Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides

Villaluenga, Cristina Martínez; Wardeńska, Monika; Pilarski, Radosław; Bednarczyk, Marek; Gulewicz, Krzysztof

doi:10.3409/1734916044527502

Cited by 59 publications

(58 citation statements)

References 23 publications

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“…Casas‐Perez and Edens found that the administration of probiotics (lactic acid bacteria) via the in ovo technique, should be done on the 18th day of incubation. In ovo injection of some oligosaccharides on the 12th day of incubation is a preferable time that could improve growth performance, nutrient digestibility and feed efficiency, development of the immune system, and meat quality . The in ovo chicken transfection technique using ethylenediaminetetraacetic acid complemented with lipoplexes has also been evaluated to develop a puncture‐free method of producing transgenic chicks .…”

Section: Basics Of In Ovo Technologymentioning

confidence: 99%

In ovodelivery of various biological supplements, vaccines and drugs in poultry: current knowledge

et al. 2019

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The technique of delivering various nutrients, supplements, immunostimulants, vaccines, and drugs via the in ovo route is gaining wide attention among researchers worldwide for boosting production performance, immunity and safeguarding the health of poultry. It involves direct administration of the nutrients and biologics into poultry eggs during the incubation period and before the chicks hatch out. In ovo delivery of nutrients has been found to be more effective than post‐hatch administration in poultry production. The supplementation of feed additives, nutrients, hormones, probiotics, prebiotics, or their combination via in ovo techniques has shown diverse advantages for poultry products, such as improved growth performance and feed conversion efficiency, optimum development of the gastrointestinal tract, enhancing carcass yield, decreased embryo mortality, and enhanced immunity of poultry. In ovo delivery of vaccination has yielded a better response against various poultry pathogens than vaccination after hatch. So, this review has aimed to provide an insight on in ovo technology and its potential applications in poultry production to deliver different nutrients, supplements, beneficial microbes, vaccines, and drugs directly into the developing embryo to achieve an improvement in post‐hatch growth, immunity, and health of poultry. © 2019 Society of Chemical Industry

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Section: Basics Of In Ovo Technologymentioning

confidence: 99%

In ovodelivery of various biological supplements, vaccines and drugs in poultry: current knowledge

et al. 2019

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“…It has been noted that the colonization of GIT with nonpathogenic microorganisms creates a protective surface against pathogens (Mahroop Ra, Raja, & Mohamed Im, ). In ovo administration of probiotic bacteria may lead to GIT colonization before the first contact with environmental microorganisms (Villaluenga, Wardeńska, Pilarski, Bednarczyk, & Gulewicz, ). The initial microbe inoculation may protect the gut of the chick from pathogenic bacteria by competitive exclusion mechanisms, which are based on bacterial interactions mediated by competition for mucosal adhesion sites and nutrients (Majidzadeh Heravi et al., ).…”

Section: Introductionmentioning

confidence: 99%

Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery

Aleksandrzak‐Piekarczyk

Puzia

Żylińska

et al. 2018

MicrobiologyOpen

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The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk and hen feces) with respect to their applicative potential to colonize gastrointestinal track of chickens before hatching from an egg. In vitro phenotypic characterization of lactobacilli strains included the investigation of the important prerequisites for persistence in gastrointestinal tract, such as a capability to survive in the presence of bile salts and at low pH, enzymatic and sugar metabolic profiles, adhesion abilities, and resistance to osmolytes, temperature, and antibiotics. Regarding the resistance of lactobacilli to most of the various stress factors tested, the milk isolate Lactobacillus plantarum IBB3036 showed better abilities than the chicken feces isolate Lactobacillus salivarius IBB3154. However, regarding the acidification tolerance and adherence ability, L. salivarius IBB3154 revealed better characteristics. Use of these two selected lactobacilli isolates together with proper prebiotics resulted in the preparation of two S1 and S2 bioformulations, which were injected in ovo into hen Cobb500 FF fertilized eggs. Furthermore, in vivo tests assessing the persistence of L. plantarum IBB3036 and L. salivarius IBB3154 in the chicken gastrointestinal tract was monitored by PCR-based classical and quantitative techniques and revealed the presence of both strains in fecal samples collected 3 days after hatching. Subsequently, the number of L. salivarius IBB3154 increased significantly in the chicken intestine, whereas the presence of L. plantarum IBB3036 was gradually decreased.

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“…Substances administered in ovo during the embryonic development of birds reach the intestines and affect the development of the gastrointestinal tract before hatching. Villaluenga et al [16] demonstrated that the optimal time for the injection of a prebiotic is the 12 th day of embryonic development. In comparison with injections on d 1, 8 and 17, a significantly higher number of bifidobacteria was observed in the gut.…”

Section: Introductionmentioning

confidence: 99%

The influence of in ovo injection with the prebiotic DiNovo® on the development of histomorphological parameters of the duodenum, body mass and productivity in large-scale poultry production conditions

Sobolewska

Elminowska-Wenda

Bogucka

et al. 2017

J Animal Sci Biotechnol

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BackgroundAmong various feed additives currently used in poultry nutrition, an important role is played by bioactive substances, including prebiotics. The beneficial effect of these bioactive substances on the gastrointestinal tract and immune system give rise to improvements in broiler health and performance nutrition, thus increasing the productivity of these birds. An innovative method for introducing bioactive substances into chickens is the in ovo injection into eggs intended for hatching. The aim of the study was to evaluate the development of histomorphological parameters of the duodenum and productivity in chickens injected in ovo with the prebiotic DiNovo® (extract of Laminaria species of seaweed, BioAtlantis Ltd., Ireland) on d 12 of incubation, under large - scale, high density poultry production conditions.ResultsThere was no significant impact of the injection of DiNovo® prebiotic on the production parameters of broiler chickens (body weight, FCR, EBI and mortality) obtained on d 42 of rearing. No significant impact of the DiNovo® injection on the duodenum weight and length was observed, as well as on the CSA, diameter and muscular layer thickness of the duodenum. The in ovo injection of DiNovo® significantly increased the width of the duodenal villi (P < 0.05) and crypt depth (P < 0.01) of chickens on d 21 of rearing. Other histomorphological parameters of duodenal villi at d 42 of chickens rearing such as: the height, width, and cross section area of villi were significantly greater in chickens from the control group compared to those from the DiNovo® group (P < 0.05 and P < 0.01).ConclusionsIn conclusion, this study demonstrates that injection of DiNovo® prebiotic into the air chamber of egg significantly influences the histomorphological parameters on d 21 of rearing without negatively affecting productivity in chickens at the end of rearing.

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Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides

Cited by 59 publications

References 23 publications

In ovodelivery of various biological supplements, vaccines and drugs in poultry: current knowledge

In ovodelivery of various biological supplements, vaccines and drugs in poultry: current knowledge

Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery

The influence of in ovo injection with the prebiotic DiNovo® on the development of histomorphological parameters of the duodenum, body mass and productivity in large-scale poultry production conditions

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