Utilization of sugar cane bagasse hemicellulosic hydrolysate by pichia stipitis for the production of ethanol

Roberto, Inês Conceição; Lacis, Lynda S.; Barbosa, M.F.S.; Mancilha, Ismael Maciel de

doi:10.1016/0032-9592(91)80003-8

Cited by 138 publications

(62 citation statements)

References 15 publications

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“…Weak acids (formic, acetic, and levulinic acid), furan derivatives (furfural, 5-hy-droxymethylfurfural) from sugar degradation, phenol compounds from lignin degradation are considered to be potential fermentation inhibitors from pretreated lignocellulose [15]. In order to improve the fermentability of yeast, it can be done from the following two aspects, one is to make the yeast to adapt to the prehydrolyzate step by step, and another is to adopt methods to remove the inhibitors from the prehydrolyzate.…”

Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam stripping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile compounds from the acid hydrolyzate and the filtrate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phenolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fermentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

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Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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“…It has been reported elsewhere in the literature [19] that furfural concentration should be at a level of 1.0 g/L in order to present problems for yeast. The formation of acetic acid was more pronounced in the pretreatment condition with high temperature and addition of oxygen pressure.…”

Section: Effect Of Fermentative Inhibitorsmentioning

confidence: 99%

Production of ethanol from hemicellulose fraction of cocksfoot grass using pichia stipitis

Njoku

Iversen

Uellendahl

et al. 2013

sustain chem process

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Background: In this study, cocksfoot grass (Dactylis glomerata), an abundant lignocellulosic biomass was pretreated using different operational parameters using wet explosion (WEx) pretreatment for accessing the bioethanol potential of the hemicellulose fraction. Utilization of the hemicellulose liquid hydrolysate to ethanol is essential for economically feasible cellulosic ethanol processes. Fermentation of the separated hemicellulose liquid hydrolysates obtained after the WEx pretreatment was done by Pichia stipitis CBS 6054 (Scheffersomyces stipitis).

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“…To maximize the ethanol yield, the production organism should be capable of utilizing all pentoses and hexoses present in the lignocellulose, and, furthermore, it is important that the production organism has a high inhibitor tolerance, since the lignocellulose hydrolysates contain inhibitory substances. Dependent on the nature of the inhibitors, the toxicity may be removed by the use of a variety of detoxification methods, but doing so will greatly increase the cost of operation (11,29,31,114,151,163). Furthermore, for ethanol production, a high product concentration diminishes the cost involved in ethanol recovery, which means that a high ethanol tolerance is preferred.…”

Section: Xylose Utilizationmentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae

Østergaard

Olsson

Nielsen

2000

Microbiol Mol Biol Rev

406

213

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SUMMARY Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over time, and today S. cerevisiae serves as a widley used biotechnological production organism as well as a eukaryotic model system. The high transformation efficiency, in addition to the availability of the complete yeast genome sequence, has facilitated genetic manipulation of this microorganism, and new approaches are constantly being taken to metabolicially engineer this organism in order to suit specific needs. In this paper, strategies and concepts for metabolic engineering are discussed and several examples based upon selected studies involving S. cerevisiae are reviewed. The many different studies of metabolic engineering using this organism illustrate all the categories of this multidisciplinary field: extension of substrate range, improvements of producitivity and yield, elimination of byproduct formation, improvement of process performance, improvements of cellular properties, and extension of product range including heterologous protein production.

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Utilization of sugar cane bagasse hemicellulosic hydrolysate by pichia stipitis for the production of ethanol

Cited by 138 publications

References 15 publications

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Production of ethanol from hemicellulose fraction of cocksfoot grass using pichia stipitis

Metabolic Engineering of Saccharomyces cerevisiae

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