Utility of multiplex PCR syndromic panel for respiratory infections in the diagnosis of acute bacterial meningitis

Egea, María Ángeles Asencio; Pitera, Jorge Gaitán; González, Jean Carlos Méndez; Vaquero, María Huertas

doi:10.1016/j.eimce.2021.08.009

Enfermedades infecciosas y microbiologia clinica (English ed.)

2021

DOI: 10.1016/j.eimce.2021.08.009

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Utility of multiplex PCR syndromic panel for respiratory infections in the diagnosis of acute bacterial meningitis

María Ángeles Asencio Egea¹,

Jorge Gaitán Pitera²,

Jean Carlos Méndez González³

et al.

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2024

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An affordable and simple method for DNA extraction from agarose suitable for downstream applications

Sánchez-Flores,

Sandoval-Cabrera,

Alarcón-Valdés

et al. 2024

Preprint

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DNA serves as the foundation for molecular biology, leading to the development of numerous molecular techniques. Often, these techniques necessitate the separation and visualization of specific DNA regions. Electrophoresis provides a solution for this requirement. However, the purification of DNA from agarose gels presents a significant challenge, both in terms of complexity and cost. Therefore, here we propose a protocol that is both cost-effective and efficient. GFP bands in the PJet vector were visualized and excised for DNA extraction using five treatments. Only the treatment involving TAE buffer failed, while others partially or fully dissolved the gel, aiding DNA recovery. Treatments 2, 4, and 5 showed defined bands without DNA degradation. A nested PCR confirmed the suitability of the recovered DNA for further applications, with only treatments 4 and 5 being amplified. Two additional DNA samples were successfully extracted from a 1% agarose gel using methods 4 and 5, demonstrating their effectiveness.

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An affordable and simple method for DNA extraction from agarose suitable for downstream applications

Sánchez-Flores,

Sandoval-Cabrera,

Alarcón-Valdés

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Utility of multiplex PCR syndromic panel for respiratory infections in the diagnosis of acute bacterial meningitis

Cited by 1 publication

References 6 publications

An affordable and simple method for DNA extraction from agarose suitable for downstream applications

An affordable and simple method for DNA extraction from agarose suitable for downstream applications

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