In this research, the energy use efficiency (EUE) and greenhouse gas emissions (GHG) of cotton cultivation in Beşiri district of Batman province in Turkey were determined. This research was conducted through face-to-face surveys with 64 farms selected by simple random sampling method in the 2018-2019 cultivation season. The energy input (EI) and energy output (EO) in cotton cultivation were calculated as 52,302.62 MJ/ha and 60,341.03 MJ/ha. Energy inputs consist of electricity energy with 19,948.86 MJ/ha(38.14%), chemical fertilizers energy with 14,163.83 MJ/ha (27.08%), diesel fuel energy with 13,218.49 (25.27%), irrigation water energy with 2563.79 MJ/ha(4.90%), machinery energy with 1071.14 MJ/ha(2.05%), chemicals energy with 797.96 MJ/ha (1.53%), seed energy with 291.46 MJ/ha (0.56%) and human labour energy with 247.09 MJ/ha(0.47%), respectively. Total energy inputs in cotton cultivation can be categorized as 68.79% direct, 31.21% indirect, 5.93% renewable and 94.07% non-renewable. EUE, specific energy (SE), energy productivity (EP) and net energy (NE) in cotton cultivation were calculated as 1.15, 10.23 MJ/kg, 0.10 kg /MJ and 8038.41 MJ/ ha, respectively. Total GHG was calculated as 3742.59 kgCO2-eq ha-1 for cotton cultivation with the greatest share taken by nitrogen (26.19%). Nitrogen was followed by electricity (24.73%), irrigation water (18.48%), diesel fuel (17.31%), seed (5.04%), chemicals (2.93%), phosphorous (2.74%), human labour (2.36%), potassium (0.19%) and machinery (0.03%), respectively. GHG ratio value was calculated as 0.73 kgCO2-eq kg-1 in cotton cultivation.