Utility of Hantzsch reaction for development of highly sensitive spectrofluorimetric method for determination of alfuzosin and terazosin in bulk, dosage forms and human plasma

Hammad, Mohamed A.; Omar, Mahmoud A.; Salman, Baher I.

doi:10.1002/bio.3292

Cited by 26 publications

(7 citation statements)

References 15 publications

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“…Into a centrifuge tube, 1.0 mL of human plasma was spiked with adequate amount of MOD solution. then furtherly 1 mL of methanol were added as protein precipitating agent [ 22 , 23 ]. The mixture was vortexed for 30 s and then completed to 10 mL.…”

Section: Methodsmentioning

confidence: 99%

Rapid One-Pot Microwave Assisted Green Synthesis Nitrogen Doped Carbon Quantum Dots as Fluorescent Precursor for Estimation of Modafinil as Post-Covid Neurological Drug in Human Plasma with Greenness Assessments

Salman

Hassan

et al. 2022

J Fluoresc

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The neuro-stimulant anti-narcoleptic drug as modafinil (MOD) is used to treatment neurological conditions caused by COVID-19. MOD was used to treatment narcolepsy, shift-work sleep disorder, and obstructive sleep apnea-related sleepiness. So, an innovative, quick, economical, selective, and ecologically friendly procedure was carried out. A highly sensitive N@CQDs technique was created from green Eruca sativa leaves in about 4 min using microwave synthesis at 700 w. The quantum yield of the synthesized N@CQDs was found to be 41.39%. By increasing the concentration of MOD, the quantum dots' fluorescence intensity was gradually quenched. After being excited at 445 nm, the fluorescence reading was recorded at 515 nm. The linear range was found to be in the range 50 – 700 ng mL−1 with lower limit of quantitation (LOQ) equal to 45.00 ng mL−1. The current method was fully validated and bio analytically according to (US-FDA and ICH) guidelines. Full characterization of the N@CQDs has been conducted by high resolution transmission electron microscope (HRTEM), Zeta potential measurement, fluorescence, UV–VIS, and FTIR spectroscopy. Various experimental variables including pH, QDs concentration and the reaction time were optimized. The proposed study is simply implemented for the therapeutic drug monitoring system (TDMS) and various clinical laboratories for further pharmacokinetic research.

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Section: Methodsmentioning

confidence: 99%

Rapid One-Pot Microwave Assisted Green Synthesis Nitrogen Doped Carbon Quantum Dots as Fluorescent Precursor for Estimation of Modafinil as Post-Covid Neurological Drug in Human Plasma with Greenness Assessments

Salman

Hassan

et al. 2022

J Fluoresc

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“…Acetonitrile was added (2 mL) as a protein precipitating agent. [10][11][12] The resultant supernatant solution was completed to 10 mL with distilled water and then centrifugation at 4000 rpm for 20 min. Furthermore, 2 mL of the supernatant was used to provide the final concentration range (40-500 ng mL −1 ) in the general procedure.…”

Section: Figurementioning

confidence: 99%

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

et al. 2019

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Tigecycline (TIGE) is the newest tetracycline derivative antibiotic with low toxicity, it is used for management of infectious diseases caused by Gram-positive and Gramnegative bacteria. Hence, an efficient, selective and sensitive method was developed for analysis of TIGE in commercial formulations, human plasma and urine. The spectrofluorimetric technique based on the reaction of secondary amine moiety in TIGE with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in slightly alkaline medium producing a highly fluorescent product measured at 540 nm (λ ex at 470 nm) after heating for 15 min at 75°C. The proposed strategy was upgraded and approved by ICH rules and bio-analytical validated using US-FDA recommendations. A linear relationship between fluorescence intensity and TIGE concentration was observed over the concentration range 40-500 ng mL −1 with limit of quantification (LOQ) 21.09 ng mL −1 and limit of detection (LOD) 6.96 ng mL −1 .The ultra-affectability and high selectivity of the proposed strategy permits analysis of TIGE in dosage form, human plasma and urine samples with good recovery ranged from 97.23% to 98.72% and from 99.36% to 99.80% respectively, without any interfering from matrix components. Also, the developed strategy was used to examine the stability of TIGE in human plasma and applied for pharmacokinetic investigation of TIGE.

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“…The Hantzsch reaction is widely used for both spectrophotometric and spectrofluorimetric analysis of drugs. [25][26][27][28] The developed method was dependent on the interaction between aliphatic primary amino groups found in heptaminol and ethyl acetoacetate and formaldehyde, giving a yellow coloured and fluorescent dihydropyridine derivative that showed a maximum fluorescence at 416 nm (excitation at 350 nm) (Figures 2 and 3).…”

Section: Resultsmentioning

confidence: 99%

Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma

2020

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A sensitive, simple, accurate and less expensive fluorimetric method was designed and validated for analysis of heptaminol HCl in both its pure and dosage forms, as well as in human plasma. The main principle used in the proposed approach was the condensation reaction between heptaminol's primary amino moiety and ethyl acetoacetate/formaldehyde reagents, giving a derivative that was highly fluorescent at 416 nm after excitation at 350 nm. Various experimental parameters that affected either the product's development or its stability were evaluated and optimized. The constructed calibration curve was linear over the range 0.2–2 μg/ml, with a good correlation coefficient (0.9996). Both the calculated limit of detection and limit of quantitation were 0.06 and 0.18 μg/ml, respectively. The presented approach was a success when used to determine Corasore® tablets and was validated according to International Council for Harmonisation guidelines.

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Utility of Hantzsch reaction for development of highly sensitive spectrofluorimetric method for determination of alfuzosin and terazosin in bulk, dosage forms and human plasma

Cited by 26 publications

References 15 publications

Rapid One-Pot Microwave Assisted Green Synthesis Nitrogen Doped Carbon Quantum Dots as Fluorescent Precursor for Estimation of Modafinil as Post-Covid Neurological Drug in Human Plasma with Greenness Assessments

Rapid One-Pot Microwave Assisted Green Synthesis Nitrogen Doped Carbon Quantum Dots as Fluorescent Precursor for Estimation of Modafinil as Post-Covid Neurological Drug in Human Plasma with Greenness Assessments

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma

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