Utilization of sea water based media for the production and characterization of cellulase by Fusarium subglutinans MTCC 11891

Indira, Dash; Balasubramanian, P.; Thirugnanam, A.; Jayabalan, R.

doi:10.1016/j.bcab.2016.06.006

Cited by 14 publications

(3 citation statements)

References 11 publications

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“…Industrial cellulase cocktails, including Accellerase-1500 have been shown to be effective on amorphous and crystalline cellulose but exhibited minor inhibitory effects 81 . In accordance with this, the production of seawater optimised cocktails has been explored using halophilic organisms in seawater-based production media such as Fusarium subglutinans MTCC11891 that produced thermostable, pH stable, and halophilic cellulases with optimal activity observed 0-8-1M NaCl 82 . Halophilic organisms such as Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

Leadbeater,

Bruce

2024

Sci Rep

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Realising a fully circular bioeconomy requires the valorisation of lignocellulosic biomass. Cellulose is the most attractive component of lignocellulose but depolymerisation is inefficient, expensive and resource intensive requiring substantial volumes of potable water. Seawater is an attractive prospective replacement, however seawater tolerant enzymes are required for the development of seawater-based biorefineries. Here, we report a halophilic cellobiohydrolase SMECel6A, identified and isolated from a salt marsh meta-exo-proteome dataset with high sequence divergence to previously characterised cellobiohydrolases. SMECel6A contains a glycoside hydrolase family 6 (GH6) domain and a carbohydrate binding module family 2 (CBM2) domain. Characterisation of recombinant SMECel6A revealed SMECel6A to be active upon crystalline and amorphous cellulose. Mono- and oligosaccharide product profiles revealed cellobiose as the major hydrolysis product confirming SMECel6A as a cellobiohydrolase. We show SMECel6A to be halophilic with optimal activity achieved in 0.5X seawater displaying 80.6 ± 6.93% activity in 1 × seawater. Structural predictions revealed similarity to a characterised halophilic cellobiohydrolase despite sharing only 57% sequence identity. Sequential thermocycling revealed SMECel6A had the ability to partially reversibly denature exclusively in seawater retaining significant activity. Our study confirms that salt marsh ecosystems harbour enzymes with attractive traits with biotechnological potential for implementation in ionic solution based bioprocessing systems.

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Section: Discussionmentioning

confidence: 99%

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

Leadbeater,

Bruce

2024

Sci Rep

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“…Utilization of seawater for biofuel production reduces stress on freshwater resources while enabling the cultivation of biomass, saccharification, and processing of biofuel over a common platform. Fermentation of ethanol in seawater using S. cerevisiae has reported the production of 0.5 g ethanol per gram of glucose Indira et al (2016;. Composition varies based on geographical areas.…”

Section: Sea Water Based Approachmentioning

confidence: 99%

“…Lignocellulosic biorefinery approaches are expected to reduce the problems related to depletion of fossil fuel resources occurring due to large-scale production of chemicals and biofuels in the future. Systems with improved ecological footprints are gaining attention in the production of valueadded chemicals including biofuels from biomass (Indira et al, 2016). It is estimated that 1.9 to 5.9 m3 fresh water is utilized for the production of one m 3 of bioethanol during the largescale operation.…”

Section: Introductionmentioning

confidence: 99%

Seawater – a sustainable solution for the freshwater drain in bioethanol industries

Jayabalan

2019

Maejo Int. J. Energ. Environ. Comm. or MIJEEC

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Consuming fresh water for the production of bioethanol cannot be considered as a sustainable approach due to the worldwide threats on the availability of fresh water in the future. Seawater has been studied as a reaction medium by several research groups in fermentative, enzymatic and chemo-catalytic applications. Several reports concluded that the utilization of seawater would be highly promising and hence the prospects at the interface between biology and chemistry are predictable for holistic innovations and further research. The policies made by several countries have also recommended the implementation of alternative technologies to reduce the consumption of fresh water in industries. This serious situation warrants the development of efficient, ecofriendly, sustainable practices for achieving the sustainability in the global fine and specialty chemicals’ industry.

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Engineering Architectures for Biofuel Cells

Udaya

Devadas

2021

Biofuel Cells

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Utilization of sea water based media for the production and characterization of cellulase by Fusarium subglutinans MTCC 11891

Cited by 14 publications

References 11 publications

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

Seawater – a sustainable solution for the freshwater drain in bioethanol industries

Engineering Architectures for Biofuel Cells

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