Utility of Complete Mitochondrial Genomes in Phylogenetic Classification of the Species of Anopheles (Culicidae: Anophelinae)

Ghassemi-Khademi, Taghi; Oshaghi, Mohammad Ali; Vatandoost, Hassan; Madjdzadeh, Seyed Massoud; Gorouhi, Mohammad Amin

doi:10.18502/jad.v15i1.6483

Cited by 5 publications

(10 citation statements)

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“…All morphologically identified individuals were molecularly confirmed by barcoding. These results confirm the usefulness of the mitochondrial genome as a genetic marker [ 4 , 21 ]. In this study, the molecular identification of specimens collected in Roatán (Bay Islands), located more than 68 km from the mainland, was performed for the first time.…”

Section: Discussionsupporting

confidence: 82%

“…Anopheles albimanus is the most abundant and widespread species in the country [ 3 ]. The genus Anopheles (Culicidae: Anophelinae) includes almost 500 species grouped into at least 7 subgenera [ 4 , 5 ]. In Honduras, 13 species of the genus Anopheles belonging to 3 subgenera have been identified: Anopheles Anopheles ( Anopheles crucians , Anopheles pseudopunctipennis , Anopheles vestitipennis , Anopheles punctimacula , Anopheles neomaculipalpus , Anopheles apicimacula , Anopheles gabaldoni , and Anopheles grabhamii ), An .…”

Section: Introductionmentioning

confidence: 99%

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A Follow-Up to the Geographical Distribution of Anopheles Species in Malaria-Endemic and Non-Endemic Areas of Honduras

Escobar

Archaga

Reyes

et al. 2022

Insects

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Anopheles species are the vectors of malaria, one of the diseases with the greatest impact on the health of the inhabitants of the tropics. Due to their epidemiological relevance and biological complexity, monitoring of anopheline populations in current and former malaria-endemic areas is critical for malaria risk assessment. Recent efforts have described the anopheline species present in the main malaria foci in Honduras. This study updates and expands knowledge about Anopheles species composition, geographical distribution, and genetic diversity in the continental territory of Honduras as in the Bay Islands. Outdoor insect collections were carried out at 25 sites in eight municipalities in five departments of Honduras between 2018 and 2021. Specimens were identified using taxonomic keys. Partial COI gene sequences were used for molecular species identification and phylogenetic analyses. In addition, detection of Plasmodium DNA was carried out in 255 female mosquitoes. Overall, 288 Anopheles mosquitoes were collected from 8 municipalities. Eight species were morphologically identified. Anopheles albimanus was the most abundant and widely distributed species (79.5%). A subset of 175 partial COI gene sequences from 8 species was obtained. Taxonomic identifications were confirmed via sequence analysis. Anopheles albimanus and An. apicimacula showed the highest haplotype diversity and nucleotide variation, respectively. Phylogenetic clustering was found for An. argyritarsis and An. neomaculipalpus when compared with mosquitoes from other Neotropical countries. Plasmodium DNA was not detected in any of the mosquitoes tested. This report builds upon recent records of the distribution and diversity of Anopheles species in malaria-endemic and non-endemic areas of Honduras. New COI sequences are reported for three anopheline species. This is also the first report of COI sequences of An. albimanus collected on the island of Roatán with apparent gene flow relative to mainland populations.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

A Follow-Up to the Geographical Distribution of Anopheles Species in Malaria-Endemic and Non-Endemic Areas of Honduras

Escobar

Archaga

Reyes

et al. 2022

Insects

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“…coluzzii, and An. arabiensis); this grouping also occurred in the topologies of the phylogenetic trees 4,5,8 .…”

Section: Discussionmentioning

confidence: 57%

“…From the supercontig sequences of the subunit I of the sodium channel gene, the generated phylogenetic and evolutionary trees of the NaV gene showed a topology with some coincidences in relation to the phylogenetic trees for some species of anophelines 4,5,8 . In the topologies presented by these authors, as well as in the evolutionary trees of the NaV gene, An.…”

Section: Discussionmentioning

confidence: 97%

“…This disease is transmitted by mosquitoes of the genus Anopheles, which belongs to the Culicidae family. The subfamilies Anophelinae and Culicinae present evolutionary divergence over 100 million years, which occurred simultaneously, and are thus sister groups phylogenetically speaking 4,5 . Mosquitoes of the genera Aedes and Culex are grouped in the subfamily Culicinae, while those of the genus Anopheles belong to the subfamily Anophelinae.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic relationships of the supercontig of sodium channel subunit I (NaV) in 17 species of Anopheles (Diptera: Culicidae)

Santos

Bridi

Rafael

2022

Rev. Soc. Bras. Med. Trop.

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Background: Malaria is a global health problem and is transmitted by the Anopheles species. Due to the epidemiological importance of the genus, studies on biological, phylogenetic, and evolutionary aspects have contributed to the understanding of adaptation, vector capacity, and resistance to insecticides. The latter may result from different causes such as mutations in the gene that encodes the sodium channel (NaV).Methods: In this study, the NaV subunit I scaffold of 17 anopheline species was used to infer phylogenetic relationships of the genus Anopheles using Bayesian inference. The evolutionary phylogenetic tree of the NaV gene was aligned in the AliView program and analyzed utilizing Bayesian inference, using the software MrBayes. Results:The anophelines were grouped into five well-supported clusters: 1 -Anopheles darlingi and Anopheles albimanus; 2 -Anopheles sinensis and Anopheles atroparvus; 3 -Anopheles dirus; 4 -Anopheles minimus, Anopheles culicifacies, Anopheles funestus, Anopheles maculatus, and Anopheles stephensi; and 5 -Anopheles christyi, Anopheles epiroticus, Anopheles merus, Anopheles melas, Anopheles gambiae, Anopheles coluzzii, and Anopheles arabiensis. Conclusions:The topology confirms the phylogenetic relationships proposed in studies based on the genome of some anophelines and reflects the current taxonomy of the genus, which suggests that NaV undergoes selection pressure during the evolution of the species. These data are useful tools for inferring their ability to resist insecticides and also help in better understanding the evolutionary processes of the genus Anopheles.

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Wolbachia infection in native populations of Blattella germanica and Periplaneta americana

et al. 2023

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Cockroaches are significant pests worldwide, being important in medical, veterinary, and public health fields. Control of cockroaches is difficult because they have robust reproductive ability and high adaptability and are resistant to many insecticides. Wolbachia is an endosymbiont bacterium that infects the reproductive organs of approximately 70% of insect species and has become a promising biological agent for controlling insect pests. However, limited data on the presence or strain typing of Wolbachia in cockroaches are available. PCR amplification and sequencing of the wsp and gltA genes were used to study the presence, prevalence and molecular typing of Wolbachia in two main cockroach species, Blattella germanica (German cockroach) and Periplaneta americana (American cockroach), from different geographical locations of Iran. The Wolbachia endosymbiont was found only in 20.6% of German cockroaches while it was absent in American cockroach samples. Blast search and phylogenetic analysis revealed that the Wolbachia strain found in the German cockroach belongs to Wolbachia supergroup F. Further studies should investigate the symbiotic role of Wolbachia in cockroaches and determine whether lack of Wolbachia infection may increase this insect’s ability to tolerate or acquire various pathogens. Results of our study provide a foundation for continued work on interactions between cockroaches, bacterial endosymbionts, and pathogens.

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Utility of Complete Mitochondrial Genomes in Phylogenetic Classification of the Species of Anopheles (Culicidae: Anophelinae)

Cited by 5 publications

References 46 publications

A Follow-Up to the Geographical Distribution of Anopheles Species in Malaria-Endemic and Non-Endemic Areas of Honduras

A Follow-Up to the Geographical Distribution of Anopheles Species in Malaria-Endemic and Non-Endemic Areas of Honduras

Phylogenetic relationships of the supercontig of sodium channel subunit I (NaV) in 17 species of Anopheles (Diptera: Culicidae)

Wolbachia infection in native populations of Blattella germanica and Periplaneta americana

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