Utilizing adsorbed proteoliposomes trapped in a non-ruptured state on SiO2 for amplified detection of membrane proteins

Granéli, Annette; Rydström, Jan; Kasemo, Bengt Herbert; Höök, Fredrik

doi:10.1016/j.bios.2004.02.013

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…21,29,35 As vesicles were introduced to the sensor, there was a rapid decrease in the frequency and a simultaneous rapid rise in dissipation. After the vesicles reached a critical coverage, they expelled entrapped water as the vesicle fused and ruptured to form a rigid SPB (loss of water mass to bulk media was indicated by an increase in the frequency; see Figure 3), which was indicated by a decrease in dissipation to <0.2 × 10 -6 (as was typically found for SPB formation on silica 18,19,29,32,34,38 ). After exposure to and subsequent removal of bulk BMIM-Cl, the SPB exhibited no change in the adsorbed mass/frequency of the supported bilayer, indicating that no detectable amount of BMIM + adsorbed to the SPB.…”

Section: Experimental Methodsmentioning

confidence: 96%

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

Evans

2008

J. Phys. Chem. B

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Quartz crystal microbalance with dissipation measurements were conducted for a 98/2 mole ratio of 1,2-dielaidoylphosphocholine (DEPC) and 1,2-dimyristoylphosphoglycerol (DMPG) on silica, gold, and a self-assembled monolayer of 11-mercapto-1-undecanol (11MU) and 11-mercaptoundecanoic acid (11MUA) at 50 mol % each. This study demonstrates that vesicles composed of DEPC and DMPG at 98 and 2 mol %, respectively, formed a supported bilayer with unruptured vesicles present when adsorbed onto the self-assembled monolayer. Also, the partially formed supported bilayer apparently deadsorbed in the presence of 1-butyl-3-methylimidazolium chloride, suggesting that surface-bilayer interactions are weaker on a hydrophilic modified gold surface composed of 50/50 11MU/11MUA than the surface-bilayer interactions on silica.

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Section: Experimental Methodsmentioning

confidence: 96%

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

Evans

2008

J. Phys. Chem. B

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“…Gold substrate was used as the IR-reflecting material in this study. There are several reports on the deposition of thin silica films on gold. ,,− The main drawback of these methods is the instability of the silica film if directly deposited on gold. We have recently found that thin layers of SiO 2 can be deposited on gold films with no intervening adhesion layer using plasma enhanced chemical vapor deposition (PECVD) technique. − The technique is based on the decomposition of a mixture of silane gas (SiH 4 ) and nitrous oxide (N 2 O) near the substrate surface, enhanced by the use of a vapor containing electrically charged particles or plasma at 300 °C.…”

Section: Introductionmentioning

confidence: 99%

PM IRRAS Investigation of Thin Silica Films Deposited on Gold. Part 1. Theory and Proof of Concept

et al. 2007

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Polarization modulation infrared reflection absorption spectroscopy (PM IRRAS) was successfully used for the first time to characterize an optically transparent thin oxide film. SiO2 layers of 7 nm thickness were synthesized by plasma enhanced chemical vapor deposition (PECVD) on 200 nm thick gold covered glass slides. Despite the fact that silica is transparent and absorptive to IR radiation when deposited in the form of thin films on a gold surface, it preserves the high metallic reflectivity for the IR light. At grazing angles of incidence of the IR beam, the enhancement of the normal component of the electric field at the interface is comparable to that of Au alone. In addition, the analysis of the structure of a 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid monolayer deposited using the Langmuir-Blodgett technique is demonstrated.

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“…However, the authors did not comment on the type of bonding between the gold surface and the silane multilayer. The group of Granéli and Kasemo reported that by depositing 1 nm of titanium between the gold and the silicon dioxide layer (30 nm in the case of a SPR chip) stable films could be formed, and the formation of supported phospholipid layers was investigated using a quartz crystal microbalance and SPR. − In this case, a 1 nm adhesion layer of titanium was deposited on the top of the gold surface and the silicon dioxide film was deposited using electron-beam evaporation.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Thin Films of SiO_x on Gold Substrates for Surface Plasmon Resonance Studies

Szunerits

Boukherroub

2006

Langmuir

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We report here on the fabrication and characterization of stable thin films of amorphous silica (SiO(x)) deposited on glass slides coated with a 5 nm adhesion layer of titanium and 50 nm of gold, using the plasma-enhanced chemical vapor deposition (PECVD) technique. The resulting surfaces were characterized using atomic force microscopy (AFM), ellipsometry, contact angle measurements, and surface plasmon resonance (SPR). AFM analysis indicates that homogeneous films of silica with low roughness were formed on the gold surface. The deposited silica films showed excellent stability in different solvents and in piranha solution. There was no significant variation in the thickness or in the SPR signal after these harsh treatments. The Au/SiO(x) interfaces were investigated for their potential applications as new surface plasmon resonance sensor chips. Silica films with thicknesses up to 40 nm allowed visualization of the surface plasmon effect, while thicker films resulted in the loss of the SPR characteristics. SPR allowed further the determination of the silica thickness and was compared to ellipsometric results. Chemical treatment of the SiO(x) film with piranha solution led to the generation of silanol surface groups that have been coupled with a trichlorosilane.

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Utilizing adsorbed proteoliposomes trapped in a non-ruptured state on SiO2 for amplified detection of membrane proteins

Cited by 13 publications

References 37 publications

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

PM IRRAS Investigation of Thin Silica Films Deposited on Gold. Part 1. Theory and Proof of Concept

Preparation and Characterization of Thin Films of SiO_x on Gold Substrates for Surface Plasmon Resonance Studies

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Utilizing adsorbed proteoliposomes trapped in a non-ruptured state on SiO2 for amplified detection of membrane proteins

Cited by 13 publications

References 37 publications

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

Supported Phospholipid Membrane Interactions with 1-Butyl-3-methylimidazolium Chloride

PM IRRAS Investigation of Thin Silica Films Deposited on Gold. Part 1. Theory and Proof of Concept

Preparation and Characterization of Thin Films of SiOx on Gold Substrates for Surface Plasmon Resonance Studies

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Preparation and Characterization of Thin Films of SiO_x on Gold Substrates for Surface Plasmon Resonance Studies