Utilization of erythrosine B in spectrofluorimetric quenching analysis of amlodipine and perindopril in pharmaceutical and biological matrices

Abstract

A spectrofluorimetric approach that is sensitive, simple, validated, and cost‐effective has been proposed for the estimation of amlodipine (AML) and perindopril (PER) in their bulk powders, pharmaceutical formulations, and spiked human plasma. The recommended approach utilized the quantitative quenching effect of the two cited drugs on the fluorescence intensity of erythrosine B, as a result of complex binary reactions among each drug with erythrosine B at pH 3.5 (Teorell and Stenhagen buffer). The quenching of erythrosine B fluorescence was recorded at 554 nm after excitation at 527 nm. The calibration curve was detected in the range 0.25–3.0 μg ml−1, with a correlation coefficient of 0.9996 for AML, and 0.1–1.5 μg ml−1, with a correlation coefficient of 0.9996 for PER. The established spectrofluorimetric approach was validated for the estimation of the cited drugs with high sensitivity regarding International Council on Harmonization guidelines. Therefore, the established approach could be utilized for quality control of the cited drugs in their pharmaceutical formulations.

A facile on–off fluorescence approach for fluvoxamine determination in pharmaceutical tablets; application to content uniformity testing

“…On the hand, using erythrosine B as Spectrouorimetric probe was frequently employed for the purpose of quantifying a variety of compounds with high sensitivity. 27,28 Therefore, in the present study, it was utilized for the determination of FLX. The reaction relies on forming an association complex between the dye and the investigated compound.…”

Design of resonance Rayleigh scattering and spectrofluorimetric methods for the determination of the antihistaminic drug, hydroxyzine, based on its interaction with 2,4,5,7‐tetraiodofluorescein: Evaluation of analytical eco‐scale and greenness/whiteness algorithms

“…Neither the fluorometric methodology nor the RRS methodology has yet been used for the quantitation of HXZ. The reaction ion pair formation between certain drugs (contain basic amine center) and erythrosin B or 2,4,5,7-tetraiodofluorescein (TDF) has been widely utilized to determine a variety of active pharmaceutical ingredients such as terbinafine, rupatadine, dapoxetine naftidrofuryl, duloxetine, amlodipine, perindopril, atomoxetine, fluvoxamine, and venlafaxine by applying either spectrofluorimetric technique or RRS technique [16][17][18][19][20][21][22][23][24]. Furthermore, TDF possesses advantageous characteristics in terms of its cost-effectiveness and long-term stability.…”