Utilization of a scientifically operated charge-coupled device detector for high-performance thin-layer chromatographic analysis of tetracyclines

Abstract

A high-performance thin-layer chromatography (HPTLC) method using a scientifically operated charge-coupled device detector is described for the assay of tetracycline pharmaceutical products. Quantitative information can be obtained for all samples on a TLC plate within a few seconds. The separation efficiency and detection limits were determined on both normal phase and reverse phase TLC plates. Fluorescence detection mode offers higher sensitivity than fluorescence quenching mode. The dynamic range, sensitivity, accuracy and precision of the system were evaluated. Detection limits of the impurities are in the range of 0.1 to 0.5 ng or 0.3 to 1% of tetracycline, depending on the compound, with a recovery percentage over 85%. The existing impurities in tetracycline capsules were determined using both HPLC and HPTLC techniques. All of the impurities were below the regulation level.

High performance thin layer chromatography (HPTLC) method development and validation for determination of doxycycline hyclate in capsule and tablet formulations

“…These values are in the same range with the HPLC methods available for Doxycycline [24,25]. TLC Methods developed before for the drug are designed to serve qualitative purposes, hence it is not possible to compare and contrast these methods with the one developed in this method [16,17]. Linearity, range and calibration curves.…”

“…There are various methods for determination of doxycycline; including microbiology [14], thin layer chromatography [15], TLC with densitometry for a binary mixture of doxycycline with ambroxol hydrochloride [16], HPTLC with charge coupled detector device (CCD) [17], infrared spectroscopy [18] and High Performance Liquid Chromatography (HPLC) [4].…”

Estudo da sorção do antibiótico oxitetraciclina a solos e ácidos húmicos e avaliação dos mecanismos de interação envolvidos

“…A Espectroscopia de Fluorescência na Região do UV-Visível é uma técnica largamente utilizada no estudo das SH, e em particular dos AH, já que estes apresentam uma forte característica de emissão da radiação UV-Visível em função da ocorrência de grande número de sistemas aromáticos conjugados em sua estrutura molecular [88,89] . Ela também tem sido empregada para a determinação de tetraciclinas em águas superficiais [90] , pois a ligação π dos anéis conjugados da estrutura molecular funciona como doadora de elétrons o que favorece o fenômeno de emissão de radiação [91] , similar ao caso dos AH. Carvalho Alguns aspectos importantes a serem considerados nesta técnica [94] :…”