Xanthan is microbial polysaccharide with outstanding
rheological properties, non-toxic nature, biodegradability, and
biocompatibility. This biopolymer is widely used in food,
biomedical, pharmaceutical, petrochemical, chemical and textile
industry. Industrial xanthan production is generally conducted by
aerobic submerged cultivation of Xanthomonas campestris strains on
the media with glucose or sucrose under optimal conditions.
Results from previous research indicate that xanthan can be
successfully produced on media containing crude glycerol from
biodiesel industry by different Xanthomonas species. The aim of
this study was to examine the course of xanthan biosynthesis by
the reference strain X. campestris ATCC 13951 in lab-scale
bioreactor on medium containing crude glycerol generated in
domestic biodiesel factory. The bioprocess was monitored by the
analysis of cultivation medium samples taken in predetermined time
intervals, and its success was estimated based on the xanthan
concentration in the medium, separated biopolymer average
molecular weight and degree of nutrients conversion. At the end of
bioprocess, cultivation medium contained 12.34 g/L of xanthan with
the average molecular weight of 3.04∙105 g/mol. Within this study,
the achieved degree of glycerol, total nitrogen and total
phosphorous conversion were 75.91%, 53.27% and 38.96%,
respectively.