Utilizing R Software Package for Dose-Response Studies: The Concept and Data Analysis

Knežević, Stevan Z.; Streibig, J. C.; Ritz, Christian

doi:10.1614/wt-06-161.1

Cited by 434 publications

(410 citation statements)

References 27 publications

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“…, where e is the ED50, the upper limit is d and b denotes the relative slope around e. The above model is a very useful and widely accepted method for addressing the response of plants to increasing doses of any type of xenobiotic [17,18].…”

Section: Resultsmentioning

confidence: 99%

Composition and herbicidal effect of Heracleum sosnowskyi essential oil

Synowiec

Kalemba

2015

Open Life Sciences

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Plants of Heracleum sosnowskyi Manden. are rich in essential oil which might be of herbicidal potential. In this study, we examined 1) the content and chemical composition of H. sosnowskyi essential oil (EO) distilled from seeds and 2) the herbicidal effect of EO in a bioassay against germination of maize and five weed species. As a result, a 5.1% EO yield was obtained from the seeds of H. sosnowskyi. We identified sixty-two compounds of EO that constituted 96% of the total oil. Aliphatic esters were the main constituents of the EO, followed by aliphatic alcohols. The tested seeds showed different susceptibilities to the tested concentrations of EO (0.2 – 7.2 g L

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Section: Resultsmentioning

confidence: 99%

Composition and herbicidal effect of Heracleum sosnowskyi essential oil

Synowiec

Kalemba

2015

Open Life Sciences

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“…Phosphate release above background level was measured for 10 min, and a slope was calculated to determine micromoles of phosphate released per microgram TSP per minute. Dose-response analysis in R was used to calculate the IC 50 , the glyphosate concentration that inhibited EPSPS activity by 50%, and to statistically compare IC 50 values (44).…”

Section: Methodsmentioning

confidence: 99%

Gene amplification confers glyphosate resistance in Amaranthus palmeri

Gaines

Zhang

Wang

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

601

868

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The herbicide glyphosate became widely used in the United States and other parts of the world after the commercialization of glyphosate-resistant crops. These crops have constitutive overexpression of a glyphosate-insensitive form of the herbicide target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Increased use of glyphosate over multiple years imposes selective genetic pressure on weed populations. We investigated recently discovered glyphosate-resistant Amaranthus palmeri populations from Georgia, in comparison with normally sensitive populations. EPSPS enzyme activity from resistant and susceptible plants was equally inhibited by glyphosate, which led us to use quantitative PCR to measure relative copy numbers of the EPSPS gene. Genomes of resistant plants contained from 5-fold to more than 160-fold more copies of the EPSPS gene than did genomes of susceptible plants. Quantitative RT-PCR on cDNA revealed that EPSPS expression was positively correlated with genomic EPSPS relative copy number. Immunoblot analyses showed that increased EPSPS protein level also correlated with EPSPS genomic copy number. EPSPS gene amplification was heritable, correlated with resistance in pseudo-F 2 populations, and is proposed to be the molecular basis of glyphosate resistance. FISH revealed that EPSPS genes were present on every chromosome and, therefore, gene amplification was likely not caused by unequal chromosome crossing over. This occurrence of gene amplification as an herbicide resistance mechanism in a naturally occurring weed population is particularly significant because it could threaten the sustainable use of glyphosate-resistant crop technology.5-enolpyruvylshikimate-3-phosphate synthase | herbicide resistance | mobile genetic element | evolution | Palmer amaranth

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“…Length data were standardized to the percentage of the untreated control in each experiment. Percentage data were analyzed in R software using the drc package to determine and compare GR 50 values (Knezevic et al, 2007).…”

Section: Indaziflam Dose Response and Cross Resistancementioning

confidence: 99%

Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Brabham¹,

Lei

et al. 2014

Plant Physiology

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Cellulose biosynthesis is a common feature of land plants. Therefore, cellulose biosynthesis inhibitors (CBIs) have a potentially broad-acting herbicidal mode of action and are also useful tools in decoding fundamental aspects of cellulose biosynthesis. Here, we characterize the herbicide indaziflam as a CBI and provide insight into its inhibitory mechanism. Indaziflam-treated seedlings exhibited the CBI-like symptomologies of radial swelling and ectopic lignification. Furthermore, indaziflam inhibited the production of cellulose within ,1 h of treatment and in a dose-dependent manner. Unlike the CBI isoxaben, indaziflam had strong CBI activity in both a monocotylonous plant (Poa annua) and a dicotyledonous plant (Arabidopsis [Arabidopsis thaliana]). Arabidopsis mutants resistant to known CBIs isoxaben or quinoxyphen were not cross resistant to indaziflam, suggesting a different molecular target for indaziflam. To explore this further, we monitored the distribution and mobility of fluorescently labeled CELLULOSE SYNTHASE A (CESA) proteins in living cells of Arabidopsis during indaziflam exposure. Indaziflam caused a reduction in the velocity of YELLOW FLUORESCENT PROTEIN:CESA6 particles at the plasma membrane focal plane compared with controls. Microtubule morphology and motility were not altered after indaziflam treatment. In the hypocotyl expansion zone, indaziflam caused an atypical increase in the density of plasma membrane-localized CESA particles. Interestingly, this was accompanied by a cellulose synthase interacting1-independent reduction in the normal coincidence rate between microtubules and CESA particles. As a CBI, for which there is little evidence of evolved weed resistance, indaziflam represents an important addition to the action mechanisms available for weed management.

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Utilizing R Software Package for Dose-Response Studies: The Concept and Data Analysis

Cited by 434 publications

References 27 publications

Composition and herbicidal effect of Heracleum sosnowskyi essential oil

Composition and herbicidal effect of Heracleum sosnowskyi essential oil

Gene amplification confers glyphosate resistance in Amaranthus palmeri

Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

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