This study addresses the need to implement sustainable alternatives in marine vessels, particularly in vulnerable environments, highlighting the potential of biofuels as a viable option for the transition towards renewable energy. The objective of the research was to evaluate the interest and perception of tourism service providers in Los Cabos, B.C.S., Mexico, regarding the alternative use of biofuels in their vessels. A total of 56 random surveys were applied and validated, and the results were as follows: 92% consider fuel to be their largest operational cost, 84.6% acknowledge that their activities impact the environment, and 80% are aware of biodiesel biofuel. Notably, more than 94% expressed interest in learning how to produce biodiesel and in using it as an alternative in their operations, while 95% believe that the use of biofuels could be a positive differentiating feature for their businesses, contributing to an eco-friendlier service. This introductory assessment is crucial as it highlights the importance of understanding the willingness of key stakeholders before adopting new technologies. It concludes that there is strong interest in exploring and adopting sustainable alternatives, reinforcing the need for further research to provide viable solutions, promoting a circular economy and fostering sustainable tourism in alignment with the Sustainable Development Goals.