Utilization of G‐Quadruplex‐Forming Aptamers for the Construction of Luminescence Sensing Platforms

Ma, Dik‐Lung; Wang, Wanhe; Mao, Zhifeng; Kang, Tian-Shu; Han, Quan‐Bin; Chan, Philip Wai Hong; Leung, Chung‐Hang

doi:10.1002/cplu.201600036

Cited by 37 publications

(16 citation statements)

References 80 publications

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“…Their involvement in important biological functions, such as telomere maintenance [ 1 , 2 , 3 ], DNA-protein recognition [ 4 , 5 ] and protein inhibition [ 6 , 7 ] has been supposed. Moreover, the stability of Qs in a large variety of experimental conditions has encouraged their exploitation in biotechnology, affording a reliable scaffold for biosensors and nanomechanical devices [ 8 , 9 , 10 ] where they can also form hybrid structures with novel two-dimensional materials [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides

Imperatore

Varriale

Rivieccio

et al. 2020

IJMS

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The synthesis of two 5′-end (4-dimethylamino)azobenzene conjugated G-quadruplex forming aptamers, the thrombin binding aptamer (TBA) and the HIV-1 integrase aptamer (T30695), was performed. Their structural behavior was investigated by means of UV, CD, fluorescence spectroscopy, and gel electrophoresis techniques in K+-containing buffers and water-ethanol blends. Particularly, we observed that the presence of the 5′-(4-dimethylamino)azobenzene moiety leads TBA to form multimers instead of the typical monomolecular chair-like G-quadruplex and almost hampers T30695 G-quadruplex monomers to dimerize. Fluorescence studies evidenced that both the conjugated G-quadruplexes possess unique fluorescence features when excited at wavelengths corresponding to the UV absorption of the conjugated moiety. Furthermore, a preliminary investigation of the trans-cis conversion of the dye incorporated at the 5′-end of TBA and T30695 showed that, unlike the free dye, in K+-containing water-ethanol-triethylamine blend the trans-to-cis conversion was almost undetectable by means of a standard UV spectrophotometer.

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Section: Introductionmentioning

confidence: 99%

Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides

Imperatore

Varriale

Rivieccio

et al. 2020

IJMS

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“…[1][2][3] The remarkable variety of topologies as displayed by these four-stranded DNA structures [4,5] makes them promising tools for various technological applications. [6,7] G-quadruplexes can be composed of one (monomolecular), two (bimolecular), three (trimolecular), or four (tetramolecular) strands. Depending on the relative strand orientation as fixed by the type of loops in case of monomolecular G4s, topologies can be of a parallel, antiparallel, or hybrid type.…”

Section: Introductionmentioning

confidence: 99%

Sugar Puckering Drives G‐Quadruplex Refolding: Implications for V‐Shaped Loops

Haase

Dickerhoff

Weisz

2019

Chemistry A European J

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A DNA G‐quadruplex adopting a (3+1) hybrid structure was modified in two adjacent syn positions of the antiparallel strand with anti‐favoring 2′‐deoxy‐2′‐fluoro‐riboguanosine (FrG) analogues. The two substitutions promoted a structural rearrangement to a topology with the 5′‐terminal G residue located in the central tetrad and the two modified residues linked by a V‐shaped zero‐nucleotide loop. Strikingly, whereas a sugar pucker in the preferred north domain is found for both modified nucleotides, the FrG analogue preceding the V‐loop is forced to adopt the unfavored syn conformation in the new quadruplex fold. Apparently, a preferred C3′‐endo sugar pucker within the V‐loop architecture outweighs the propensity of the FrG analogue to adopt an anti glycosidic conformation. Refolding into a V‐loop topology is likewise observed for a sequence modified at corresponding positions with two riboguanosine substitutions. In contrast, 2′‐F‐arabinoguanosine analogues with their favored south‐east sugar conformation do not support formation of the V‐loop topology. Examination of known G‐quadruplexes with a V‐shaped loop highlights the critical role of the sugar conformation for this distinct structural motif.

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“…Betaine functions as a molecular barrier to lower the association rate constant, K 1 , for hybridization and lowers the hybridization efficiency. 45 These additives have been shown in the past to enhance amplication of long PCR products, which reduces the likelihood of intramolecular stem loop formation by singlestranded templates during amplication. 46 To obtain a reliable assay, the catalytic activities of the four sets of probes were investigated and compared under the same conditions.…”

Section: The Formation Of G4-hemin Dnazyme For the Detection Of Denv mentioning

confidence: 99%

An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

et al. 2020

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Utilization of G‐Quadruplex‐Forming Aptamers for the Construction of Luminescence Sensing Platforms

Cited by 37 publications

References 80 publications

Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides

Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides

Sugar Puckering Drives G‐Quadruplex Refolding: Implications for V‐Shaped Loops

An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

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