Utilization of UPLC/Q‐TOF‐MS‐Based Metabolomics and AFLP‐Based Marker‐Assisted Selection to Facilitate/Assist Conventional Breeding of Polygala tenuifolia

Abstract

As one of the most important traditional Chinese medicine, the quality of Polygala tenuifolia is difficult to control and a new method must be established to facilitate/assist the breeding of P. tenuifolia. In this study, UPLC/Q-TOF-MS-based metabolomics analysis was performed to determine the chemical composition and screen metabolite biomarkers according to agronomic traits. A total of 29 compounds and 18 metabolite biomarkers were found. AFLP-based marker-assisted selection (MAS) was used to identify molecular marker bands and screen characteristic bands associated with specific agronomic traits. 184 bands and 76 characteristic AFLP bands were found. The correlation network between compounds and characteristic AFLP bands was built, so we may directly breed certain P. tenuifolia herbs with special agronomic traits (or characteristic AFLP bands), which exhibit specific pharmacological functions depending on the content of the active compounds. The proposed method of metabolomics coupled with MAS could facilitate/assist the breeding of P. tenuifolia.

Single-Cell RNA-Sequencing Reveals the Cellular and Genetic Heterogeneity of Skin Scar to Verify the Therapeutic Effects and Mechanism of Action of Dispel-Scar Ointment in Hypertrophic Scar Inhibition

“…In recent years, ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has helped identify various chemical components of traditional Chinese medicine formulae with high sensitivity and resolution. Compounds can be effectively separated using UPLC and then characterised by MS to verify their quality and pharmacological activity for future in vitro and in vivo studies [ 7 – 10 ]. Therefore, we performed a UPLC-Q-TOF-MS-based metabolomics analysis of DSO to determine its chemical composition, which led to the identification and exploration of a total of 74 compounds, including tanshinone II b, hydroxysafflor yellow A, leonurine, stachydrine, quercetin, and senkyunolide.…”