Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Abstract

In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 μM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 μg of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 ± 0.8 μM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

“…The role of MTs and zinc in cancer development is closely linked and the structure and function of MTs depend strongly on the redox state of zinc and its interactions with proteins (Krizkova et al 2012). A similar increase in the concentration of MTs has previously been documented in skin melanoma cells both in vivo and in vitro (Krizkova et al 2008). Likewise, both oxidative stress associated indices, i.e.…”

“…Interestingly, apart from metal ion homeostasis, detoxification, protection against oxidative damage, and cell proliferation and apoptosis, metallothionein isoforms have been shown to be involved in several aspects of the carcinogenic process, cancer development and progression (Cherian et al 2003;Theocharis et al 2003;Krizkova et al 2008). Elevated MT concentrations in cancer cells are probably related to their increased proliferation and protection against apoptosis (Krizkova et al 2009).…”

Evaluation ofalpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers

“…Moreover, this marker has been found elevated even in other types of cancers such as breast carcinoma [66], head and neck cancer [67], medulloblastoma [35], melanoma [68,69] and other [70][71][72][73][74]. Nevertheless, combination of MT and PSA levels could be of prognostic significance due to possible revealing of false positive results, but this assumption needs to be investigated in greater details.…”

Using of chicken antibodies for metallothionein detection in human blood serum and cadmium‐treated tumour cell lines after dot‐ and electroblotting

“…Analysis of MT by immunochemical methods is a promising technique to obtain more information about MT level and its connection to disease progression. Papers reporting on association of MT level and tumour disease were published recently [58,60,88]. It is common knowledge that MT is able to bind heavy metals and therefore to decrease the therapeutic concentrations of heavy metal-based cytostatics (e.g.…”