Utilization of Sweet Sorghum Juice for the Production of Astaxanthin as a Biorefinery Co-Product by <i>Phaffia rhodozyma</i>

Stoklosa, Ryan J.; Johnston, David B.; Nghiem, Nhuan P.

doi:10.1021/acssuschemeng.7b03154

Cited by 35 publications

(17 citation statements)

References 61 publications

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“…The samples were separated on a Bio-Rad (Hercules, CA, USA) Aminex HPX-87H column equipped with a cation H + guard column. The operation conditions of the HPLC have been previously reported [24,25]. The astaxanthin was extracted from the cells using acetone according to previous methods [24].…”

Section: Methodsmentioning

confidence: 99%

“…The operation conditions of the HPLC have been previously reported [24,25]. The astaxanthin was extracted from the cells using acetone according to previous methods [24]. A 1 mL aliquot of fermentation broth was mixed with 3 mL of water in a glass test tube.…”

Section: Methodsmentioning

confidence: 99%

“…After four days, the culture obtained a very light pink coloration indicating that astaxanthin production had begun and samples could be inoculated. Samples were supplemented with a minimum of 2.0 g/L yeast extract, 0.4 g/L urea, and several minerals according to prior research [24]. The pH of each sample was adjusted to 5.5 followed by filter sterilization through a 0.2-micron filter cup.…”

Section: Astaxanthin Fermentationmentioning

confidence: 99%

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Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

2019

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Developing integrated biorefineries requires the generation of high-value co-products produced alongside cellulosic ethanol. Most industrial yeast strains produce ethanol at high titers, but the small profit margins for generating ethanol require that additional high-value chemicals be generated to improve revenue. The aim of this research was to boost xylose utilization and conversion to high-value co-products that can be generated in an integrated biorefinery. Pretreated sweet sorghum bagasse (SSB) was hydrolyzed in sweet sorghum juice (SSJ) followed by ethanol fermentation. Ethanol was removed from the fermentation broth by evaporation to generate a stillage media enriched in xylose. Candida mogii NRRL Y-17032 could easily grow in non-detoxified stillage media, but a high xylitol yield of 0.55 g xylitol/g xylose consumed was achieved after recovered cells were resuspended in synthetic media containing supplemented xylose. Phaffia rhodozyma ATCC 74219 could be cultivated in non-detoxified stillage media, but astaxanthin generation was increased 4-fold (from 17.5 to 71.7 mg/L) in detoxified media. Future processing strategies to boost product output should focus on a two-step process where the stillage media is used as the growth stage, and a synthetic media for the production stage utilizing xylose generated from SSB through selective hemicellulase enzymes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Astaxanthin Fermentationmentioning

confidence: 99%

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Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

2019

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“…The C/N ratio has a significant influence on cell growth and carotenoid biosynthesis. In other studies, was related that a high C/N ratio increased the carotenoid production yield due to the nitrogen limitation [22,23].…”

Section: Discussionmentioning

confidence: 90%

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

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This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

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“…As a C4 crop, sweet sorghum contains rich fermentable sugars in its stems (such as sucrose, glucose and fructose) and a variety of cultivars and growing characteristics such as high biomass yield and rapid growth, which make it an attractive feedstock for value-added biochemical production. A number of fuels and bio-chemicals have been successfully produced using sweet sorghum juice (SSJ) as feedstocks [7][8][9][10][11][12]. In a previous study, we have shown that SSJ was the preferred feedstock for enduracidin production with approximately equivalent e ciency to that of glucose, and S. fungicidicus M30 can also produce 1.01 ± 0.05 g/L of enduracidin via clari cated SSJ and fed-batch strategy [13].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Enduracidin Accumulation from Sweet Sorghum Juice via Alleviation of Oxidative Damage in Streptomyces Fungicidicus M30

Lü

Chen

et al. 2020

Preprint

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Abstract Background: Sweet sorghum juice is the preferred feedstock for enduracidin production, but the fermentation efficiency still need to be further enhanced. Oxygen-mediated microbial cell damage is an effective way to boost cell growth and metabolite accumulation in aerobic fermentation. However, this strategy on enduracidin production from sweet sorghum juice has not been reported yet. Results: In this study, enduracidin production titer was significantly enhanced when 90 mM Vitamin C was added at 4 d of fermentation. The enhanced T-AOC and antioxidant enzyme activities may help to further understand the mechanism of Vitamin C effect on regulation of enduracidin accumulation in S. fungicidicus M30. The effects of chemical preservative methyl paraben on cell growth and enduracidin production were also evaluated, and 0.04 g/L of methyl paraben had no significant inhibitory effect on cell growth and enduracidin production, indicating that the concentrated sweet sorghum juice assisted by methyl paraben for a long-term storage can be used as the substrate for enduracidin production effectively. Finally, based on the group with 0.04 g/L of methyl paraben and 90 mM Vitamin C, a final enduracidin concentration of 1.14 g/L was achieved from clarificated sweet sorghum juice by M30 strain. Conclusions: This work provided a promising strategy to enhance enduracidin production using sweet sorghum juice via adding the antioxidant to boost antioxidant capacity in S. fungicidicus.

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Utilization of Sweet Sorghum Juice for the Production of Astaxanthin as a Biorefinery Co-Product by Phaffia rhodozyma

Cited by 35 publications

References 61 publications

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Enhancement of Enduracidin Accumulation from Sweet Sorghum Juice via Alleviation of Oxidative Damage in Streptomyces Fungicidicus M30

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