bulk powder, capsules, and spiked human plasma

Abstract

Novel, selective, facile, and precise spectroscopic approaches were validated to determine nilotinib hydrochloride, a tyrosine kinase inhibitor used to treat patients with chronic myeloid leukemia. These approaches depend on the reaction of the tertiary amine group of nilotinib with erythrosine B in the Britton-Robinson buffer at pH 4.Method I, depends on measuring the absorbance of the formed complex at 551 nm.The absorbance concentration plot showed linearity over the concentration range of 1.0 to 9.0 μg/ml. Method II, involved the measurement of the quenching of the native fluorescence of erythrosine B by adding nilotinib in an acidic medium. The fluorescence quenching of erythrosine B was measured at 549 nm after excitation at 528 nm. This approach showed excellent linearity in the concentration range of 0.04 to 0.7 μg/ml. The limit of detection values for Method I and Method II were 0.225 and 0.008 μg/ml, respectively, while the limit of quantitation values for Method I and Method II were 0.68 and 0.026 μg/ml, respectively. To get the optimal conditions, factors that may affect the formation of the ion-pairing complex were thoroughly examined. The two approaches were carefully validated following the International Conference of Harmonization (ICH Q2R1) guidelines. Statistical assessment of the results achieved using the suggested and previously published comparison approaches showed no significant difference. The approaches were successful in determining nilotinib in a pharmaceutical dosage form as well as spiked human plasma samples. The eco-friendly properties of the methods were evaluated by three different tools.

A green, fluorescent probe employing erythrosine-B for tracing the accidental administration of levamisole in milk and plasma samples

“…The signicance of spectrouorimetry for the determination of various pharmaceuticals in different matrices could be illustrated by reviewing early reported methods in the literature. [17][18][19][20][21][22][23][24][25][26] The present work aims to develop and validate a simple uorimetric assay of LVM in milk and plasma samples to analyze its accidental consumption, to protect consumers and/ or patients undergoing treatment for cocaine addiction and receiving LVM adulterated doses from the lethal side effects of the concerned analyte.…”

Validated spectrofluorimetric and resonance Rayleigh scattering methods for determining naftidrofuryl in varied pharmaceutical samples based on its interaction with erythrosin B

“…Recently, xanthene dyes (eosin Y, erythrosin B, fluorescein, merbromin) have been used as colorimetric, fluorescent, and/or RRS analytical reagents for determining a wide range of active pharmaceutical ingredients [12]. For example, eosin Y and merbromin dyes have been reported for determining daclatasvir, cyclobenzaprine, fluvoxamine, atomoxetine, oxybutynin, dexlansoprazole and some β-blockers [13][14][15][16][17][18][19], while erythrosin B has been utilized for determining rupatadine, duloxetine, terbinafine, venlafaxine, nilotinib trospium, and varenicline [20][21][22][23][24][25][26]. The benefits of using erythrosin B include its low price and a high degree of stability.…”

Utility of the food colorant erythrosine B as an effective green probe for quantitation of the anticancer sunitinib. Application to pharmaceutical formulations and human plasma

“…The utilization of xanthene dyes as fluorometric reagents has been reported in the analysis of various pharmaceutical compounds [25][26][27][28][29][30][31][32][33][34]. The goal of this research is to study the interaction of EB with SUN.…”