Utility of comet assay of DNA damage in the detection of malignant transformation of chronic liver cirrhosis

Saied, Narmin; Elmazny, Gehad Mohsen; Elhelaly, Rania; Farag, Raghda E.; Elwahab, Khaled Abd; Hashim, Ekbal Abo; Elzehery, Rasha

doi:10.1080/00365513.2023.2175327

Scandinavian Journal of Clinical and Laboratory Investigation

2023

DOI: 10.1080/00365513.2023.2175327

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Utility of comet assay of DNA damage in the detection of malignant transformation of chronic liver cirrhosis

Narmin Saied

Gehad Mohsen Elmazny

Rania Elhelaly

et al.

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Cited by 2 publications

References 27 publications

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Identification and in vitro genotoxicity assessment of forced degradation products of glimepiride and glyburide using HEK cell‐based COMET assay

Jain,

Aishwarya,

Wankhade

et al. 2024

Biomedical Chromatography

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This study focuses on characterizing the forced degradation products of antidiabetic drugs glimepiride (GMD) and glyburide (GBD), with previously unexplored genotoxicity. Drugs underwent stress induced by acid, base, and hydrogen peroxide. For GMD, impurities were profiled and isolated using Hypersil Gold C8 (250 × 10 mm, 5 μ) through semi‐preparative HPLC with a fraction collector. For GBD, impurity profiling was performed using semi‐preparative HPLC (Hypersil GOLD C18, 250 × 10 mm, 5 μ), and reverse‐phase flash chromatography (FP ECOFLEX C18 4 g column) for isolation. Although five GMD and three GBD impurities were detected, only three GMD and two GBD impurities were separated and assessed for purity using analytical RP‐HPLC with the purity percentages ranging from 96.6% to 99.9%. LC‐Orbitrap MS was used to identify these three GMD impurities (m/z: 408.122, 338.340, 381.160) and two GBD impurities (m/z: 369.065, 325.283). ProTox‐II in silico predictions classified all impurities as class 4 and 5, with no positive genotoxicity indications. In vitro comet assays, using HEK cells, indicated that for GMD, impurity 2 and impurity 5 were less genotoxic, whereas impurity 4 exhibited genotoxicity. For GBD, both impurities 1 and 3 were found to be genotoxic, with impurity 3 showing a higher level of genotoxicity than impurity 1.

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Identification and in vitro genotoxicity assessment of forced degradation products of glimepiride and glyburide using HEK cell‐based COMET assay

Jain,

Aishwarya,

Wankhade

et al. 2024

Biomedical Chromatography

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The Comet Assay Did Not Reveal a Decrease in DNA Damage to Lymphocytes when Exposed to X-Ray Radiation under the Action of Na-Cu-chlorophyllin

Romodin,

Ignatov

2023

Radiation biology. Radioecology

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The search for effective but non-toxic radioprotective agents remains the main task of radiobiology. Accor-ding to a number of reports, these may include preparations based on chlorophyll, in particular, chlorophyllin – a water-soluble product of its saponification. Since many researchers assign DNA damage a key role in the development of negative consequences of ionizing radiation, we conducted an experiment on X-ray irradiation of a suspension of lymphocytes in solutions of sodium-copper chlorophyllin in the concentration range of 5–100 micromoles. During it, using an alkaline modification of the gel electrophoresis method of individual cells, we found no significant differences in the DNA content in the tail and the tail moment of the DNA comets of irradiated lymphocytes incubated in chlorophyllin, compared with only irradiated cells. We explain this result by the fact that, most likely, chlorophyllin does not pass into the cell nuclei. And therefore it cannot show its antioxidant role in them.

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Utility of comet assay of DNA damage in the detection of malignant transformation of chronic liver cirrhosis

Cited by 2 publications

References 27 publications

Identification and in vitro genotoxicity assessment of forced degradation products of glimepiride and glyburide using HEK cell‐based COMET assay

Identification and in vitro genotoxicity assessment of forced degradation products of glimepiride and glyburide using HEK cell‐based COMET assay

The Comet Assay Did Not Reveal a Decrease in DNA Damage to Lymphocytes when Exposed to X-Ray Radiation under the Action of Na-Cu-chlorophyllin

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