Utilization of IncP-1 Plasmids as Vectors for Transposon Mutagenesis in Myxobacteria

Saulnier, Patrick; Hanquier, José; Jaoua, Samir; Reichenbach, Hans; Guespin‐Michel, Janine

doi:10.1099/00221287-134-11-2889

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Tn 5 and its derivatives have been used to mutagenize M. xanthus and to identify and clone a number of genes (Kroos & Kaiser, 1984; Kroos et al, 1986; Kuner & Kaiser, 1981). Transposition of Tn 5 into the genome occurs after conjugative transfer of the suicide plasmid pSUP2021 from E. coli to M. xanthus (Saulnier et al, 1988). For the saframycin production strain DM504/15, this technique yielded kanamycin- and streptomycin- resistant mutants at a frequency of about 1 × 10 −5 per recipient cell.…”

Section: Resultsmentioning

confidence: 99%

A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase

et al. 1995

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The gene cluster for the biosynthesis of the heterocyclic quinone antibiotic saframycin Mxl of Myxococcus xanthus DM5W15 was inactivated and tagged by TnS insertions. The tagged genes were cloned in Escherichia coli and used to select overlapping cosmid clones spanning 58 kb of the M. xanthus genome.Gene disruption experiments defined a 2 18 kb contiguous DNA region involved in saf ramycin biosynthesis. Sequencing of part of this region revealed a large ORF containing two 600-amino-acid domains with similarity to peptide synthetase aminolacid-activating sequences, suggesting that saframycin M x l is synthesized by a nonribosomal multienzyme complex, similar to other bioactive peptides.

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Section: Resultsmentioning

confidence: 99%

A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase

et al. 1995

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“…Multicopy Single-Stranded DNA Endogenous plasmids have not been found in M. xanthus, and plasmids from other gram-negative organisms, including broad-host-range plasmids in the IncP group, fail to replicate in this species (14,221). However, M. xanthus does produce an unusual satellite DNA known as multicopy singlestranded DNA (msDNA) (for a review, see Lampson et al, submitted).…”

Section: Analysis Of the Genomementioning

confidence: 99%

Social and developmental biology of the myxobacteria.

Shimkets

1990

Microbiological Reviews

211

120

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“…While some bacteria contain two chromosomes, all myxobacteria contain single chromosomes and were thought not to harbour plasmids until recently. Plasmids could be introduced into M. xanthus but could not be maintained without integrating into the chromosome by homologous recombination, or by integration into a temperate phage attB locus [ 71 , 72 ]. The first autonomously replicating myxobacterial plasmid, pMF1, was discovered in M. fulvus strain 124B02 [ 73 ].…”

Section: Myxobacterial Genome Biologymentioning

confidence: 99%

Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related ‘Omics Studies

2021

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Myxobacteria are fascinating and complex microbes. They prey upon other members of the soil microbiome by secreting antimicrobial proteins and metabolites, and will undergo multicellular development if starved. The genome sequence of the model myxobacterium Myxococcus xanthus DK1622 was published in 2006 and 15 years later, 163 myxobacterial genome sequences have now been made public. This explosion in genomic data has enabled comparative genomics analyses to be performed across the taxon, providing important insights into myxobacterial gene conservation and evolution. The availability of myxobacterial genome sequences has allowed system-wide functional genomic investigations into entire classes of genes. It has also enabled post-genomic technologies to be applied to myxobacteria, including transcriptome analyses (microarrays and RNA-seq), proteome studies (gel-based and gel-free), investigations into protein–DNA interactions (ChIP-seq) and metabolism. Here, we review myxobacterial genome sequencing, and summarise the insights into myxobacterial biology that have emerged as a result. We also outline the application of functional genomics and post-genomic approaches in myxobacterial research, highlighting important findings to emerge from seminal studies. The review also provides a comprehensive guide to the genomic datasets available in mid-2021 for myxobacteria (including 24 genomes that we have sequenced and which are described here for the first time).

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Utilization of IncP-1 Plasmids as Vectors for Transposon Mutagenesis in Myxobacteria

Cited by 6 publications

References 23 publications

A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase

A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase

Social and developmental biology of the myxobacteria.

Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related ‘Omics Studies

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