Utilization of DBS Within Drug Discovery: A Simple 2D-LC–MS/MS System to Minimize Blood- and Paper-Based Matrix Effects from FTA Elute™ DBS

Clark, Graeme T; Haynes, Julian J

doi:10.4155/bio.11.81

Cited by 15 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LogD calc values suggested that the drug is positively charged at pH ≤ 3 (the amine group is protonated). When separated with RP–LC–MS and a mobile phase at pH = 3, DBS extracts from FTA Elute cards produced a more focused peak with an increased retention time than a hydro‐organic standard (no paper additive in the sample); that elution profile is consistent with the latter ion pairing mechanism (Clark & Haynes, ).…”

Section: Sample Handling and Preparationmentioning

confidence: 92%

See 1 more Smart Citation

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

226

228

View full text Add to dashboard Cite

Dried blood spots (DBS) typically consist in the deposition of small volumes of capillary blood onto dedicated paper cards. Comparatively to whole blood or plasma samples, their benefits rely in the fact that sample collection is easier and that logistic aspects related to sample storage and shipment can be relatively limited, respectively, without the need of a refrigerator or dry ice. Originally, this approach has been developed in the sixties to support the analysis of phenylalanine for the detection of phenylketonuria in newborns using bacterial inhibition test. In the nineties tandem mass spectrometry was established as the detection technique for phenylalanine and tyrosine. DBS became rapidly recognized for their clinical value: they were widely implemented in pediatric settings with mass spectrometric detection, and were closely associated to the debut of newborn screening (NBS) programs, as a part of public health policies. Since then, sample collection on paper cards has been explored with various analytical techniques in other areas more or less successfully regarding large‐scale applications. Moreover, in the last 5 years a regain of interest for DBS was observed and originated from the bioanalytical community to support drug development (e.g., PK studies) or therapeutic drug monitoring mainly. Those recent applications were essentially driven by improved sensitivity of triple quadrupole mass spectrometers. This review presents an overall view of all instrumental and methodological developments for DBS analysis with mass spectrometric detection, with and without separation techniques. A general introduction to DBS will describe their advantages and historical aspects of their emergence. A second section will focus on blood collection, with a strong emphasis on specific parameters that can impact quantitative analysis, including chromatographic effects, hematocrit effects, blood effects, and analyte stability. A third part of the review is dedicated to sample preparation and will consider off‐line and on‐line extractions; in particular, instrumental designs that have been developed so far for DBS extraction will be detailed. Flow injection analysis and applications will be discussed in section IV. The application of surface analysis mass spectrometry (DESI, paper spray, DART, APTDCI, MALDI, LDTD‐APCI, and ICP) to DBS is described in section V, while applications based on separation techniques (e.g., liquid or gas chromatography) are presented in section VI. To conclude this review, the current status of DBS analysis is summarized, and future perspectives are provided. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:361–438, 2016.

show abstract

Section: Sample Handling and Preparationmentioning

confidence: 92%

“…Investigation of matrix effects for a panel of ten drugs (from FTA Elute cards, with MeOH for SLE) showed that they largely depended on the compounds, and both ionization suppression and enhancement were observed (Clark & Haynes, ).…”

Section: Sample Handling and Preparationmentioning

confidence: 99%

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

226

228

View full text Add to dashboard Cite

show abstract

“…During the SPE optimization, DBS samples were prepared using chloroquine, desipramine and midazolam (50 ng/ml each) in rat blood. DMPK-C cards were selected for the DBS experiments as they are chemically untreated since cards impregnated with chemicals have been previously reported to interfere with the analyte signals [46].…”

Section: On-line Dbs-spe Methods Developmentmentioning

confidence: 99%

Automated Direct Extraction and Analysis of Dried Blood Spots Employing On-Line SPE High-Resolution Accurate Mass Bioanalysis

2014

View full text Add to dashboard Cite

show abstract

“…Paper‐based analytical devices (PADs) have shown great promise as point‐of‐care testing (POCT) platforms due to their advantages of being user‐friendly, rapid, robust, affordable, and scalable for manufacturing. Achieving efficient POCT within PADs has therefore found widespread applications, especially at home and in the field, such as clinical prognostics and diagnostics (Hu et al, ; Y. Wang, Li, Kong, & Sun, ), drug discovery (Clark & Haynes, ), genetic and forensic study (Hart et al, ), environmental monitoring (Peng, Zhang, Soeller, & Travas‐Sejdic, ), food safety analysis (Dzantiev, Byzova, Urusov, & Zherdev, ). For paper‐based POCT, cost‐effective, facile and sensitive detection rely heavily on the design and construction of probes.…”

Section: Introductionmentioning

confidence: 99%

Paper‐based point‐of‐care test with xeno nucleic acid probes

Xiao

Jin

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Bridging the unmet need of efficient point-of-care testing (POCT) in biomedical engineering research and practice with the emerging development in artificial synthetic xeno nucleic acids (XNAs), this review summarized the recent development in paperbased POCT using XNAs as sensing probes. Alongside the signal transducing mode and immobilization methods of XNA probes, a detailed evaluation of probe performance was disclosed. With these new aspects, both researchers in synthetic chemistry / biomedical engineering and physicians in clinical practice could gain new insights in designing, manufacturing and choosing suitable reagents and techniques for POCT. K E Y W O R D S aptamer, chemical modification, paper-based analytical devices (PADs), point-of-care testing (POCT), xeno nucleic acids (XNAs)

show abstract

Utilization of DBS Within Drug Discovery: A Simple 2D-LC–MS/MS System to Minimize Blood- and Paper-Based Matrix Effects from FTA Elute™ DBS

Abstract: A generic 2D-LC-MS/MS system has been developed that eliminates paper-based matrix effects and eliminates or controls dried blood spot matrix effects for a panel of compounds extracted from FTA Elute™ with methanol.

Cited by 15 publications

References 26 publications

The use of mass spectrometry to analyze dried blood spots

The use of mass spectrometry to analyze dried blood spots

Automated Direct Extraction and Analysis of Dried Blood Spots Employing On-Line SPE High-Resolution Accurate Mass Bioanalysis

Paper‐based point‐of‐care test with xeno nucleic acid probes

Contact Info

Product

Resources

About