Utility of isotachophoresis–capillary zone electrophoresis, mass spectrometry and high-performance size-exclusion chromatography for monitoring of interleukin-6 dimer formation

Gysler, Jens; Mazereeuw, M.; Helk, Bernhard; Heitzmann, Michael; Jaehde, Ulrich; Schunack, Walter; Tjaden, U.R.; Greef, J. van der

doi:10.1016/s0021-9673(99)00257-5

Cited by 15 publications

(13 citation statements)

References 18 publications

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“…Application of hydrodynamic counterflow together with ITP technique made the complete length of capillary available for the separation step and resulted in the reduction of the LOQ of the method to the concentration range of 10 −8 M . One year later, Gysler and colleagues applied an ITP‐CZE method to monitor dimmer formation as a physical instability of rhIL‐6. The high sensitivity of the developed method owing to the application of ITP technique made it useful to separate monomer and dimmer forms of rhIL‐6.…”

Section: Ce‐based Methods Applied In the Quality Control Of Biopharmamentioning

confidence: 99%

“…Gysler and co‐workers used a CZE‐ESI‐MS method to gain detailed information about the formation of dimmer complexes of rhIL‐6 under elevated temperature. Xin et al described the usefulness of a home‐built CE‐ESI‐ion cyclotron resonance‐MS instrument for the separation and structural identification of three peptides, including angiotensin III, octreotide, and elastin chemotactic, in a mixture.…”

Section: Ce‐based Methods Applied In the Quality Control Of Biopharmamentioning

confidence: 99%

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The potential of the capillary electrophoresis techniques for quality control of biopharmaceuticals—A review

Tamizi

Jouyban

2015

Electrophoresis

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CE is considered as a powerful technique in biopharmaceutical industry, owing to its inherent advantages such as high resolution, efficient separation, and its flexibility to couple with high-sensitive detecting methods. Present review provides a summary of the applications of CE-based methods in the quality control of biopharmaceuticals according to the papers published between 1994 and July 2014. This article is divided into the sections based on different CE modes applied in the analysis of biopharmaceuticals and gives detailed information about the employed experimental conditions. At the end some overall conclusions and perspectives are given.

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Section: Ce‐based Methods Applied In the Quality Control Of Biopharmamentioning

confidence: 99%

Section: Ce‐based Methods Applied In the Quality Control Of Biopharmamentioning

confidence: 99%

The potential of the capillary electrophoresis techniques for quality control of biopharmaceuticals—A review

Tamizi

Jouyban

2015

Electrophoresis

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“…Gysler et al [88] investigated the utility of ITP-CZE and high performance size-exclusion chromatography (HPSEC) to analyse dimeric and monomeric rhIL-6. Dimer formation was evaluated over a wide range of pH and temperature.…”

Section: Formulation Studiesmentioning

confidence: 99%

Capillary Electrophoresis in the Development of Recombinant Protein Biopharmaceuticals

Chen¹,

Canova-Davis²

2001

CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

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The understanding of the physicochemical properties of proteins in solution is required to understand the separations taking place in the capillary. For example, during isoelectric focusing, it is important to ensure the lack of protein precipitation during fOCUSing and mobilization otherwise artifacts occur and the true resolution of charge variants is not achieved. Similarly, buffer conditions, including additives, composition of buffer, and pH can influence the resolution of product variants. With diligent efforts one can achieve desired separations. As knowledge of buffer conditions and use of these techniques increase, the methods will be improved.MECC can be useful for the characterization of recombinant proteins and monoclonal antibodies. However, the relatively long analyte migration times may be a deterrent for routine use in quality control situations. Pharmaceutical companies will employ new techniques which provide advantages over current technology. CE has advantages in certain applications. Applications which use slab gel techniques, IEF and SDS-PAGE can be replaced by the CE methods for formulation development, characterization of charge isoforms, and for purity determinations. CZE and clEF can be used for rapid identity tests which do not require peptide mapping. CZE has a tremendous advantage in analyzing basic proteins, and CZE and CE-SDS can be used to quantify product in the presence of interfering excipients. It is the responsibility of the analytical chemist to determine applications which are appropriate for CEo It is apparent that these are currently being defined for the biotechnology industry, and the usage of CE should continue to increase.

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“…ITP preconcentration with hydrodynamic counterflow was used to enhance the detectability of amitriptyline and metoprolol in CZE (LE = BGE: 10 mM NaOH; TE: 6.1 mM tetrapentylammonium hydroxide, both adjusted to pH 2.75 with phosphoric acid) [95], to determine trace concentrations of recombinant human interleukin-3, recombinant human IL-6 and various basic model proteins (LE = BGE: 20 mM triethylamine (TEA) adjusted to pH 4.2 with acetic acid; TE: 20 mM b-alanine adjusted to pH 4.5 with acetic acid) [96], to monitor the formation of the interleukin-6 dimer (LE = BGE: 20 mM ammonium acetate adjusted to pH 4.2 with acetic acid; TE: 10 mM acetic acid) [97], to characterize recombinant cytokine fragments [98] and to preconcentrate a new cholinesterase inhibitor (NXX-066) prior to CZE analysis with LIF detection (LE = BGE: 10 mM NaOH adjusted to pH 2.72 with phosphoric acid; TE: 6.13 mM tetrahexylammonium chloride adjusted to pH 2.72 with phosphoric acid, 37,5% v/v methanol) [99]. The column coupling technique is being used for a long time to enhance the selectivity and sensitivity of ITP by combining two different ITP electrolyte systems.…”

Section: Transient Itp and Combined Techniquesmentioning

confidence: 99%

Recent progress in capillary isotachophoresis

Gebauer

Boček

2000

Electrophoresis

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Utility of isotachophoresis–capillary zone electrophoresis, mass spectrometry and high-performance size-exclusion chromatography for monitoring of interleukin-6 dimer formation

Cited by 15 publications

References 18 publications

The potential of the capillary electrophoresis techniques for quality control of biopharmaceuticals—A review

The potential of the capillary electrophoresis techniques for quality control of biopharmaceuticals—A review

Capillary Electrophoresis in the Development of Recombinant Protein Biopharmaceuticals

Recent progress in capillary isotachophoresis

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