Utilization of solid ‘elemental’ sulfur by the phototrophic purple sulfur bacterium Allochromatium vinosum: a sulfur K-edge X-ray absorption spectroscopy study

Franz, Bettina; Lichtenberg, Henning; Hormes, J.; Modrow, Hartwig; Dahl, Christiane; Prange, Alexander

doi:10.1099/mic.0.2006/003954-0

Cited by 74 publications

(66 citation statements)

References 34 publications

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“…We would also like to mention Alvin_0232, a probable outer membrane adhesin. Its abundance is significantly higher on elemental sulfur but not on sulfide and thiosulfate, and we therefore consider it a candidate for providing the necessary contact between the water-insoluble elemental sulfur and the bacterial cells (26). Relative fold changes in mRNA levels above 2 (green) were considered significant enhancement.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast to the oxidation of sulfide and thiosulfate, where sulfur globules appear inside the cells minutes after inoculation, oxidation of elemental sulfur first requires uptake of this virtually water-insoluble substrate. Sulfur globules start to appear about 3 h after inoculation of a culture with elemental sulfur (26). Hence, the incubation period was prolonged accordingly, and cells were harvested after 24 h when elemental sulfur (50 mM) was the electron donor.…”

Section: Methodsmentioning

confidence: 99%

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A Comparative Quantitative Proteomic Study Identifies New Proteins Relevant for Sulfur Oxidation in the Purple Sulfur Bacterium Allochromatium vinosum

Weißgerber

Sylvester

Kröninger

et al. 2014

Appl Environ Microbiol

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bIn the present study, we compared the proteome response of Allochromatium vinosum when growing photoautotrophically in the presence of sulfide, thiosulfate, and elemental sulfur with the proteome response when the organism was growing photoheterotrophically on malate. Applying tandem mass tag analysis as well as two-dimensional (2D) PAGE, we detected 1,955 of the 3,302 predicted proteins by identification of at least two peptides (59.2%) and quantified 1,848 of the identified proteins. Altered relative protein amounts (>1.5-fold) were observed for 385 proteins, corresponding to 20.8% of the quantified A. vinosum proteome. A significant number of the proteins exhibiting strongly enhanced relative protein levels in the presence of reduced sulfur compounds are well documented essential players during oxidative sulfur metabolism, e.g., the dissimilatory sulfite reductase DsrAB. Changes in protein levels generally matched those observed for the respective relative mRNA levels in a previous study and allowed identification of new genes/proteins participating in oxidative sulfur metabolism. One gene cluster (hyd; Alvin_2036-Alvin_2040) and one hypothetical protein (Alvin_2107) exhibiting strong responses on both the transcriptome and proteome levels were chosen for gene inactivation and phenotypic analyses of the respective mutant strains, which verified the importance of the so-called Isp hydrogenase supercomplex for efficient oxidation of sulfide and a crucial role of Alvin_2107 for the oxidation of sulfur stored in sulfur globules to sulfite. In addition, we analyzed the sulfur globule proteome and identified a new sulfur globule protein (SgpD; Alvin_2515).

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Comparative Quantitative Proteomic Study Identifies New Proteins Relevant for Sulfur Oxidation in the Purple Sulfur Bacterium Allochromatium vinosum

Weißgerber

Sylvester

Kröninger

et al. 2014

Appl Environ Microbiol

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“…There is a continuing discussion in the literature concerning the nature of the solid phase sulfur produced and consumed by the wide variety of microbes that interact with elemental sulfur (Lee et al, 2007;Franz et al, 2007;Urich et al, 2006;Prange et al, 2002a;George et al, 2002;Prange et al, 2002b). Given the extent of control these bacteria exert over solid sulfur it could be expected that they would be able to mobilise and oxidise all of the common allotropic forms of sulfur.…”

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The preferential oxidation of orthorhombic sulfur during batch culture

2009

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The formation of sulfur is predicted by the current understanding of the mechanisms involved in mineral sulfide oxidation and observed in studies of the leaching products that accumulate on the surface of the mineral. Sulfur oxidising bacteria can exploit this energy source and can remove a potentially 'rate-limiting' diffusion barrier. In this study on the activity of sulfur oxidising bacteria cultured on mixed solid sulfur allotropes, it was observed that a heterogeneous culture preferentially oxidised the orthorhombic allotrope and no significant growth on the polymeric allotrope could be demonstrated.

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“…3A). XANES spectra were recorded at the DCM beamline at the CAMD, Baton Rouge, LA, and analyzed quantitatively as described previously (8). The errors of the percentages of contribution of sulfur species (Table 1) were estimated to be less than Ϯ10% (absolute value) (21,22).…”

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confidence: 99%

“…In this context, the hypothesis by Urich et al (31) should be noted; based on the crystal structure of the sulfur oxygenase reductase from the thermoacidophilic archaeon "Aquifex aeolicus" and theoretical considerations, only linear sulfur and not cyclic sulfur species can serve as a substrate for this enzyme. Furthermore, Franz et al (8) found evidence that Allochromatium vinosum uses only the sulfur chain fraction of elemental sulfur and is unable to take up cyclo-octasulfur.…”

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In Situ Analysis of Sulfur Species in Sulfur Globules Produced from Thiosulfate by Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes

et al. 2007

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The Firmicutes Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes convert thiosulfate, forming sulfur globules inside and outside cells. X-ray absorption near-edge structure analysis revealed that the sulfur consisted mainly of sulfur chains with organic end groups similar to sulfur formed in purple sulfur bacteria, suggesting the possibility that the process of sulfur globule formation by bacteria is an ancient feature.

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Utilization of solid ‘elemental’ sulfur by the phototrophic purple sulfur bacterium Allochromatium vinosum: a sulfur K-edge X-ray absorption spectroscopy study

Cited by 74 publications

References 34 publications

A Comparative Quantitative Proteomic Study Identifies New Proteins Relevant for Sulfur Oxidation in the Purple Sulfur Bacterium Allochromatium vinosum

A Comparative Quantitative Proteomic Study Identifies New Proteins Relevant for Sulfur Oxidation in the Purple Sulfur Bacterium Allochromatium vinosum

The preferential oxidation of orthorhombic sulfur during batch culture

In Situ Analysis of Sulfur Species in Sulfur Globules Produced from Thiosulfate by Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes

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