Utility of clinical metagenomics in diagnosing malignancies in a cohort of patients with Epstein-Barr virus positivity

Song, Jieyu; Zhu, Kun; Wang, Xiaojia; Yang, Qingluan; Yu, Shenglei; Zhang, Yi; Fu, Zhangfan; Wang, Hongyu; Zhao, Yuanhan; Lin, Ke; Yuan, Guanmin; Guo, Jingxin; Shi, Yingqi; Liu, Chao; Ai, Jingwen; Zhang, Haocheng; Zhang, Wenhong

doi:10.3389/fcimb.2023.1211732

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…New studies have extended its application in identifying tumors in body fluids and cerebrospinal fluids. Therefore, mNGS could be a useful tool to rapidly detect both pathogens and neoplasms in children with FUO [75]. The early detection of mNGS can shorten both the time to reach diagnosis and the days of hospitalization and reduce unnecessary antibiotic consumption [76].…”

Section: Future Perspectivesmentioning

confidence: 99%

Update on Fever of Unknown Origin in Children: Focus on Etiologies and Clinical Approach

Trapani,

Fiordelisi,

Stinco

et al. 2023

Children

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Fever of unknown origin (FUO) can be caused by four etiological categories of diseases. The most common cause of FUO in children is represented by infections, followed by inflammatory conditions and neoplastic causes; a decreasing quote remains still without diagnosis. Despite the fact that several diagnostic and therapeutic approaches have been proposed since the first definition of FUO, none of them has been fully validated in pediatric populations. A focused review of the patient’s history and a thorough physical examination may offer helpful hints in suggesting a likely diagnosis. The diagnostic algorithm should proceed sequentially, and invasive testing should be performed only in select cases, possibly targeted by a diagnostic suspect. Pioneering serum biomarkers have been developed and validated; however, they are still far from becoming part of routine clinical practice. Novel noninvasive imaging techniques have shown promising diagnostic accuracy; however, their positioning in the diagnostic algorithm of pediatric FUO is still not clear. This narrative review aims to provide a synopsis of the existent literature on FUO in children, with its major causes and possible diagnostic workup, to help the clinician tackle the complex spectrum of pediatric FUO in everyday clinical practice.

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Section: Future Perspectivesmentioning

confidence: 99%

Update on Fever of Unknown Origin in Children: Focus on Etiologies and Clinical Approach

Trapani,

Fiordelisi,

Stinco

et al. 2023

Children

View full text Add to dashboard Cite

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Diagnostic performance of metagenomic next-generation sequencing among hematological malignancy patients with bloodstream infections after antimicrobial therapy

Xu,

Peng,

Zhou

et al. 2024

Journal of Infection

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Active herpesviruses are associated with intensive care unit (ICU) admission in patients pulmonary infection and alter the respiratory microbiome

Liu,

Qi,

Shi

et al. 2024

Front. Microbiol.

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BackgroundThe Herpesviridae family contains several human-related viruses, which are able to establish colonizing and latency in the human body, posing a significant threat to the prognosis of patients. Pulmonary infections represent one of the predominant infectious diseases globally, characterized by diverse and multifaceted clinical manifestations that have consistently attracted clinician’s concern. However, the relationship of herpesviruses on the prognosis of pulmonary infections and the respiratory microbiota remains poorly understood.MethodsHere, we retrospectively analyzed respiratory samples from 100 patients with pulmonary infection detected by metagenomic next-generation sequencing (mNGS).ResultsEmploying mNGS, five herpesvirus species were detected: Human alphaherpesvirus 1 (HSV-1), Human gammaherpesvirus 4 (EBV), Human betaherpesvirus 5 (CMV), Human betaherpesvirus 7 (HHV-7), and Human betaherpesvirus 6B (HHV-6B). Regression analysis showed that the age and positivity of herpesviruses in patients were independently correlated with ICU admission rates. In addition, positivity of herpesvirus was related with increased ICU days and total hospital stay. The herpesvirus-positive group demonstrated markedly higher incidences of co-infections and fungi-positive, predominantly involving Pneumocystis jirovecii and Aspergillus fumigatus. Analysis of respiratory microbiota revealed a substantially altered community composition within the herpesvirus-positive group, and herpesviruses were significantly positively correlated with the diverse respiratory opportunistic pathogens.ConclusionOverall results substantiate that the active herpesviruses in patients with pulmonary infections were significantly associated with high ICU admission rate. Moreover, the herpesviruses promotes the dysbiosis of the respiratory microbiota and an increased proportion of co-infections. These insights could contribute to unraveling the underlying mechanisms connecting active herpesviruses to the progression of severe illnesses.

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Utility of clinical metagenomics in diagnosing malignancies in a cohort of patients with Epstein-Barr virus positivity

Cited by 3 publications

References 48 publications

Update on Fever of Unknown Origin in Children: Focus on Etiologies and Clinical Approach

Update on Fever of Unknown Origin in Children: Focus on Etiologies and Clinical Approach

Diagnostic performance of metagenomic next-generation sequencing among hematological malignancy patients with bloodstream infections after antimicrobial therapy

Active herpesviruses are associated with intensive care unit (ICU) admission in patients pulmonary infection and alter the respiratory microbiome

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