Utilization of agro waste pectin for the production of industrially important polygalacturonase

Jahan, Nayyar; Shahid, Faiza; Aman, Afsheen; Mujahid, Talat Yasmeen; Qader, Shah Ali Ul

doi:10.1016/j.heliyon.2017.e00330

Cited by 34 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several agro-industrial waste like wheat bran, rice bran can be effective for production of industrially important enzymes [11]. According to Jahan et al, 2017 [12] wheat bran was found to be the best source production of pectinases. Ten days of incubation period was optimal to yield high enzyme titres (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

Baig¹,

Apastambh²

2017

J Microbiol Biotech Res

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

Baig¹,

Apastambh²

2017

J Microbiol Biotech Res

View full text Add to dashboard Cite

show abstract

“…All were incubated in an incubator at 37 ± 2 °C for 24, 48 and 72 h in static condition. After, the respective fermentation period, the pectinase and amylase activity was determined to find the optimum fermentation period …”

Section: Methodsmentioning

confidence: 99%

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

Viayaraghavan¹,

Kumar²,

Arasu

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND Simultaneous production of commercial enzymes using agro‐industrial residues by statistical approach is an important perspective in an industrial point of view. Despite the advantages of statistical methods optimization, the report on simultaneous production of pectinase and amylases are limited. The accumulation of agro‐industrial residues causes serious environmental problems; however, citrus peel can be the important substrate for various enzymes production, including pectinase. These enzymes involving saccharification process and act as clarifying agent. RESULTS In this study, orange peel and banana peel mixture were used as the suitable substrate for pectinase and amylase production using Bacillus pumilus in solid‐state culture. The process parameters were optimized for simultaneous production of enzymes by a traditional‐one‐variable‐at‐a‐time approach, a two level full factorial design, central composite design and response surface methodology. Among the selected variables, moisture content of the medium, pH and mineral supplement significantly influenced pectinase and amylase production. Pectinase production increased over 3‐fold, whereas, 2‐fold increase on amylase production was achieved after optimization by statistical approach. The purified pectinase exhibited maximal activity at pH 8.0, temperature of 60 °C and the molecular weight was 60 kDa. The purified amylase was highly active at pH 8.0, at 50 °C and the molecular weight was 37 kDa. The enzyme showed activity on fruit pulp in increasing clarity in orange and carrot juice and the saccharification of starch. CONCLUSION Orange peel and banana peel mixture was effective as a solid medium for the simultaneous production of pectinase and amylase by Bacillus pumilus. Also, our statistical approach to optimize the medium components to yield more pectinase and amylase was fruitful and these enzymes showed appreciable results suitable for various applications. © 2018 Society of Chemical Industry

show abstract

“…Employment of agro‐residues as an alternative carbon source in lieu of commercial xylan or pectin substrates, ensures an economically affordable manufacturing of xylanase and pectinase enzymes . Among various agro‐industrial residues, wheat bran has been mainly reported as a suitable carbon source for the production of xylanase and pectinase enzymes . This study encompasses the enhancement in the yield of xylanase and pectinase enzymes from Bacillus pumilus AJK strain along with reduction in fermentation time interval, using the polysaccharides extracted from wheat bran and citrus peel agro‐waste residues.…”

Section: Introductionmentioning

confidence: 99%

“…[22][23][24] Among various agro-industrial residues, wheat bran has been mainly reported as a suitable carbon source for the production of xylanase and pectinase enzymes. 13,[25][26][27][28][29][30] This study encompasses the enhancement in the yield of xylanase and pectinase enzymes from Bacillus pumilus AJK strain along with reduction in fermentation time interval, using the polysaccharides extracted from wheat bran and citrus peel agro-waste residues. Two-stage statistical design approach was adopted for optimizing solid substrate fermentation process parameters.…”

Section: Introductionmentioning

confidence: 99%

A novel and cost‐effective methodology for enhanced production of industrially valuable alkaline xylano‐pectinolytic enzymes cocktail in short solid‐state fermentation cycle

Agrawal

Varghese

Mahajan

2019

Biotechnology Progress

View full text Add to dashboard Cite

The aim of this study was to enhance the production of xylano‐pectinolytic enzymes concurrently and also to reduce the fermentation period. In this study, the effect of agro‐residues extract‐based inoculum on yield and fermentation time of xylano‐pectinolytic enzymes was studied. Microbial inoculum and fermentation media were supplemented with xylan and pectin polysaccharides derived from agro‐based residues. Enzymes production parameters were optimized through two‐stage statistical design approach. Under optimized conditions (temperature 37°C, pH 7.2, K2HPO4 0.22%, MgSO4 0.1%, gram flour 5.6%, substrate: moisture ratio 1:2, inoculum size 20%, agro‐based crude xylan in production media 0.45%, and agro‐based crude xylan–pectin in inoculum 0.13%), nearly 28,255 ± 565 and 9,202 ± 193 IU of xylanase and pectinase, respectively, were obtained per gram of substrate in a time interval of 6 days only. The yield of both xylano‐pectinolytic enzymes was enhanced along with a reduction of nearly 24 h in fermentation time in comparison with control, using polysaccharides extracted from agro‐residues. The activity of different types of pectinase enzymes such as exo‐polymethylgalacturonase (exo‐PMG), endo‐PMG, exo‐polygalacturonase (exo‐PG), endo‐PG, pectin lyase, pectate lyase, and pectin esterase was obtained as 1,601, 12.13, 5637, 24.86, 118.62, 124.32, and 12.56 IU/g, respectively, and was nearly twofold higher than obtained for all seven types in control samples. This is the first report mentioning the methodology for enhanced production of xylano‐pectinolytic enzymes in short solid‐state fermentation cycle using agro‐residues extract‐based inoculum and production media.

show abstract

Utilization of agro waste pectin for the production of industrially important polygalacturonase

Cited by 34 publications

References 26 publications

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

A novel and cost‐effective methodology for enhanced production of industrially valuable alkaline xylano‐pectinolytic enzymes cocktail in short solid‐state fermentation cycle

Contact Info

Product

Resources

About