Utilization of naproxen by Amycolatopsis sp. Poz 14 and detection of the enzymes involved in the degradation metabolic pathway

Alanís-Sanchez, B. M.; Pérez‐Tapia, Sonia Mayra; Vázquez-Leyva, Said; Mejía‐Calvo, Ignacio; Macías-Palacios, Z.; Vallejo-Castillo, Luis; Flores-Ortíz, César Mateo; Guerrero–Barajas, Claudia; Cruz-Maya, Juan Antonio; Jan‐Roblero, Janet

doi:10.1007/s11274-019-2764-0

Cited by 13 publications

(3 citation statements)

References 36 publications

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“…The reason for this increase is because YE provides a carbon source for bacterial growth, and promotes rapid growth and reproduction of the bacteria, increasing the rate of bacterial growth, as shown in other studies (Alanis‐Sánchez et al . 2019), subsequently, the BbF can be rapidly removed in the presence of a larger number of bacteria. This result highlights that our isolate has a higher degradation efficiency PAH of five‐rings and that it can be considered for the bioremediation of environments with recalcitrant PAHs such as BbF.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

Treviño-Trejo

Alvarez-Hernández

Cruz-Maya

et al. 2021

Lett Appl Microbiol

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Benzo(b)fluoranthene (BbF) is a polycyclic aromatic hydrocarbon (PAH) with five fused benzene rings; it is a highly recalcitrant compound and a priority environmental pollutant due to its detrimental effects on human health and the survival of wild animals. Biodegradation of BbF by microorganisms is an attractive alternative, and few studies have been focused on this issue. In this work, bacteria with the ability to degrade BbF were isolated and selected. The capability of the isolates to tolerate concentrations of 50 and 75 mg l−1 of BbF in liquid medium was evaluated. The selected isolates were identified by the 16S rRNA gene sequencing as belonging to Bacillus, Gordonia, Pseudomonas, Rhodococcus, Ochrobactrum, and Amycolatopsis. All isolates were tolerant and grew at the BbF concentrations tested, some isolates were more competitive than others, and the most prominent was Amycolatopsis sp. Ver12, which removed 47% of BbF, furthermore, with the addition of yeast extract, removed 59% of the compound. In summary, the report shows that Amycolatopsis sp. Ver12 can degrade BbF efficiently and could be considered for bioremediation of BbF‐contaminated environments.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

Treviño-Trejo

Alvarez-Hernández

Cruz-Maya

et al. 2021

Lett Appl Microbiol

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“…Hence, its degradation involves enzymes engaged in the degradation of two fused aromatic rings. Only after the degradation of one of them is the activity of typical dioxygenases cleaving a single aromatic ring possible, which significantly extends the degradation process of naproxen [39,43]. On the other hand, diclofenac has two aromatic rings linked together by an amine bridge.…”

Section: Development Of the Qualitative Composition Of The Bioprepara...mentioning

confidence: 99%

Application of Immobilized Biocatalysts in the Biotransformation of Non-Steroidal Anti-Inflammatory Drugs

2023

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Among the micropollutants identified in the environment, non-steroidal anti-inflammatory drugs (NSAIDs) dominate more and more often. This is due to both the high consumption and low efficiency of biological wastewater treatment plants, where the initial transformation of NSAIDs most often takes place. The solution to the problem may be using preparations supporting activated sludge in sewage treatment plants in the biodegradation of NSAIDs. Therefore, the research aimed to develop a biopreparation stimulating the activated sludge of the sewage treatment plant to decompose paracetamol and selected NSAIDs. This biopreparation is based on strains of Stenotrophomonas maltophilia KB2, Planococcus sp. S5, Bacillus thuringiensis B1(2015b), and Pseudomonas moorei KB4 immobilized on a plant sponge. As a result of the tests, it was shown that the optimal species composition of the proposed preparation includes all tested strains immobilized on a carrier with a mass of 1.2 g/L. The system optimization showed that the optimal amount of strains on the carrier was 17 mg/g of the carrier, 15 mg/g of the carrier, 18 mg/g of the carrier, and 20 mg/g of the carrier for KB4, B1(2015b), KB2, and S5, respectively. The presence of phenol stimulated the degradation of the tested drugs, and this effect deepened with increasing phenol concentration. At the same time, the degradation rate of the mixture of NSAIDs in the presence of phenol did not depend on the amount of biomass. The lack of inhibition in the presence of an additional co-contaminant, i.e., phenol, indicates that the preparation constructed in this way has a chance of being used in sewage treatment plant systems, where introduced strains are exposed to various aromatic compounds.

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“…Receptors transduce the signal to switch the orientation ( Escherichia coli ) [10] or speed (rhizobia) [11] of the flagellum. Four receptors have been best characterized in E. coli, responding to compounds such as sugars ( trg , taxis to ribose and galactose), amino acids ( tar to aspartate; tsr to serine) and dipeptides [10, 12]. An additional sensor responds to oxygen ( aer ).…”

Section: Introductionmentioning

confidence: 99%

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

et al. 2022

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Biotin is a key cofactor of metabolic carboxylases, although many rhizobial strains are biotin auxotrophs. When some of these strains were serially subcultured in minimal medium, they showed diminished growth and increased excretion of metabolites. The addition of biotin, or genetic complementation with biotin synthesis genes resulted in full growth of Rhizobium etli CFN42 and Rhizobium phaseoli CIAT652 strains. Half of rhizobial genomes did not show genes for biotin biosynthesis, but three-quarters had genes for biotin transport. Some strains had genes for an avidin homologue (rhizavidin), a protein with high affinity for biotin but an unknown role in bacteria. A CFN42-derived rhizavidin mutant showed a sharper growth decrease in subcultures, revealing a role in biotin storage. In the search of biotin-independent growth of subcultures, CFN42 and CIAT652 strains with excess aeration showed optimal growth, as they also did, unexpectedly, with the addition of aspartic acid analogues α- and N-methyl aspartate. Aspartate analogues can be sensed by the chemotaxis aspartate receptor Tar. A tar homologue was identified and its mutants showed no growth recovery with aspartate analogues, indicating requirement of the Tar receptor in such a phenotype. Additionally, tar mutants did not recover full growth with excess aeration. A Rubisco-like protein was found to be necessary for growth as the corresponding mutants showed no recovery either with high aeration or aspartate analogues; also, diminished carboxylation was observed. Taken together, our results indicate a route of biotin-independent growth in rhizobial strains that included oxygen, a Tar receptor and a previously uncharacterized Rubisco-like protein.

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Utilization of naproxen by Amycolatopsis sp. Poz 14 and detection of the enzymes involved in the degradation metabolic pathway

Cited by 13 publications

References 36 publications

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

Application of Immobilized Biocatalysts in the Biotransformation of Non-Steroidal Anti-Inflammatory Drugs

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

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