Utility of the oestrogen‐dependent vaginal candidosis murine model in evaluating the efficacy of various therapies against vaginal <i>Candida albicans</i> infection

Hamad, Mawieh; Muta'eb, Enas; Abu-Shaqra, Qasem; Fraij, Abeer; Abu-Elteen, Khaled H.; Yasin, Salem R.

doi:10.1111/j.1439-0507.2006.01200.x

Cited by 13 publications

(11 citation statements)

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“…Estrogen predisposes to VC by several proposed mechanisms, including the enhancement of the pathogenic potential of Candida species and the suppression of host immunity [7-11]. Induction of a pseudoestrous state by estrogen is routinely used to establish experimental persistent C. albicans vaginal infection in rodents [10,12-14]. Furthermore, estrogen, administered on a weekly basis, can induce persistent VC in naive non-germ-free Balb/c mice [13].…”

mentioning

confidence: 99%

Patterns of Expression of Vaginal T-Cell Activation Markers during Estrogen-Maintained Vaginal Candidiasis

Alsadeq

Hamad

Abu-Elteen

2008

All Asth Clin Immun

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The immunosuppressive activity of estrogen was further investigated by assessing the pattern of expression of CD25, CD28, CD69, and CD152 on vaginal T cells during estrogen-maintained vaginal candidiasis. A precipitous and significant decrease in vaginal fungal burden toward the end of week 3 postinfection was concurrent with a significant increase in vaginal lymphocyte numbers. During this period, the percentage of CD3+, CD3+CD4+, CD152+, and CD28+ vaginal T cells gradually and significantly increased. The percentage of CD3+ and CD3+CD4+ cells increased from 43% and 15% at day 0 to 77% and 40% at day 28 postinfection. Compared with 29% CD152+ vaginal T cells in naive mice, > 70% of vaginal T cells were CD152+ at day 28 postinfection. In conclusion, estrogen-maintained vaginal candidiasis results in postinfection time-dependent changes in the pattern of expression of CD152, CD28, and other T-cell markers, suggesting that T cells are subject to mixed suppression and activation signals.

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confidence: 99%

Patterns of Expression of Vaginal T-Cell Activation Markers during Estrogen-Maintained Vaginal Candidiasis

Alsadeq

Hamad

Abu-Elteen

2008

All Asth Clin Immun

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“…An experimental mouse model of Candida vaginitis has been established and historically used for the past few decades to study mucosal host response to Candida as well as for testing antifungal therapies 3,4,11,13,16,17,19,21,24,25,37 . The protocols presented here incorporate efficient and less labor-intensive methods, and appear to be one of the most optimized model systems of Candida vaginitis described to date.…”

Section: Discussionmentioning

confidence: 99%

“…This model has also been employed to test potential antifungal therapies in vivo 13,24 . The model requires that the animals be maintained in a state of pseudoestrus for optimal Candida colonization/infection 6,14,23 .…”

Section: Introductionmentioning

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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of <em>Candida</em> vaginitis

Yano

Fidel

2011

JoVE

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Vulvovaginal candidiasis (VVC), caused by Candida species, is a fungal infection of the lower female genital tract that affects approximately 75% of otherwise healthy women during their reproductive years 18,[32][33][34] . Predisposing factors include antibiotic usage, uncontrolled diabetes and disturbance in reproductive hormone levels due to pregnancy, oral contraceptives or hormone replacement therapies 33,34 . Recurrent VVC (RVVC), defined as three or more episodes per year, affects a separate 5 to 8% of women with no predisposing factors 33 .An experimental mouse model of VVC has been established and used to study the pathogenesis and mucosal host response to Candida 3,4,11,16,17,19,21,25,37 . This model has also been employed to test potential antifungal therapies in vivo 13,24 . The model requires that the animals be maintained in a state of pseudoestrus for optimal Candida colonization/infection 6,14,23 . Under such conditions, inoculated animals will have detectable vaginal fungal burden for weeks to months. Past studies show an extremely high parallel between the animal model and human infection relative to immunological and physiological properties 3,16,21 . Differences, however, include a lack of Candida as normal vaginal flora and a neutral vaginal pH in the mice.Here, we demonstrate a series of key methods in the mouse vaginitis model that include vaginal inoculation, rapid collection of vaginal specimens, assessment of vaginal fungal burden, and tissue preparations for cellular extraction/isolation. This is followed by representative results for constituents of vaginal lavage fluid, fungal burden, and draining lymph node leukocyte yields. With the use of anesthetics, lavage samples can be collected at multiple time points on the same mice for longitudinal evaluation of infection/colonization. Furthermore, this model requires no immunosuppressive agents to initiate infection, allowing immunological studies under defined host conditions. Finally, the model and each technique introduced here could potentially give rise to use of the methodologies to examine other infectious diseases of the lower female genital tract (bacterial, parasitic, viral) and respective local or systemic host defenses. Video LinkThe video component of this article can be found at

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“…Desta forma, um inóculo contendo cerca de 10 7 UFC/mL (HAMAD et al, 2006) de C. albicans da linhagem ATCC 90028, mantida no LATO, foi preparado para infectar as fêmeas e induzir nestas a vaginite fúngica (VF).…”

Section: 23-inoculação De Candida Albicans No Canal Vaginal Das Fêunclassified

“…No caso específico dos camundongos, estudos mostram que para CV as fêmeas devem ser infectadas durante o estro (FIDEL et al, 2000, CLEMONS et al, 2004HAMAD et al, 2006, fase no camundongo persiste por apenas um dia.…”

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Efeitos da Terapia Fotodinâmica mediada por laser de emissão vermelha e azul de metileno em vaginite induzida por Candida albicans

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Utility of the oestrogen‐dependent vaginal candidosis murine model in evaluating the efficacy of various therapies against vaginal Candida albicans infection

Cited by 13 publications

References 13 publications

Patterns of Expression of Vaginal T-Cell Activation Markers during Estrogen-Maintained Vaginal Candidiasis

Patterns of Expression of Vaginal T-Cell Activation Markers during Estrogen-Maintained Vaginal Candidiasis

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of <em>Candida</em> vaginitis

Efeitos da Terapia Fotodinâmica mediada por laser de emissão vermelha e azul de metileno em vaginite induzida por Candida albicans

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