Utilizing a Metal Inlet Coiled with Copper Wire as the Ion Source for Ambient Ionization Mass Spectrometry

Abstract

In ambient ionization mass spectrometry (MS), a customized metal inlet is typically adapted to the orifice of the mass spectrometer for ease of introduction of the sample. We herein explore that the metal inlet coiled with a copper wire (∼50 μm) can be directly used as an ion source to induce corona discharge-like processes for ionization of analytes in the gas phase. When the metal inlet is subjected to a high voltage in the mass spectrometer, the electric field provided by the mass spectrometer enables the generation of corona discharge to ionize volatile/semivolatile analytes derived from the sample in the condensed phase. The limit of detection for azulene derived from the aqueous sample was as low as ∼1 pM. Moreover, we also demonstrated the feasibility of coupling ultraviolet−visible absorption spectroscopy with MS by using the metal inlet coiled with a thin copper wire as the interface. Integration of these two techniques enables the simultaneous acquisition of spectra from both instruments for quantitative and qualitative analysis of the sample. Furthermore, we showed that polar and nonpolar analytes in a mixture can be acquired in the same mass spectrum by simply depositing a sample droplet (∼20 μL) on a dielectric substrate near the copper wire-coiled metal inlet of the mass spectrometer. The ionization processes involved both electrospray ionization and corona discharge. To demonstrate the applicability of our method for detecting nonpolar and polar analytes in complex samples, we spiked a nonpolar analyte, benzo[a]pyrene, to a spice sample and successfully detected analytes with different polarities using our approach.