Utility of Animal Models for Human Embryo Culture: Nonhuman Primates

Simerly, Calvin; Schatten, Gerald

doi:10.1007/978-1-61779-971-6_4

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…It is our experience and that of others that some non-human primate embryos will become expanded blastocysts and hatch by day 7–8 23 24 43 . Nevertheless, there are no studies, including this one, that compare the potential of early or late expanding blastocyst embryos in rhesus macaque to embryo quality such as ploidy or ability to implant.…”

Section: Discussionmentioning

confidence: 89%

Abnormal Early Cleavage Events Predict Early Embryo Demise: Sperm Oxidative Stress and Early Abnormal Cleavage

Burruel

Klooster

Barker

et al. 2014

Sci Rep

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Human embryos resulting from abnormal early cleavage can result in aneuploidy and failure to develop normally to the blastocyst stage. The nature of paternal influence on early embryo development has not been directly demonstrated although many studies have suggested effects from spermatozoal chromatin packaging, DNA damage, centriolar and mitotic spindle integrity, and plasma membrane integrity. The goal of this study was to determine whether early developmental events were affected by oxidative damage to the fertilizing sperm. Survival analysis was used to compare patterns of blastocyst formation based on P2 duration. Kaplan-Meier survival curves demonstrate that relatively few embryos with short (<1 hr) P2 times reached blastocysts, and the two curves diverged beginning on day 4, with nearly all of the embryos with longer P2 times reaching blastocysts by day 6 (p < .01). We determined that duration of the 2nd to 3rd mitoses were sensitive periods in the presence of spermatozoal oxidative stress. Embryos that displayed either too long or too short cytokineses demonstrated an increased failure to reach blastocyst stage and therefore survive for further development. Although paternal-derived gene expression occurs later in development, this study suggests a specific role in early mitosis that is highly influenced by paternal factors.

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Section: Discussionmentioning

confidence: 89%

Abnormal Early Cleavage Events Predict Early Embryo Demise: Sperm Oxidative Stress and Early Abnormal Cleavage

Burruel

Klooster

Barker

et al. 2014

Sci Rep

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“…Historically this has been most strongly recognized in pharmacokinetic studies [3, 4]. More recently, species differences have been pushed further with nonhuman primates showing increased translational validity in, for instance, regenerative medicine utilizing induced pluripotent stem cells [5, 6] and antiviral antibody therapy development [7, 8]. Macaque species are the most common nonhuman primate model, including the rhesus macaque ( Macaca mulatta ) and the long-tailed macaque ( M. fascicularis ), also commonly known as the crab-eating or cynomolgus macaque.…”

Section: Introductionmentioning

confidence: 99%

Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

Ogawa

Vallender

2014

BMC Genomics

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BackgroundNonhuman primates are commonly used in biomedical research as animal models of human disease and behavior. Compared to common rodent models, nonhuman primates are genetically, physiologically, behaviorally and neurologically more similar to humans owing to more recent shared ancestry and therefore provide the advantage of greater translational validity in preclinical studies. The cynomolgus macaque (Macaca fascicularis) is one of the most commonly used nonhuman primates in academic and industry settings, yet population genetic research has revealed significant substructure throughout the species distribution that may confound studies. Cynomolgus monkeys introduced to Mauritius specifically have previously been thought to maintain the least genetic heterogeneity of all cynomolgus monkeys, although recent work, including work from our lab, suggests macaques from Mauritius too may harbor cryptic substructure.ResultsTo evaluate putative substructure in Mauritian cynomolgus macaques, we designed a panel of 96 single nucleotide polymorphisms based on preliminary findings from previous work to screen 246 of cynomolgus monkeys from two primary suppliers. Results from this study support substructure in Mauritian macaques and suggest a minimum of two populations and maybe three on Mauritius, with moderate admixture.ConclusionThese findings inform the natural history of these monkeys suggesting either a previously unrecognized physical or ecological barrier to gene flow on Mauritius and/or the breakdown of historic substructure resulting from the history of macaque introduction to the island. These findings are relevant to ongoing research using these models in part because of increased appreciation of segregating common variation with functional effects and may be used to better inform animal selection in preclinical research.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-748) contains supplementary material, which is available to authorized users.

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In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Ramsey

Hanna

2019

Methods in Molecular Biology

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Utility of Animal Models for Human Embryo Culture: Nonhuman Primates

Cited by 4 publications

References 51 publications

Abnormal Early Cleavage Events Predict Early Embryo Demise: Sperm Oxidative Stress and Early Abnormal Cleavage

Abnormal Early Cleavage Events Predict Early Embryo Demise: Sperm Oxidative Stress and Early Abnormal Cleavage

Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

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