Utility of Specimen Magnetic Resonance Imaging (Mri) in Clinical Neuropathology

Boyko, Orest B.; Alston, Sebastian R.; Fuller, Gregory N.; Hulette, Christine M.; Burger, Peter C.

doi:10.1097/00005072-198905000-00034

Cited by 29 publications

(38 citation statements)

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“…One study of over 200 cases emphasized its ability to view the brain in many planes, to detect small lesions, and to evaluate the extent of edema (11). Other authors have reported its role following infant death when child abuse is suspected (12) and in the detection of MIs in formalin-fixed dissected hearts (13).…”

Section: Discussionmentioning

confidence: 99%

Postmortem whole-body magnetic resonance imaging as an adjunct to autopsy: Preliminary clinical experience

Patriquin

Kassarjian

O’Brien

et al. 2001

J. Magn. Reson. Imaging

130

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Section: Discussionmentioning

confidence: 99%

Postmortem whole-body magnetic resonance imaging as an adjunct to autopsy: Preliminary clinical experience

Patriquin

Kassarjian

O’Brien

et al. 2001

J. Magn. Reson. Imaging

130

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“…The relationship between medical disorders, high intensity lesions and atrophy is complex and needs to be better elucidated. High intensity lesions occur predominantly in white matter regions of the brain and they represent a spectrum of pathological changes including ischemia, demyelination and edema (Boyko et al 1994;Drayer 1988). Results from the Cardiovascular Health Study indicate that the frequent correlates of these lesions include age, silent stroke, hypertension and FEV 1 (Longstreth et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Atrophy and High Intensity Lesions Complementary Neurobiological Mechanisms in Late-Life Major Depression

Kumar

Bilker

Jin

et al. 2000

Neuropsychopharmacology

166

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The primary objective of our study was to examine the role of atrophy, high intensity lesions and medical comorbidity in the pathophysiology of major depressive disorder in the elderly (late-life MDD). Our sample was comprised of 51 patients with late-life MDD and 30 non-depressed controls.All subjects were scanned on 1.5 tesla magnetic resonance imaging scanner (MRI) Depression is one of the most common mental disorders in the elderly (Alexopolous et al. 1988;Beck and Koenig 1996;Blazer et al. 1987;Blazer 1989Blazer , 1994Koenig et al. 1993;Oxman et al. 1987;Parmalee et al. 1989;Ruegg et al. 1988;Sherbourne et al. 1994). The prevalence of major depressive disorder (MDD) is estimated to be approximately 1-2 percent in community settings and strikingly higher in inpatient and long term care settings (Blazer et al. 1987;Koenig et al. 1993;Parmalee et al. 1989). The prevalence of other clinically significant forms of depression, that do not meet the severity criteria for MDD, lies between 5 and 15 percent in all major clinical settings (Beck and Koenig 1996;Blazer et al. 1987, Blazer 1994Parmalee et al. 1989). Anatomical and physiological neuroimaging approaches have been utilized to study the neurobiological correlates of MDD and minor depression in the elderly (Coffey et al. 1990;Coffey et al. 1993;Greenwald et al. 1996;Krishnan et al. 1988;Krishnan 1993;Kumar et al. 1997a;Lesser et al. 1994;Sackheim et al. 1993;Sheline et al. 1996). Glucose hypometabolism and Lesser et al. 1994;Sackheim et al. 1993). Using magnetic resonance imaging (MRI), investigators have demonstrated neuroanatomical abnormalities including smaller focal brain volumes and larger high intensity lesion volumes in patients with both late-life major and minor depression when compared with non-depressed controls (Coffey et al. 1990;Coffey et al. 1993;Greenwald et al. 1996;Krishnan et al. 1988;Krishnan 1993;Kumar et al. 1997aKumar et al. , 1997bKumar et al. , 1998Sheline et al. 1996).Despite these observations, the precise nature and extent of the neuroanatomical changes in elderly patients with MDD remains unresolved (Jeste et al. 1988;Morris and Rapoport 1990).Late-life MDD is also consistently associated with medical comorbidity (Berkman et al 1986;Gierz and Jeste 1993;Katz et al. 1994;Katz 1996;Lacro and Jeste 1994;Rodin and Voshart 1986). A broad spectrum of medical disorders including cardiovascular, musculoskeletal, gastrointestinal, pulmonary and metabolic disturbances are associated with both major and minor forms of depression (Alexopolous et al. 1988;Katz et al. 1994;Koenig et al. 1993;Lacro and Jeste 1994). Despite this association, the relationship between neuroanatomical abnormalities and medical disorders in late-life and their relative contributions to the pathophysiology of mood disorders remain unknown.In an earlier report, we demonstrated that the odds of developing MDD in late-life increased with overall medical burden and larger whole brain cerebrospinal fluid (CSF) volume (Kumar et al. 1997a). However, only global measures ...

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“…Moreover, the thin sectioning and staining processes result in tissue shrinkage and geometric distortion, and the staining process is usually time-consuming. Highresolution MRI for non-destructive microscopic analysis of tissue specimens has been proposed [4][5][6]. Although the in-plane resolution of MRI is far inferior to that of light microscopy using histological sections, it has excellent soft tissue contrast (such as T 1 and T 2 ), is non-destructive and can provide true 3D image data [7], making it a valuable tool for the study of fixed tissue specimens.…”

mentioning

confidence: 99%

Ex vivo imaging of mouse brain using micro-CT with non-ionic iodinated contrast agent: a comparison with myelin staining

Saito

Murase²

2012

The British Journal of Radiology

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Objective: We investigated the use of micro-CT with contrast agent for the noninvasive characterisation of fixed mouse brain tissue specimens as a means to differentiate between grey and white matter. Methods: Nine mice were divided into two groups for micro-CT (n56) and myelin staining (n53) experiments. Six mice underwent in vivo micro-CT and were then prepared for brain specimens by transcardiac perfusion with paraformaldehyde. The six mouse brains were soaked in two different concentrations of non-ionic iodinated contrast agents (60 and 150 mg ml 21 ). Immersion times used for each concentration of iodine were for 3, 7 and 14 days. Three-dimensional ex vivo micro-CT images were acquired with a resolution of 39 mm 3 to create isotropic images. The other three mice were stained for evaluation of the myelin structure. Results: Soaking the brains in non-ionic iodinated contrast agent resulted in clear differences in signal between the grey matter, the white matter and the ventricular spaces. The 150 mg ml 21 contrast agent solution yielded images with better contrast-tonoise ratio (CNR) than 60 mg ml 21 iodine contrast agent solution. 14 days of soaking yielded images with better CNR than 3 and 7 days. The CT contrast of grey and white matter derived from the iodine-soaked fixed brains was strongly related to tissue myelin. Conclusion:The present study demonstrated that micro-CT can be used to detect the mouse brain myelin structure at 3, 7 and 14 days after fixation using a CT contrast agent.

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Utility of Specimen Magnetic Resonance Imaging (Mri) in Clinical Neuropathology

Cited by 29 publications

References 0 publications

Postmortem whole-body magnetic resonance imaging as an adjunct to autopsy: Preliminary clinical experience

Postmortem whole-body magnetic resonance imaging as an adjunct to autopsy: Preliminary clinical experience

Atrophy and High Intensity Lesions Complementary Neurobiological Mechanisms in Late-Life Major Depression

Ex vivo imaging of mouse brain using micro-CT with non-ionic iodinated contrast agent: a comparison with myelin staining

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