Utilization of High-Fructose Corn Syrup for Biomass Production Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Aurantiochytrium sp. YLH70

Abstract

High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l(-1) reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l(-1), respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l(-1)day(-1) and 0.11 g g(-1). The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4% of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.

Physicochemical Properties and Storage Stability of Microencapsulated DHA-Rich Oil with Different Wall Materials

“…is a valuable fatty acid for functional foods because it contains a considerable amount of omega-3 polyunsaturated fatty acids (PUFAs). DHA is an important omega-3 polyunsaturated fatty acid that has beneficial effects on human health, such as reducing blood viscosity and preventing cardiovascular diseases, cancer, schizophrenia, and Alzheimer's disease [1][2][3]. Moreover, DHA has an important function in infant brain and retinal development, and it is an essential nutrient for marine fish in the aquaculture industry [4,5].…”

Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production

“…It is worth noting that CSP and glycerol seem to be suitable alternatives to replace the commonly used glutamate and glucose, since they not only allowed a lipid yield increase, but also enhanced DHA production. Yu et al [43] also reported high-fructose corn syrup as a possible option for Aurantiochytrium sp. DHA fermentation, reaching final concentration values of up to 78.5 g/L DW and 51 g/L lipid yields, with a DHA content of 46.4%.…”

“…Regarding alternative and more sustainable N sources, corn steep powder displayed a greater potential for the improved growth of Aurantiochytrium sp., when compared to cheese whey and MNBIO. High fructose corn syrup or corn steep liquor/powder (CSP) is a co-product of corn wet milling process, a well-reported agro-waste often used as a sugar substitute, due to its low cost and high solubility [43]. Apart from its elevated fermentable sugar content, it contains between 35%-45% of protein, being both an N and C source.…”

Isolation of High Carotenoid-producing <i>Aurantiochytrium</i> sp. Mutants and Improvement of Astaxanthin Productivity Using Metabolic Information

“…We previously constructed a lipid fermentation system for Aurantiochytrium cells using waste syrup 27 and brown algae 25 as culture substrates. The main component of waste syrup is fructose, which is a good carbon source for Aurantiochytrium cells.…”

“…Monosaccharides, including glucose, galactose, and fructose; fatty acids; and glycerol are able to support the growth of Aurantiochytrium strains 23 25 . Biodiesel waste water 26 and high-fructose corn syrup 27 can also be utilized. We also reported the use of biomass, including shochu waste water 28 , waste syrup of canned fruits 29 , and brown macroalgae 25 .…”