Utilization of Waste Syrup for Production of Polyunsaturated Fatty Acids and Xanthophylls by Aurantiochytrium

Iwasaka, Hiroaki; Aki, Tsunehiro; Adachi, Hirofumi; Watanabe, Kenshi; Kawamoto, Seiji; Ono, Kazuhisa

doi:10.5650/jos.62.729

Cited by 29 publications

(7 citation statements)

References 23 publications

(20 reference statements)

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“…Thraustochytrids are heterotrophic marine stramenopilan protists. − They have recently attracted great attention due to their potential for the production of long-chain omega-3 oils − and astaxanthin − that are important for human health and aquaculture. Numerous studies have focused on the optimization of culture conditions (e.g., salinity, pH, temperature, and media) for higher maximal biomass and value-added products with significant success. − …”

Section: Introductionmentioning

confidence: 99%

Enhanced Production of Fatty Acids and Astaxanthin inAurantiochytriumsp. by the Expression ofVitreoscillaHemoglobin

Suen

Hong-mei

Huang

et al. 2014

J. Agric. Food Chem.

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Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.

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Section: Introductionmentioning

confidence: 99%

Enhanced Production of Fatty Acids and Astaxanthin inAurantiochytriumsp. by the Expression ofVitreoscillaHemoglobin

Suen

Hong-mei

Huang

et al. 2014

J. Agric. Food Chem.

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“…T66 [31] and that xanthophylls can be produced by Aurantiochytrium sp. KH105 [32]. In order to nd the unknown additional value of the biomass mixture, further studies should analyze organic compounds adding to DHA and EPA produced by the strain L3W growing on the Japanese mustard spinach.…”

Section: Production Of Dha and Epa From Pasteurized Solid Food Wastementioning

confidence: 99%

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Suenaga

Nakai

Umehara

et al. 2023

Waste Biomass Valor

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Purpose: This study aimed at valorizing solid food waste containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).Methods: Aurantiochytrium sp. L3W that produces DHA and EPA was cultivated on eight types of solid food waste: sake lees (SL), crown daisy, Japanese mustard spinach (JMS), soy sauce residue, lemon peel (LP), orange peel, grape skin, and Hiroshimana old pickle (HOP). The biomass mixture of the remaining food waste and strain L3W was analyzed for DHA and EPA. To characterize the types of food waste, the leachability of dissolved organic carbon (DOC) and dissolved nitrogen (DN) were compared.Results: The strain L3W grew on both pasteurized and unsterilized food waste such as SL and JMS. Elution of DOC and DN from the food waste might be a factor affecting the growth of strain L3W.However, the strain L3W might utilize solid-state organic compounds in JMS. Despite the unsterile conditions, the biomass mixture of SL contained both DHA and EPA, whereas DHA was found in the biomass mixtures of JMS, LP and HOP, thereby con rming the valorization of these types of solid food waste. Unsterile mass cultivation of the strain L3W using SL and HOP in a 200 L tank also produced a biomass mixture containing 12.6 mg-DHA/g and 0.217 mg-EPA/g. These DHA and EPA contents were 1500-times and 37-times higher, respectively, than that in commercial poultry feed, indicating that these mixtures could be used as an additive in poultry feed. Statement Of NoveltyThe demand for docosahexaenoic and eicosapentaenoic acids (DHA and EPA) from sh oil has been increasing because of its bene cial use in the aquaculture and food industries but sh oil resources are now becoming unsustainable. Therefore, we have focused on using the heterotrophic microorganism, Aurantiochytrium sp. L3W, to produce DHA and EPA. The present study demonstrated that solid food waste can be used for culturing Aurantiochytrium sp. L3W without sterilization and hydrolysis pretreatments and provided evidence that the strain L3W may utilize solid-state carbon and nitrogen. We obtained biomass mixtures consisting of the remaining food waste and analyzed them for DHA and EPA. The DHA and EPA contents were 1500-times and 37-times higher, respectively, than those in commercial poultry feed. This is the rst report on valorizing solid food waste as a source of PUFAs that are potentially usable as an additive for poultry feed to produce products enriched with PUFAs, such as eggs.

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“…Among the various microorganisms considered as biocatalysts for biorefineries, thraustochytrids 2) are expected to be key players because they produce various lipids, such as polyunsaturated fatty acids 3) , squalene 4) , and carotenoids 5,6) by assimilation of organic compounds as well as sugars. Recent research has reported a lipid fermentation system by thraustochytrids using unutilized biomass, including food waste 7,8) , macroalgae 9) , or waste glycerol 10) . In this study, we attempted to utilize sugarcane bagasse as an abundant terrestrial lignocellulosic biomass 11) as a substrate for lipid production by the thraustochytrid, Aurantiochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Sugarcane Bagasse as a Substrate for Lipid Production by <i>Aurantiochytrium</i> sp.

et al. 2022

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Materials and Methods Microorganisms, culture media, and reagentsA. limacinum SR21 (ATCC MYA-1381) was cultured in GPY medium containing 30 g/L glucose, 6 g/L Hipolypepton (Nihon Pharmaceutical, Tokyo, Japan) , 2 g/L yeast extract, and 20 g/L artificial sea salt (Sigma-Aldrich, St. Louis, MO, USA) at pH 6.5, 28℃ and 180 rpm for 48 h. Reagents were purchased from Nacalai Tesque (Osaka, Japan) , Sigma-Aldrich, and Tokyo Kasei (Tokyo, Japan) unless otherwise indicated.

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Utilization of Waste Syrup for Production of Polyunsaturated Fatty Acids and Xanthophylls by Aurantiochytrium

Cited by 29 publications

References 23 publications

Enhanced Production of Fatty Acids and Astaxanthin inAurantiochytriumsp. by the Expression ofVitreoscillaHemoglobin

Enhanced Production of Fatty Acids and Astaxanthin inAurantiochytriumsp. by the Expression ofVitreoscillaHemoglobin

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Utilization of Sugarcane Bagasse as a Substrate for Lipid Production by <i>Aurantiochytrium</i> sp.

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