Utilization of mixtures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment

Dean-Ross, Deborah

doi:10.1016/s0168-6496(02)00198-8

Cited by 32 publications

(35 citation statements)

References 27 publications

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“…These strains were chosen based on an initial evaluation of 17 members of the group actinomycetes for their potential for surfactant production and degradation of aromatic compounds (data not shown). Special emphasis was placed on members of the genera Gordonia and Rhodococcus, since they are often found at polluted sites, especially oil-and PAH-contaminated soils (Dean-Roos et al 2002;Lin et al 2005). They also show high cell hydrophobicity and release surface-active substances (Lang & Philp 1998;Nazina et al 2003) and can degrade several organic compounds by mechanisms that may be of interest in PAH degradation.…”

Section: Resultsmentioning

confidence: 99%

Characterization of selected actinomycetes degrading polyaromatic hydrocarbons in liquid culture and spiked soil

Pizzul

Castillo

Stenström

2006

World J Microbiol Biotechnol

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Five strains of the Rhodococcus and Gordonia genera were evaluated for their potential use in bioremediation of polycyclic aromatic hydrocarbons (PAH) with or without another substrate (co-substrate). Their ability to produce biosurfactants or to degrade phenanthrene when growing on glucose, hexadecane and rapeseed oil was tested in liquid medium at 30°C. All strains showed biosurfactant activity. The highest reduction in surface tension was recorded in whole cultures of Rhodococcus sp. DSM 44126 (23.1%) and R. erythropolis DSM 1069 (21.1%) grown on hexadecane and Gordonia sp. APB (20.4%) and R. erythropolis TA57 (18.2%) grown on rapeseed oil. Cultures of Gordonia sp. APB and G. rubripertincta formed emulsions when grown on rapeseed oil. After 14 days of incubation, Rhodococcus sp. DSM 44126 degraded phenanthrene (initial concentration 100 lg ml )1 ) as sole carbon source (79.4%) and in the presence of hexadecane (80.6%), rapeseed oil (96.8%) and glucose (below the limit of detection). The other strains degraded less than 20%, and then with a co-substrate only. Rhodococcus sp. DSM 44126 was selected and its performance evaluated in soil spiked with a mixture of PAH (200 mg kg )1 ). The effect of the addition of 0, 0.1 and 1% rapeseed oil as co-substrate was also tested. Inoculation enhanced the degradation of phenanthrene (55.7% and 95.2% with 0.1% oil and without oil respectively) and of anthracene (29.2% with 0.1% oil). Approximately 96% of anthracene and 62% of benzo(a)pyrene disappeared from the soil (inoculated and control) after 14 days and anthraquinone was detected as a metabolite. Rhodococcus sp. DSM 44126 was identified as Rhodococcus wratislaviensis by 16S rRNA sequencing and was able to degrade anthracene as sole carbon source in liquid culture.

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Section: Resultsmentioning

confidence: 99%

Characterization of selected actinomycetes degrading polyaromatic hydrocarbons in liquid culture and spiked soil

Pizzul

Castillo

Stenström

2006

World J Microbiol Biotechnol

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“…Substrate interactions for PAH mixtures (e.g. competitive inhibition and cometabolism) have often been observed (Walter et al 1991;Bouchez et al 1999;Dean-Ross et al 2002) and they are difficult to predict (Guha et al 1999). These interactions may play an important role in the degradation of PAH in contaminated soils, where the compounds are usually present in mixtures.…”

Section: Characterisation Of Mycobacterium Lp1mentioning

confidence: 99%

Degradation of polycyclic aromatic hydrocarbons in soil by a two-step sequential treatment

et al. 2007

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The objectives of this work were to isolate the microorganisms responsible for a previously observed degradation of polycyclic aromatic hydrocarbons (PAH) in soil and to test a method for cleaning a PAH-contaminated soil. An efficient PAH degrader was isolated from an agricultural soil and designated as Mycobacterium LP1. In liquid culture, it degraded phenanthrene (58%), pyrene (24%), anthracene (21%) and benzo(a)pyrene (10%) present in mixture (initial concentration 50 microg ml(-1) each) and phenanthrene (92%) and pyrene (94%) as sole carbon sources after 14 days of incubation at 30 degrees C. In soil, Mycobacterium LP1 mineralised (14)C-phenanthrene (45%) and (14)C-pyrene (65%) after 10 days. The good ability of this Mycobacterium was combined with the benzo(a)pyrene oxidation effect obtained by 1% w/w rapeseed oil in a sequential treatment of a PAH-spiked soil (total PAH concentration 200 mg kg(-1)). The first step was incubation with the bacterium for 12 days and the second step was the addition of the rapeseed oil after this time and a further incubation of 22 days. Phenanthrene (99%), pyrene (95%) and anthracene (99%) were mainly degraded in the first 12 days and a total of 85% of benzo(a)pyrene was transformed during the whole process. The feasibility of the method is discussed.

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“…Many bacterial species capable of PAH degradation have been isolated and belong to the genera of Arthrobacter (Samanta et al 1999), Pseudomonas (Balashova et al 1999), Rhodococcus (Dean-Ross et al 2002), Mycobacterium (Dean-Ross et al 2002;Kim et al 2005;Moody et al 2001), Sphingomonas (Pinyakong et al 2000;van Herwijnen et al 2003), and Stenotrophomonas (Juhasz et al 2000). In addition, Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

Degradation of phenanthrene by Burkholderia sp. C3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleavage of naphthalene-1,2-diol

Seo

Keum

et al. 2006

Biodegradation

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Burkholderia sp. C3 was isolated from a polycyclic aromatic hydrocarbon (PAH)-contaminated site in Hilo, Hawaii, USA, and studied for its degradation of phenanthrene as a sole carbon source. The initial 3,4-C dioxygenation was faster than 1,2-C dioxygenation in the first 3-day culture. However, 1-hydroxy-2-naphthoic acid derived from 3,4-C dioxygenation degraded much slower than 2-hydroxy-1-naphthoic acid derived from 1,2-C dioxygenation. Slow degradation of 1-hydroxy-2-naphthoic acid relative to 2-hydroxy-1-naphthoic acid may trigger 1,2-C dioxygenation faster after 3 days of culture. High concentrations of 5,6- and 7,8-benzocoumarins indicated that meta-cleavage was the major degradation mechanism of phenanthrene-1,2- and -3,4-diols. Separate cultures with 2-hydroxy-1-naphthoic acid and 1-hydroxy-2-naphthoic acid showed that the degradation rate of the former to naphthalene-1,2-diol was much faster than that of the latter. The two upper metabolic pathways of phenanthrene are converged into naphthalene-1,2-diol that is further metabolized to 2-carboxycinnamic acid and 2-hydroxybenzalpyruvic acid by ortho- and meta-cleavages, respectively. Transformation of naphthalene-1,2-diol to 2-carboxycinnamic acid by this strain represents the first observation of ortho-cleavage of two rings-PAH-diols by a Gram-negative species.

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Utilization of mixtures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment

Cited by 32 publications

References 27 publications

Characterization of selected actinomycetes degrading polyaromatic hydrocarbons in liquid culture and spiked soil

Characterization of selected actinomycetes degrading polyaromatic hydrocarbons in liquid culture and spiked soil

Degradation of polycyclic aromatic hydrocarbons in soil by a two-step sequential treatment

Degradation of phenanthrene by Burkholderia sp. C3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleavage of naphthalene-1,2-diol

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