Utility of sheathless capillary electrophoresis-mass spectrometry for metabolic profiling of limited sample amounts

Zhang, Wei; Guled, Faisa; Hankemeier, Thomas; Ramautar, Rawi

doi:10.1016/j.jchromb.2018.12.004

Cited by 26 publications

(17 citation statements)

References 21 publications

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“…A major challenge in metabolomics is still the efficient analysis of polar and charged metabolites in biological samples, even more so when such samples are limited in volume or biomass. Over the past few years, CE‐MS emerged as a strong analytical technique for the profiling of metabolites in small‐volume biological samples, ranging from body fluids of small animal models to low numbers of mammalian cells [24–26]. However, the typical volume needed in sample vials of (commercial) CE instruments is in the order of 5 to 10 (or more) microliters, while only nanoliters are injected into the CE system.…”

Section: Technological Developmentsmentioning

confidence: 99%

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

Zhang

Ramautar

2020

Electrophoresis

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Capillary electrophoresis‐mass spectrometry (CE‐MS) is now a mature analytical technique in metabolomics, notably for the efficient profiling of polar and charged metabolites. Over the past few years, (further) progress has been made in the design of improved interfacing techniques for coupling CE to MS; also, in the development of CE‐MS approaches for profiling metabolites in volume‐restricted samples, and in strategies that further enhance the metabolic coverage. In this article, which is a follow‐up of a previous review article covering the years 2016–2018 (Electrophoresis 2019, 40, 165–179), the main (technological) developments in CE‐MS methods and strategies for metabolomics are discussed covering the literature from July 2018 to June 2020. Representative examples highlight the utility of CE‐MS in the fields of biomedical, clinical, microbial, plant and food metabolomics. A complete overview of recent CE‐MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, some general conclusions and perspectives are given.

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Section: Technological Developmentsmentioning

confidence: 99%

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

Zhang

Ramautar

2020

Electrophoresis

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“…The sample volume of plasma and cerebrospinal fluid, especially from animal models, can be rather low. As a result, this raised the interest in techniques that are able to analyze limited-volume samples[88,91]. The possibilities of CE-MS to analyze low sample volumes is demonstrated by Zhang et al[91], who detect acceptable metabolic read-outs based on only 5 HepG2 cells, shown in Figure4.…”

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confidence: 99%

Analytical Techniques for Metabolomic Studies: A Review

et al. 2019

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Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.

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“…The supernatant from each tube was then filtered with centrifugal filters with 3 kDa cutoffs and centrifuged using 10 000 × g at 4 ο C for 1.5 h. Filtered cell lysates were then transferred and stored at −80 ο C prior to further sample preparation. The cell lysate samples were processed in a similar manner as previously described . The cell lysate obtained corresponded to a cell density of 50 000 cells/50 µL and was further diluted to 10 000, 5000, 2500, 1000, and 500 cells/50 µL with ice‐cold methanol/water (80:20, v/v).…”

Section: Methodsmentioning

confidence: 99%

Profiling nucleotides in low numbers of mammalian cells by sheathless CE–MS in positive ion mode: Circumventing corona discharge

et al. 2020

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Negative ion mode nano‐ESI–MS is often considered for the analysis of acidic compounds, including nucleotides. However, under high aqueous separation conditions, corona discharge is frequently observed at emitter tips, which may result in low ion abundances and reduced nano‐ESI needle emitter lifetimes. In this work, we introduce a sheathless CE‐MS method for the highly efficient and sensitive analysis of nucleotides employing ESI in positive ion mode, thereby fully circumventing corona discharge. By using a background electrolyte of 16 mM ammonium acetate (pH 9.7) a mixture of 12 nucleotides, composed of mono‐, di‐, and tri‐phosphates, could be efficiently analyzed with plate numbers per meter above 220 000 and with LODs in the range from 0.06 to 1.3 nM, corresponding to 0.4 to 8.6 attomole, when using an injection volume of about 6.5 nL only. The utility of the method was demonstrated for the profiling of nucleotides in low numbers of mammalian cells using HepG2 cells as a model system. Endogenous nucleotides could be efficiently analyzed in extracts from 50 000 down to 500 HepG2 cells only. Moreover, apart from nucleotides, also some nicotinamide‐adenine dinucleotides and amino acids could be analyzed under these conditions, thereby clearly illustrating the utility of this approach for metabolic profiling of low amounts of biological material.

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Utility of sheathless capillary electrophoresis-mass spectrometry for metabolic profiling of limited sample amounts

Cited by 26 publications

References 21 publications

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

Analytical Techniques for Metabolomic Studies: A Review

Profiling nucleotides in low numbers of mammalian cells by sheathless CE–MS in positive ion mode: Circumventing corona discharge

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