Utilization of the By-Product of Corn: Guided Identification of Bioactive Terpenoids from Stigma Maydis (Corn Silk)

Abstract

Stigma maydis (corn silk) (S. maydis) is a food-based by-product of maize and possesses great nutritional and pharmaceutical value. This study aimed to explore bioactive components from S. maydis. By the guidance of bioactivity-guided approach and Global Natural Products Social (GNPS) molecular networking, 12 terpenoids were discovered from S. maydis. The structures of 11 undescribed compounds (1–11) were determined by detailed spectroscopic analyses, single-crystal X-ray diffraction analysis, specific rotation calculations, electronic circular dichroism (ECD) calculations, and NMR calculations. The neuroprotective and acetylcholinesterase (AChE) inhibitory effects of 1–12 were examined, and most of them showed significant or moderate activities. The underlying neuroprotective mechanism of 4 and 5 was revealed by Hoechst 33258, AO-EB, and JC-1 staining assays. This work illustrated the potential of S. maydis as a prospective natural source of bioactive compounds in food and pharmaceutical industries.

The application and perspective of NMR and MS based strategies for functional compounds mining in medicinal and dietary plants

“…In the classical MN, 223 nodes were annotated by MS 2 spectral library matching, and 96 of which were annotated as terpenoids. By combination of bioactivity-guided approaches, 12 terpenoids with neuroprotective activities including 11 undescribed terpenoids were discovered from corn silk [104] . The dereplication strategy of methanolic extract from six edible mushroom species of Pleurotus have also achieved [105] , and the bioactive fatty acids and steroids were annotated and identified with nutraceutical properties by using MN-based GNPS.…”

Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

“…Corn silk is a common TCM that people usually take after boiling to achieve the effect of hypoglycemic. Corn silk is rich in flavonoids [ 12 ], polysaccharides [ 13 ], terpenoids [ 14 ] and so on. Corn silk extract has been reported to have hypoglycemia [ 15 ], antioxidation [ 16 ], antitumor [ 17 ], antibacterial [ 18 ] and other pharmacological activities.…”

Optimizing extraction conditions and isolation of bound phenolic compounds from corn silk (Stigma maydis) and their antioxidant effects

“…Silk obtained from maize is regarded as a waste byproduct and typically dumped as waste due to ignorance about its possible benefits. Corn silk contains several bioactive phytochemical compounds, including flavonoids (D. Zhang et al., 2020; H. Zhang et al., 2017), flavone glycosides (Tian et al., 2021), terpenoids (Zhou et al., 2023), and sterols (H. Zhang et al., 2017). It has been determined that corn silk is nontoxic to humans and can be used as the primary element in health‐promoting medications (Saheed et al., 2015; C. Wang et al., 2011).…”

“…Corn silk contains several bioactive phytochemical compounds, including flavonoids (D. H. Zhang et al, 2017), flavone glycosides (Tian et al, 2021), terpenoids (Zhou et al, 2023), and sterols (H. Zhang et al, 2017). It has been determined that corn silk is nontoxic to humans and can be used as the primary element in health-promoting medications (Saheed et al, 2015;C.…”