Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

Diederen, Bram M. W.; Kluytmans, Jan; Vandenbroucke-Grauls, Christina M. J. E.; Peeters, Marcel

doi:10.1128/jcm.01196-07

Cited by 73 publications

(40 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Amplification of Legionella DNA by PCR can provide results within a short time. It also has the potential to detect infections caused by any Le-gionella species and serogroups, in addition to detect non-culturable legionella and allow the manipulation of a large number of samples [3,9,59,64]. The detection of other species besides L. pneumophila is relevant to the diagnosis of Legionnaires' disease, since this disease can be caused by other species, such as L. longbeachae and L. bozemanii.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, several authors [1,3,50,54] have described the isolation of Legionella spp. from showers, cooling towers and boilers.…”

Section: Discussionmentioning

confidence: 99%

“…The development of new and rapid assays that combine both specific detection and viability criteria is essential for monitoring water quality and legionellosis prevention [3,24]. A rapid, sensitive, and specific method for the detection of Legionella is clearly important to identify infected individuals and to expedite cleanup of contaminated water systems (reduction in disinfecting treatment cost) in order to prevent additional cases of infection [25,47].…”

Section: Discussionmentioning

confidence: 99%

“…The genus Legionella comprises 71 distinct serogroups from more than 50 known species [2][3][4] and new species are frequently described [5][6][7]. Legionella pneumophila, was first recognized in 1977 following an epidemic of acute pneumonia in Philadelphia [8], is the etiological agent of the majority of cases of legionellosis, and the best part of cases have been attributed to L. pneumophila serogroup 1 [3,9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Borges¹,

Simes²,

Martínez‐Murcia³

et al. 2012

MICROBIOLOGY

View full text Add to dashboard Cite

Infections caused by Legionella spp. are considered at the present time, an emerging public health problem and are linked to high rates of mortality and morbidity, if not properly treated. In this study were analyzed 54 samples of water from 8 counties at Northern Portugal, with the aim of obtaining a collection of strains of the genus Legionella and to characterize them genetically and phenotypically. Another objective of this study was to evaluate the effectiveness of the technique of cultivation, a standard method according to International Organization for Standardization ISO 11731:1998, for detection and enumeration of species of Legionella. For laboratory processing, after the filtration of samples (1 L), the filtrate was resuspended in sterile distilled water (5 ml). Heat treatment for selective inhibition of non-Legionella bacteria was performed. Subsequently, 100 μl of the suspension was spread in GVPC selective agar medium, and incubated (7 to 10 days) at 37 ℃. Colonies that were morphologically characteristic of the genus were sub-cultured onto BCYE agar and blood agar for verification. According to the procedure recommended by the standard method, only the colonies which grew in BCYE agar and not on blood agar were considered as suspected Legionella strains. The identification of these initially selected colonies was performed by sequencing the 16S rRNA gene, which revealed that none of the isolates were identified as belonging to the genus Legionella. However, through the ISO 11731:1998 they were interpreted as positive, corresponding therefore to "false-positive" results. The methods used in this study allowed the isolation of a number of isolates (40), which form an independent group of all genus of the family Chitinophagaceae outlined so far, and that by their phylogenetic distance might be a genus not yet described and therefore a new species. The results obtained, highlighted the importance of using culture and genetic methods in parallel for the proper identification of microorganisms.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Moreover, several authors [1,3,50,54] have described the isolation of Legionella spp. from showers, cooling towers and boilers.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Borges¹,

Simes²,

Martínez‐Murcia³

et al. 2012

MICROBIOLOGY

View full text Add to dashboard Cite

show abstract

“…Se ha probado la detección de antígeno urinario y las amplificaciones de ácido nucleico en muestras respiratorias (64). La PCRtr ofrece ventajas como alta sensibilidad, obtención rápida de resultados y el potencial de detectar infecciones causadas por varios serogrupos de Legionella pneumophila y no -pneumophila (65,66).…”

Section: Diagnostico Microbiológico Clínicounclassified

Bodas de plata de la reacción en cadena de la polimerasa (PCR)

Pinilla

Cubillos

Rodríguez³

2008

nova

View full text Add to dashboard Cite

ResumenInvenciones verdaderamente revolucionarias han promovido el cambio de pensamiento y la manera de trabajar en el ámbito del laboratorio. Una de estas invenciones es la reacción en cadena de la polimerasa, la cual ha aportado de manera significativa al conocimiento científico. Las diferentes metodologías que aplican la reacción en cadena de la polimerasa han permitido a los investigadores manipular la información genética de los organismos, facilitando procedimientos como la clonación y la secuenciación, entre otros, lo cual agilizó significativamente los resultados del Proyecto Genoma Humano. Existe diversidad de variantes de la reacción en cadena de la polimerasa convencional. Este escrito tiene por objetivo presentar una revisión sobre el tema, especialmente sobre la reacción en cadena de la polimerasa en tiempo real, debido a las ventajas que ofrece.Palabras clave: agentes intercalantes, cuantificación absoluta, cuantificación relativa, curvas de calibración, gen normalizador, sondas fluorogénicas. Abstract Silver anniversary of the Polymerase Chain Reaction (PCR)Truly revolutionary inventions have promoted the change of thought and the way to work in the laboratory. One of these inventions is the polymerase chain reaction (PCR), which has contributed significantly to the scientific knowledge. The different methodologies that use the polymerase chain reaction have allowed investigators to manipulate the genetic information of organisms, facilitating procedures like cloning and sequencing, among others, which sped the Human Genome Project results significantly. There are several variants of the conventional polymerase chain reaction. This work tries to present a revision on the subject, especially on the Real-time polymerase chain reaction, due to the advantages that it offers.

show abstract

Rapid PCR Screening Methods

Babady

Cockerill

Patel

2011

Molecular Techniques for the Study of Hospital‐Acquired Infection

View full text Add to dashboard Cite

Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

Abstract: The main aim of our study was to determine the added value of PCR for the diagnosis of Legionnaires' disease (

Cited by 73 publications

References 38 publications

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Bodas de plata de la reacción en cadena de la polimerasa (PCR)

Rapid PCR Screening Methods

Contact Info

Product

Resources

About