Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Abstract

Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances

“…Sweet sorghum stalk juice containing fermentable sugars and other nutrients has been utilized for BDO production by S. marcescens (Yuan et al, 2017). Under optimized conditions, 109.4 g L -1 of BDO was produced with a productivity of 1.4 g L -1 h -1 in fed-batch fermentation.…”

Biotechnological Approaches to Generate Biogenic Solvents and Energy Carriers from Renewable Resources

“…In addition, sweet sorghum juice, an inexpensive, sugar-rich raw material, was applied for 2,3-BD biosynthesis by Yuan et al, who used a Serratia marcescens strain, another facultative anaerobe. In fed-batch studies carried out in 5 L bioreactors, about 110 g/L 2,3-BD were obtained at a volumetric productivity of 1.40 g/(L•h) and a conversion yield of 0.83 g/g [55].…”

Carrot Discard as a Promising Feedstock to Produce 2,3-Butanediol by Fermentation with P. polymyxa DSM 365

“…These microorganisms are able to use a wide range of six- and five-carbon sugars as carbon sources, such as glucose, fructose, xylose, ribose, and arabinose, among others [ 10 ]. BDO production from different lignocellulosic residues, such as corn stover [ 11 ], sweet sorghum stalk [ 12 ], sugar beet pulp [ 13 ], soybean hull [ 14 ], sugarcane bagasse [ 15 ], oil palm frond [ 16 ], apple pomace [ 17 ], rice straw [ 18 ], and fruit and vegetable residues [ 19 ] has been reported in the literature. Most of these residues present complex structures, cellulose, and hemicellulose, which require a pre-treatment step in order to obtain the simple sugars [ 15 ].…”