Utilization of coir fibers as an eco-friendly substitute for costly gelling agents for in vitro orchid seed germination

Aggarwal, Simmi; Chongtham, Nirmala

doi:10.1016/j.scienta.2011.10.010

Cited by 16 publications

(14 citation statements)

References 12 publications

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“…Root elongation in vitro is mainly influenced by the substrate and medium [15,16]. Some of the problems with agar as a substrate have already been stated like gel strength, mineral composition, mineral availability, inhibitory compounds that affect the plant growth and development [16][17][18]. Roots of apple and cherry birch produced in gelled medium were thick and had fewer secondary roots, similar to reports on white spruce in agar based medium [19].…”

Section: Resultssupporting

confidence: 71%

Improved in vitro rooting in liquid culture using a two piece scaffold system

Shukla

Piunno

Saxena

et al. 2019

Engineering in Life Sciences

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Plant tissue culture techniques have been used to propagate horticultural crops at a commercial scale for more than three decades. However, due to the high cost it is generally only used for high value crops. To increase production efficiency and make micropropagation viable for a wider range of species, new approaches to address key steps of the process with high labor inputs need to be evaluated. For this study, a two‐piece scaffold system was designed, prototyped using 3D printing, and tested to physically hold plants upright thereby facilitating liquid based rooting. This system was evaluated with Malus domestica, Betula lenta, and Musa sp. using static liquid culture as well as rocker based temporary immersion system and compared to rooting in semi‐solid based medium as is commonly practiced. Significantly, earlier rooting was observed in all three species in liquid when compared to semi‐solid culture system, and plants cultured in liquid on the rocker generally performed better than those in static liquid. In addition to quicker, more uniform rooting, reducing labor requirements, and preventing root damage. This newly designed system is simple, easy to use, will help to improve efficiency, and reduce the cost of micropropagation.

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Section: Resultssupporting

confidence: 71%

Improved in vitro rooting in liquid culture using a two piece scaffold system

Shukla

Piunno

Saxena

et al. 2019

Engineering in Life Sciences

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“…CF and BV were both purchased from the Beijing Kaiyin Organic Fertilizer Production Co. (Beijing, China). CF was air-dried and then cut with a grass-cutting multifunctional shredder (model 9ZF-500B; Qizheng Mechanical Equipment Manufacturing Co., Guangdong, China) to a length of 3 to 5 cm, a length that was reported to optimize media properties (Aggarwal and Nirmala, 2012). BV was diluted in 500 L of water [water hardness £10 d, pH between 6.8 and 7.1, and sodium chloride (NaCl) content <2 mmolÁL -1 ] before it was added to the CGW; 2 mL of BV was added per 100 kg of CGW (dry weight).…”

Section: Selection and Preparation Of Raw Materialsmentioning

confidence: 99%

“…CF is a plant-derived and readily available organic waste material that is obtained from the mesocarp (husk) of coconut; CF is one of the hardest plant fibers known (Aggarwal and Nirmala, 2012). CF also is hydrophilic, and because it is highly porous, it absorbs and retains both water and air (Koch and Barthlott, 2009).…”

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confidence: 99%

“…CF also is hydrophilic, and because it is highly porous, it absorbs and retains both water and air (Koch and Barthlott, 2009). Although water-absorbing polymers are frequently added to peat-based media to increase the absorption of water that is applied to the dry media, these wetting agents are unnecessary in media that contain the appropriate amount of CF (Aggarwal and Nirmala, 2012). The relatively abundant and large pores on the surface of CF particles may explain why addition of CF increases water uptake, drainage, and gas exchange in soilless media (Carlile et al, 2015).…”

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confidence: 99%

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Coir Fiber and Bamboo Vinegar Improve the Quality of Composted Green Waste as a Growing Medium for Peacock Arrowroot

Zhang¹,

Sun²

2017

hortte

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Composting is considered an environmentally sound and economically viable alternative for the management of organic residues. Although compost product may be used as a peat substitute in soilless culture, it generally has poor physical structure, low nutrient content, high pH, and high salt content. This study chose the coir fiber (CF) produced from coconut (Cocos nucifera) and bamboo vinegar (BV) produced from mottled bamboo (Phyllostachys bambusoides) as the media amendments, and evaluated the effects of CF (at 0%, 15%, and 25%) and/or BV (at 0%, 0.5%, and 1.0%) on the physical, chemical, and microbiological properties of composted green waste (CGW) and on the growth of peacock arrowroot (Calathea makoyana). The highest quality growing medium and the best growth of peacock arrowroot were obtained when CGW was amended with the combination of 15% CF and 0.5% BV; the lowest quality medium and the least plant growth were obtained with nonamended CGW. The optimal combination not only improved particle-size distribution and adjusted bulk density (BD), porosity, and water-holding capacity (WHC) into ideal ranges, but it also decreased pH and electrical conductivity (EC) and increased microbial numbers, enzyme activities, and macro- and micronutrient contents in the CGW. Relative to the nonamended CGW, the optimal CGW reduced the BD from 0.58 g·cm−3 to 0.34 g·cm−3 and the pH from 8.05 to 5.61, and increased the total porosity (TPS) from 48.1% to 77.0% and the WHC from 57.4% to 75.5%; the optimal CGW increased shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, plant height, crown breadth, number of leaves, and total root length by 83.9%, 77.8%, 66.1%, 65.1%, 63.6%, 73.8%, 55.6%, and 65.2%, respectively.

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“…Alternative substrates have also been used, in order to enhance both root development and the subsequent acclimatization of the plants in greenhouse conditions (ROCHA et al, 2006;VIEIRA et al, 2007;CASANOVA et al, 2008;DAMIANI;SCHUCH, 2009;KAÇAR et al, 2010;AGGARWAL;NIRMALA, 2012;GALDIANO JUNIOR et al, 2012), and might be useful for promoting in vitro rhizogenesis of H. chrysotrichus. In order to achieve better results, liquid medium-soaked vermiculite, perlite, and polyurethane sponges represent promising alternatives to agar and are also less expensive (BRAGA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

Rabaiolli

Reiniger

Stefanel

et al. 2017

CERNE

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ABSTRACT:The golden trumpet tree Handroanthus chrysotrichus (Bignoniaceae) is an important species, owing to its economic, ecological, medicinal, and ornamental value. However, its production is hindered due to lack of seed availability and viability improved methods of vegetative propagation are needed. Therefore, we evaluated the effect of agar on in vitro rhizogenesis of H. chrysotrichus shoots and the subsequent ex vitro acclimatization of the plants obtained. In the in vitro rhizogenesis assay, we cultured the micropropagated shoots on half-strength nutritive Woody Plant Medium (½WPM) supplemented with 30 g . L -1 sucrose, 50 mg . L -1 myoinositol, 30 cm³ of vermiculite, and one of the three agar concentrations (0, 3.5, or 7 g . L -1 ), and after 30 d, we assessed the shoots for root production and leaf number. Thereafter, the plants were subjected to a 49-d ex vitro acclimatization period, which consisted of two stages in a growth room and one stage in a greenhouse; we assessed plant survival, leaf number and increases in stem height at the end of each stage. We found that the addition of agar to the growth medium was not necessary to induce adventitious rooting, especially with respect to secondary roots, and that plants grown on medium without agar had the highest number of leaves after acclimatization. Therefore, agar actually hindered both in vitro rhizogenesis and ex vitro acclimatization. The ex vitro acclimatization result in high survival and resumption of growth and development of H. chrysotrichus micropropagated plants, and this step is not affected by agar on in vitro rhizogenesis. AGAR NÃO AFETA A RIZOGÊNESE IN VITRO E A ACLIMATIZAÇÃO EX VITRO DE Handroanthus chrysotrichus RESUMO:Handroanthus chrysotrichus (Mart. ex DC.) J. Mattos (Bignoniaceae), conhecida como ipê-amarelo, é uma espécie dotada de importância econômica, ecológica e ornamental. No presente trabalho o objetivo foi avaliar o efeito do ágar na rizogênese in vitro e na subsequente aclimatização ex vitro, bem como a eficiência deste processo em relação à sobrevivência e à retomada do crescimento e desenvolvimento das plantas micropropagadas de H. chrysotrichus. Na rizogênese in vitro, brotações micropropagadas na ausência de reguladores de crescimento foram cultivadas in vitro por 30 dias, em meio nutritivo WPM cujas concentrações de sais foram reduzidas à metade (½WPM), acrescido de 30 g . L -1 de sacarose, 50 mg . L -1 de mio-inositol e 30 cm³ de vermiculita, na presença de diferentes concentrações de ágar (0; 3,5 ou 7 g . L -1 ) e ausência de fitorreguladores. Decorrido esse período, as plantas provenientes dos tratamentos do ensaio de rizogênese foram submetidas à aclimatização ex vitro, processo composto por três etapas e duração de 49 dias. O ágar foi dispensável na indução de raízes adventícias, principalmente no que diz respeito às secundárias. Igualmente, na aclimatização, plantas provenientes de cultivo na ausência de ágar apresentaram melhor desempenho, em especial no que diz respeito ao número de folhas. A ausência de ága...

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Utilization of coir fibers as an eco-friendly substitute for costly gelling agents for in vitro orchid seed germination

Cited by 16 publications

References 12 publications

Improved in vitro rooting in liquid culture using a two piece scaffold system

Improved in vitro rooting in liquid culture using a two piece scaffold system

Coir Fiber and Bamboo Vinegar Improve the Quality of Composted Green Waste as a Growing Medium for Peacock Arrowroot

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

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