Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination

Abstract

Fast hexavalent chromium (Cr(VI)) determination is important for environmental risk and health-related considerations. We used a microbial fuel cell-based biosensor inoculated with a facultatively anaerobic, Cr(VI)-reducing, and exoelectrogenic Ochrobactrum anthropi YC152 to determine the Cr(VI) concentration in water. The results indicated that O. anthropi YC152 exhibited high adaptability to pH, temperature, salinity, and water quality under anaerobic conditions. The stable performance of the microbial fuel cell (MFC)-based biosensor indicated its potential as a reliable biosensor system. The MFC voltage decreased as the Cr(VI) concentration in the MFC increased. Two satisfactory linear relationships were observed between the Cr(VI) concentration and voltage output for various Cr(VI) concentration ranges (0.0125–0.3 mg/L and 0.3–5 mg/L). The MFC biosensor is a simple device that can accurately measure Cr(VI) concentrations in drinking water, groundwater, and electroplating wastewater in 45 min with low deviations (<10%). The use of the biosensor can help in preventing the violation of effluent regulations and the maximum allowable concentration of Cr(VI) in water. Thus, the developed MFC biosensor has potential as an early warning detection device for Cr(VI) determination even if O. anthropi YC152 is a possible opportunistic pathogen.

Recent Advances in Electrochemical Monitoring of Chromium

“…Nevertheless, the proposed MFCs showed limitations in Cr(VI) detection at higher pH conditions. Which was overcame later [ 120 , 121 ]. Guey-Horng Wang et al [ 120 ] suggested the use of an MFC based biosensor inoculated with a facultatively anaerobic, a Cr(VI)-reducing Ochrobactrum anthropi YC152 for Cr(VI) measurements in water.…”

“…Which was overcame later [ 120 , 121 ]. Guey-Horng Wang et al [ 120 ] suggested the use of an MFC based biosensor inoculated with a facultatively anaerobic, a Cr(VI)-reducing Ochrobactrum anthropi YC152 for Cr(VI) measurements in water. The Ochrobactrum anthropi YC152 displayed high adaptability to pH (5–8), salinity, temperature and quality of water under anaerobic conditions.…”

Critical review on microbial fuel cells for concomitant reduction of hexavalent chromium and bioelectricity generation

“…An MFC‐based biosensor for the determination of Cr(VI) in water was designed by inoculating the facultatively anaerobic, Cr(VI)‐reducing exoelectrogenic bacterium Ochrobactrum anthropi YC152 into the anode compartment . This chromate‐reducing bacterium converted the organics to carbon dioxide and also Cr(VI) to Cr(III), with the generation of protons and electrons.…”

“…79 An MFC-based biosensor for the determination of Cr(VI) in water was designed by inoculating the facultatively anaerobic, Cr(VI)-reducing exoelectrogenic bacterium Ochrobactrum anthropi YC152 into the anode compartment. 80 This chromatereducing bacterium converted the organics to carbon dioxide and also Cr(VI) to Cr(III), with the generation of protons and electrons. The biosensor was based on the concept that the higher the concentration of Cr(VI) in the anode, the fewer electrons are transferred to the cathode at constant organic concentration.…”

Microbial Fuels Cell-Based Biosensor for Toxicity Detection: A Review

“…In one case, the inoculum source is a compound substance such as anaerobic sludge, soil, or domestic wastewater, which provides a bacterial consortium for the anode chamber [ 29 , 30 , 31 ]. Alternatively, pure cultures have been used as anode inoculum in recent studies [ 32 , 33 , 34 ].…”