Utility of CHROMagar™ Candida Plus for presumptive identification of Candida auris from surveillance samples

Marathe, Anuradha; Zhu, YanChun; Chaturvedi, Sudha; Chaturvedi, Sudha

doi:10.1007/s11046-022-00656-3

Cited by 13 publications

(10 citation statements)

References 26 publications

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“…In this sense, other authors also reported the misidentification of C. pseudohaemulonii and C. vulturna strains as C. auris 6,25 . Moreover, strains belonging to unrelated species have also been misidentified as C. auris , including several strains of the C. parapsilosis complex, which has also been reported by other authors 10,19 . Therefore, the presumptive identification of C. auris by using the CCP medium should be confirmed by other more specific methods, like MALDI‐TOF MS or DNA sequencing.…”

Section: Discussionmentioning

confidence: 66%

“…6,25 Moreover, strains belonging to unrelated species have also been misidentified as C. auris, including several strains of the C. parapsilosis complex, which has also been reported by other authors. 10,19 MALDI-TOF MS identification by using the CCP medium had a similar performance than identification by using the SDA medium, 94.0% vs. 94.3% of correct identifications, respectively. 23 However, the statistical analysis showed that, although there is no difference in the number of correctly identified strains (score >1.700) as mentioned above, the MALDI-TOF MS score values using the CCP medium are lower than using the SDA medium.…”

Section: Discussionmentioning

confidence: 82%

“…The CHROMagar Candida Plus is a new chromogenic medium suitable for the isolation and presumptive identification of C. albicans , C. tropicalis , P. kudriavzevii , C. glabrata and C. auris 12 . Some studies have already evaluated this medium showing that it is useful to detect C. auris in surveillance and environmental samples 2,10–12 . However, our study is a challenging evaluation of the CCP medium to detect possible misidentifications.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the CHROMagar Candida Plus medium for presumptive identification of yeasts and MALDI‐TOF MS identification

Taverna

Vivot

Arias

et al. 2023

Mycoses

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Rapid and accurate yeasts species identification in clinical laboratories is important for appropriate and timely antifungal treatment. We evaluate the performance of the new medium CHROMagar™ Candida Plus for presumptive identification of yeasts species and MALDI‐TOF identification. We identify 303 strains belonging to 60 clinically relevant yeasts species by using the new medium. Presumptive identification was correct at the Candida albicans complex, Candida tropicalis and Pichia kudriavzevii (Candida krusei) species. However, although this medium was able to identify all Candida auris and Candida glabrata strains, other species were misidentified as C. auris or C. glabrata. A total of 215 strains were identified by using MALDI‐TOF and evaluated two incubation temperatures (30°C and 37°C) and two incubation times (24 h and 72 h). Most strains (94%; 202/215) were correctly identified at the species (n:190) or complex level (n:12) at both temperatures and incubation times. However, we observed that the time of incubation (24 h vs. 72 h) affects the score values when yeasts are incubated at 37°C, but does not affect score values when yeasts are incubated at 30°C. In conclusion, the new medium has a good performance in the presumptive identification of the C. albicans complex, C. tropicalis and P. kudriavzevii (C. krusei). In addition, this medium is useful for the screening of C. auris and C. glabrata isolates, but identification should be confirmed by other more specific techniques, like MALDI‐TOF.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the CHROMagar Candida Plus medium for presumptive identification of yeasts and MALDI‐TOF MS identification

Taverna

Vivot

Arias

et al. 2023

Mycoses

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“…CHROMagar was used for the isolation and differentiation of Candida species. The C. albicans appeared green color colonies ( 13 ). All seventy strains were cultured on CHROMagar, among them, 50 strains were created green colonies as Candida albicans.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans

Habibi,

Bayat,

Omidi

et al. 2023

IJM

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Background and Objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains. Materials and Methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chlo- ride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry. Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells. Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design.

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“…An excellent alternative to standard fungal media for screening patients potentially colonised/infected with C. auris is CHROMagarTM Candida Plus. At 36 h of incubation, it has a sensitivity and specificity of 100%, with C. auris colonies appearing as a light blue colour with a blue halo [82]. Options for species identification include matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and molecular methods (Figure 4) [80].…”

Section: Identification and Typingmentioning

confidence: 99%

Candida auris Outbreaks: Current Status and Future Perspectives

De Gaetano,

Midiri,

Mancuso

et al. 2024

Microorganisms

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Candida auris has been identified by the World Health Organization (WHO) as a critical priority pathogen on its latest list of fungi. C. auris infections are reported in the bloodstream and less commonly in the cerebrospinal fluid and abdomen, with mortality rates that range between 30% and 72%. However, no large-scale epidemiology studies have been reported until now. The diagnosis of C. auris infections can be challenging, particularly when employing conventional techniques. This can impede the early detection of outbreaks and the implementation of appropriate control measures. The yeast can easily spread between patients and in healthcare settings through contaminated environments or equipment, where it can survive for extended periods. Therefore, it would be desirable to screen patients for C. auris colonisation. This would allow facilities to identify patients with the disease and take appropriate prevention and control measures. It is frequently unsusceptible to drugs, with varying patterns of resistance observed among clades and geographical regions. This review provides updates on C. auris, including epidemiology, clinical characteristics, genomic analysis, evolution, colonisation, infection, identification, resistance profiles, therapeutic options, prevention, and control.

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Utility of CHROMagar™ Candida Plus for presumptive identification of Candida auris from surveillance samples

Cited by 13 publications

References 26 publications

Evaluation of the CHROMagar Candida Plus medium for presumptive identification of yeasts and MALDI‐TOF MS identification

Evaluation of the CHROMagar Candida Plus medium for presumptive identification of yeasts and MALDI‐TOF MS identification

Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans

Candida auris Outbreaks: Current Status and Future Perspectives

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