Utilization of inorganic carbon by marine microalgae

Burns, Bruce D.; Beardall, John

doi:10.1016/0022-0981(87)90125-0

Cited by 181 publications

(132 citation statements)

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“…Points represent the average of 3 replicate readmgs surement of the spatial and temporal variation in pH of a single cell and the rapid change in pH, in response to external conditions. Cytoplasmic pH in the high-calcifying cells of Emiliania huxleyi (Table 1) is more acidic than that reported for other plant cells (Smith 1979, Raven 1980 and other non-calcifying marine microalgae (Raven & Smith 1980, Burns & Beardall 1987 but is similar to values found for the low-calcifying cells (Dixon et al 1989) with a pH, near neutrality. This suggests that the optimum pH, for cellular metabolic pathways in E. huxleyi may be different from some other algae.…”

Section: Discussionsupporting

confidence: 66%

Carbon dioxide availability, intracellular pH and growth rate of the coccolithophore Emiliania huxleyi

Nimer¹,

Brownlee²,

Merrett³

1994

Mar. Ecol. Prog. Ser.

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A 30 "/o reduction in cell number and final cell yield occurred at pH 7.8 increasing to almost 60°0 at pH 7.0; pH, decreased at more acidic external pH down to 6.38 at pH 7.0. Carbon dioxide concentration and external pH appear to be equally important in the growth of high-calcifying cells. The significance of these results is considered in relation to the development of mesoscale blooms of E. huxleyi.

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Section: Discussionsupporting

confidence: 66%

Carbon dioxide availability, intracellular pH and growth rate of the coccolithophore Emiliania huxleyi

Nimer¹,

Brownlee²,

Merrett³

1994

Mar. Ecol. Prog. Ser.

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“…It is thought to be due to the ability of these organisms to actively accumulate inorganic carbon, elevating internal CO2 relative to O2 and thereby suppressing the oxygenase activity of Rubisco and hence photorespiration (Badger et al 1978). This CO,-concentrating mechanism has also been shown to occur in diatoms (Burns and Beardall 1987;Colman and Rotatore 1988). The significance of the Mehler reaction during steady state photosynthesis is much more controversial (Sueltemeyer et al 1986;Brechignac and Furbank 1987).…”

Section: Discussionmentioning

confidence: 92%

Respiratory losses in the light in a marine diatom: Measurements by short‐term mass spectrometry

Weger

Herzig

Falkowski

et al. 1989

Limnology & Oceanography

125

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Mass spectrometric analysis of gas exchange by the diatom Thalassiosira weisflogii grown under at the end of scotophase. When cells were poised at the DIC (dissolved inorganic carbon) compensation point in the light (DIC concentration where net photosynthesis equals zero), mitochondrial 0, consumption was only slightly higher than in the dark. Adding DIC to cells at the compensation point resulted in a rapid increase in both photosynthetic 0, evolution and mitochondrial 0, consumption, indicating that the higher mitochondrial respiration rates observed in the light are probably due to an increase in substrate supply from photosynthesis. If estimates of primary production are based on oxygen exchange, this effect is accounted for; however if radiocarbon is used to measure production, especially in short-term measurements, net production may be significantly overestimated.Phytoplankton respiration is used in aquatic ecology to quantify at least three fundamental parameters. First, knowledge of the rate of respiratory losses is required ' To whom reprint requests should be addressed.

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“…During the same time, pH oscillated regularly between 8.5 (at 0800 h) and 8.2 (at 2000 h) in HL and 8.4 and 8.1 in LL. Although the mechanisms of CO, vs. DIC assimilation are not yet completely understood, Burns and Beardall (1987) have shown that, in the CO, range of variations mentioned above, D. tertiolecta can increase its cell DIC concentration by a factor of 6.9. This CO,-concentrating mechanism [CCM, see review by Beardall and Raven (1990)] can also be induced in N-deficient medium as shown by Beardall et al (1982, 199 1) for Chlorella emersonii.…”

Section: Resultsmentioning

confidence: 99%

Growth‐compensating phenomena in continuous cultures of Dunaliella tertiolecta limited simultaneously by light and nitrate

Sciandra

Gostan

Collos

et al. 1997

Limnology & Oceanography

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Two nitrogen-limited continuous cultures of Dunaliella tertiolecta were grown on light/dark cycles. One was submitted to limiting photon flux density (PFD) and the other to nonlimiting PFD. The growth rate was identical in the two cultures despite the difference in the PFD conditions. Once equilibria were reached in both cultures, the PFD were reversed to simulate a decrease and an increase in light conditions. A large suite of variables was measured to characterize the response of the cells, mainly through the interactions of carbon and nitrogen assimilation pathways. A decrease in irradiance led to a rapid decrease in algal biovolume; the biovolume-based growth rate (p,) descended to levels lower than those estimated for cells of the light-limited culture. An increase in irradiance rapidly led to an increase in lo, which attained values greater than those observed in high-light cells of the other culture before the shift, For the two cultures before and after the shift, cell carbon and cell volume were strongly correlated, showing the same pattern of diel variations.The specific C fixation rates (~(2) of the two cultures before and after the light shift declined significantly during the light periods. Before the light shift, p,C was paradoxically higher in the low light culture and could not be predicted only from the light levels. This suggests that some compensatory phenomena may occur during light and nitrogen limitations, Time variations of cell Chl a due to photoacclimation in both cultures were correlated with their N status. Similarly, the regulation of carboxylase activities (Rubisco) by the light levels was sensitive to the degree of N limitation. We found that nitrogen limitation has an overriding effect compared to light for the regulation of cell volume, C fixation and respiration rates, Chl a synthesis, electron transport system, and Rubisco activities. In cultures subjected to low irradiance, NO,-uptake rate decreased several hours into the dark phase, suggesting a time lag between the end of photosynthate production and the exhaustion of compounds necessary for dissolved inorganic nitrogen assimilation. The implications of these results concerning phytoplankton growth modeling in a variable environment are important because there are no existing models that correctly integrate the simultaneous effects of light and nitrogen on primary production. It is shown that the effects of these limitations were not additive in the range of light and N limitations tested in this experiment.It is now evident that the understanding of physiological processes involved in the acclimation of phytoplanktonic organisms subjected to a variable light or nutrient environment AcknowledgmentsWe thank Gilbert Malara for technical help, Michele Etienne and Olivier Bernard for assistance in programming, Serge Dallot for the statistical analysis, Jean-Francois Berthon for the P vs. I curves, Valerie Martin for the electron transport system analysis, and Patrick Chang for improving the English. The manuscript was imp...

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Utilization of inorganic carbon by marine microalgae

Cited by 181 publications

References 40 publications

Carbon dioxide availability, intracellular pH and growth rate of the coccolithophore Emiliania huxleyi

Carbon dioxide availability, intracellular pH and growth rate of the coccolithophore Emiliania huxleyi

Respiratory losses in the light in a marine diatom: Measurements by short‐term mass spectrometry

Growth‐compensating phenomena in continuous cultures of Dunaliella tertiolecta limited simultaneously by light and nitrate

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