Utilization of glycoprotein-derived N-acetylglucosamine-L-asparagine during Enterococcus faecalis infection depends on catabolic and transport enzymes of the glycosylasparaginase locus

Combret, Victor; Rincé, Isabelle; Budin-Verneuil, Aurélie; Muller, Cécile; Deutscher, Josef; Hartke, Axel; Sauvageot, Nicolas

doi:10.1016/j.resmic.2023.104169

Cited by 2 publications

References 52 publications

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Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis

Snell,

Manias,

Elbehery

et al. 2024

FEMS Microbes

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Enterococcus faecalis is a commensal bacterium in the gastrointestinal tract (GIT) of humans and other organisms. E. faecalis also causes infections in root canals, wounds, the urinary tract, and on heart valves. E. faecalis metabolizes arginine through the arginine deiminase (ADI) pathway, which converts arginine to ornithine and releases ATP, ammonia, and CO2. E. faecalis arginine metabolism also affects virulence of other pathogens during co-culture. E. faecalis may encounter elevated levels of arginine in the GIT or the oral cavity, where arginine is used as a dental therapeutic. Little is known about how E. faecalis responds to growth in arginine in the absence of other bacteria. To address this, we used RNAseq and additional assays to measure growth, gene expression, and biofilm formation in E. faecalis OG1RF grown in arginine. We demonstrate that arginine decreases E. faecalis biofilm production and causes widespread differential expression of genes related to metabolism, quorum sensing, and polysaccharide synthesis. Growth in arginine also increases aggregation of E. faecalis and promotes decreased susceptibility to the antibiotics ampicillin and ceftriaxone. This work provides a platform for understanding of how the presence of arginine in biological niches affects E. faecalis physiology and virulence of surrounding microbes.

show abstract

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis

Snell,

Manias,

Elbehery

et al. 2024

FEMS Microbes

View full text Add to dashboard Cite

show abstract

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility inEnterococcus faecalis

Snell,

Manias,

Elbehery

et al. 2024

Preprint

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Enterococcus faecalisis a commensal bacterium in the gastrointestinal tract (GIT) of humans and other organisms.E. faecalisalso causes infections in root canals, wounds, the urinary tract, and on heart valves.E. faecalismetabolizes arginine through the arginine deiminase (ADI) pathway, which converts arginine to ornithine and releases ATP, ammonia, and CO2.E. faecalisarginine metabolism also affects virulence of other pathogens during co-culture.E. faecalismay encounter elevated levels of arginine in the GIT or the oral cavity, where arginine is used as a dental therapeutic. Little is known about howE. faecalisresponds to growth in arginine in the absence of other bacteria. To address this, we used RNAseq and additional assays to measure growth, gene expression, and biofilm formation inE. faecalisOG1RF grown in arginine. We demonstrate that arginine decreasesE. faecalisbiofilm production and causes widespread differential expression of genes related to metabolism, quorum sensing, and polysaccharide synthesis. Growth in arginine also increases aggregation ofE. faecalisand promotes decreased susceptibility to the antibiotics ampicillin and ceftriaxone. This work provides a platform for understanding of how the presence of arginine in biological niches affectsE. faecalisphysiology and virulence of surrounding microbes.

show abstract

Utilization of glycoprotein-derived N-acetylglucosamine-L-asparagine during Enterococcus faecalis infection depends on catabolic and transport enzymes of the glycosylasparaginase locus

Cited by 2 publications

References 52 publications

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility inEnterococcus faecalis

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