Utilization of corncob hydrolysate enables 2,3-butanediol production in Enterobacter cholerae

Abstract

2,3-Butanediol (2,3-BDO) is an important industrial diol that could function in various fields. Currently, there are many substrates used for 2,3-BDO biosynthesis, but studies using green carbon sources such as corncob hydrolysate as a substrate are lacking. As a widely distributed waste lignocellulose-derived substrate, corncob hydrolysate is nutrient-rich and cost-effective. The present study evaluated 2,3-BDO production via an Enterobacter cholerae strain using corncob hydrolysate as carbon source. Chemical component analysis showed that concentrated corncob hydrolysate contained 233 g/L total sugar and showed no inhibitory effect, but it was beneficial for 2,3-BDO synthesis. Optimization experiments for fermentation resulted in a titer of 47.23 g/L 2,3-BDO with a yield of 0.30 g/g and a productivity rate of 0.66 g/L•h. This study is expected to provide insights for large-scale bioproduction of bulk chemicals utilizing corncob hydrolysates.

Metabolic engineering in lignocellulose biorefining for high-value chemicals: recent advances, challenges, and outlook for enabling a bioeconomy

“…(Ramakrishnan et al, 2020). Last but not least, 2,3-butanediol has been produced at titers ranging from 10.30-75.03 g/L using various LCB, with microbial processes involving Klebsiella pneumoniae, K. oxytoca, Pantoea agglomerans, Enterobacter species, and Paenibacillus polymyxa (Cortivo et al, 2019;Hazeena et al, 2019;Pasaye-Anaya et al, 2019;Okonkwo et al, 2021;Rehman et al, 2021;Ma et al, 2022;Li et al, 2023b).…”