Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria

Pandey, Gaurav; Singh, Harshinder; Chaturvedi, Saurabh; Hatti, Manjunath; Kumar, Alok; Mishra, Ravi; Mishra, Prabhakar; Krishna, Vibhor; Bhadauria, Arun Singh; Mohindra, Samir; Misra, Durga Prasanna; Saraswat, Vivek A.; Agarwal, Vikas

doi:10.1038/s41598-021-99276-y

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Normally, CD64 expression is very low in neutrophils, but the expression is significantly induced in peripheral blood neutrophils during bacterial infection and helps in neutrophil phagocytosis and sterilization [ 19 , 20 ]. Many studies show that CD64 has high specificity in bacterial infection, which is helpful in early diagnosis of bacterial infection and the degree of infection [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Clinical Value of Neutrophil CD64 Index, PCT, and CRP in Acute Pancreatitis Complicated with Abdominal Infection

Wang

Tang

et al. 2022

Diagnostics

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Objective: To study the clinical diagnostic value of neutrophil CD64 index, PCT, and CRP in patients with acute pancreatitis with abdominal infection. Methods: A number of patients with acute pancreatitis (n = 234) participated in the study. According to the infection and health conditions, they were further divided into the non-infection group (n = 122), infection group (n = 78), and sepsis group (n = 34), and 40 healthy subjects were selected in the control group (n = 40). Expression levels of infection indexes, such as CD64 index, PCT, and CRP, were detected and compared. ROC curves were drawn to compare the efficacy of each index in the diagnosis of acute pancreatitis with abdominal infection and sepsis. The study was retrospectively registered under the China Clinical Trial Registry as a trial number ChiCTR2100054308. Results: All indexes were significantly higher in three clinical groups than the healthy control group (p < 0.05). The CD64 index, CD64 positive rate, and PCT in the infected group were significantly higher than those in the uninfected group (ALL p < 0.05). The PCT of patients infected with Gram-negative bacteria was significantly higher than that of Gram-positive bacteria-infected patients (p < 0.05). CD64 index had the best diagnostic efficiency for acute pancreatitis infection, with 82.14% sensitivity, 88.51% specificity, and 0.707 Youden indexes. The CD64 Youden index (0.780) for sepsis diagnosis was the highest, while the AUC of PCT was the highest (0.897). Conclusion: CD64 index combined with PCT has good sensitivity and specificity in diagnosing acute pancreatitis infection and sepsis.

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Section: Introductionmentioning

confidence: 99%

Clinical Value of Neutrophil CD64 Index, PCT, and CRP in Acute Pancreatitis Complicated with Abdominal Infection

Wang

Tang

et al. 2022

Diagnostics

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“…Pander G et al. indicated that quantitative detection of nCD64 in patients with severe alcoholic hepatitis could more accurately identify systemic bacterial infections and inflammation compared to other inflammatory markers ( 22 ). Consistently, our study also showed that KTRs with bacterial infections had significantly higher nCD64 and CD64 index than those with viral infections.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, some researches showed that nCD64 expression performed good diagnostic efficacy in bacterial infections and sepsis ( 22 ). In a prospective observational study, it concluded that serial measurement of nCD64 expression could facilitate sepsis diagnosis and monitor the clinical course in critically ill patients ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

Standardization of neutrophil CD64 and monocyte HLA-DR measurement and its application in immune monitoring in kidney transplantation

et al. 2022

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BackgroundInfections cause high mortality in kidney transplant recipients (KTRs). The expressions of neutrophil CD64 (nCD64) and monocyte HLA-DR (mHLA-DR) provide direct evidence of immune status and can be used to evaluate the severity of infection. However, the intensities of nCD64 and mHLA-DR detected by flow cytometry (FCM) are commonly measured by mean fluorescence intensities (MFIs), which are relative values, thus limiting their application. We aimed to standardize nCD64 and mHLA-DR expression using molecules of equivalent soluble fluorochrome (MESF) and to explore their role in immune monitoring for KTRs with infection.MethodsThe study included 50 KTRs diagnosed with infection, 65 immunologically stable KTRs and 26 healthy controls. The blood samples were collected and measured simultaneously by four FCM protocols at different flow cytometers. The MFIs of nCD64 and mHLA-DR were converted into MESF by Phycoerythrin (PE) Fluorescence Quantitation Kit. The intraclass correlation coefficients (ICCs) and the Bland-Altman plots were used to evaluate the reliability between the four FCM protocols. MESFs of nCD64 and mHLA-DR, nCD64 index and sepsis index (SI) with the TBNK panel were used to evaluate the immune status. Comparisons among multiple groups were performed with ANOVA one-way analysis. Receiver operating characteristics (ROC) curve analysis was performed to diagnose infection or sepsis. Univariate and multivariate logistic analysis examined associations of the immune status with infection.ResultsMESFs of nCD64 and mHLA-DR measured by four protocols had excellent reliability (ICCs 0.993 and 0.957, respectively). The nCD64, CD64 index and SI in infection group were significantly higher than those of stable KTRs group. Patients with sepsis had lower mHLA-DR but higher SI than non-sepsis patients. ROC analysis indicated that nCD64 had the highest area under the curve (AUC) for infection, and that mHLA-DR had the highest AUC for sepsis. Logistic analysis indicated that nCD64 > 3089 and B cells counts were independent risk factors for infection.ConclusionThe standardization of nCD64 and mHLA-DR made it available for widespread application. MESFs of nCD64 and mHLA-DR had good diagnostic performance on infection and sepsis, respectively, which could be promising indicators for immune status of KTRs and contributed to individualized treatment.

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“… 65 Numerous studies have shown an increase in neutrophils in the circulation and in the liver of ALD patients, and neutrophil levels correlate with disease severity and poor clinical outcomes in ALD patients. 66 , 67 Chronic ethanol feeding in mice induces peripheral blood and liver neutrophil infiltration, which is associated with liver injury. 68 Neutrophil aggregation is thought to cause severe hepatocyte damage by promoting macrophage recruitment and interaction with antigen-presenting cells.…”

Section: Neutrophil Recruitment In Aldmentioning

confidence: 99%

Platelets in Alcohol-Associated Liver Disease: Interaction With Neutrophils

Wang,

Peng

et al. 2024

Cellular and Molecular Gastroenterology and Hepatology

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Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria

Cited by 9 publications

References 33 publications

Clinical Value of Neutrophil CD64 Index, PCT, and CRP in Acute Pancreatitis Complicated with Abdominal Infection

Clinical Value of Neutrophil CD64 Index, PCT, and CRP in Acute Pancreatitis Complicated with Abdominal Infection

Standardization of neutrophil CD64 and monocyte HLA-DR measurement and its application in immune monitoring in kidney transplantation

Platelets in Alcohol-Associated Liver Disease: Interaction With Neutrophils

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