“…Due to the importance of cell‐cycle regulation for both the self‐renewal and differentiation of hPSCs, a stable and robust cell‐cycle visualization system could effectively provide more valuable information to broaden the applications of hPSCs. Although the FUCCI system has been widely used in many dynamic models, including zebrafish (Choi et al, 2013), mouse (Abe et al, 2013), and stem cells (Calder et al, 2013; Coronado et al, 2013; Jovic et al, 2013; Roccio et al, 2013; Singh et al, 2013; Singh et al, 2016), a lineage‐specific hPSC‐FUCCI reporter has not yet been generated for versatile and high‐throughput analysis of cell‐cycle behavior during hPSC self‐renewal and differentiation. Here, we have established and validated a robust and universally applicable FUCCI system in hPSCs for continuous and lineage‐specific reporting of cell‐cycle behavior.…”