UV-aged microplastics induces neurotoxicity by affecting the neurotransmission in larval zebrafish

Xiang, Chongdan; Chen, Haibo; Liu, Xiaolin; Dang, Yao; Li, Xin; Yu, Yunjiang; Li, Bei; Li, Xintong; Sun, Yuhan; Ding, Ping; Hu, Guocheng

doi:10.1016/j.chemosphere.2023.138252

Cited by 24 publications

(9 citation statements)

References 42 publications

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“…Exposure to MNPs can alter gene expression related to neurodevelopment and transmission. MNPs in the brain can inhibit acetylcholinesterase (AChE) and change the neurotransmitters’ levels and the content of neurotransmitters such as dopamine and acetylcholine, leading to neurotoxicity [ 93 ]. For example, exposure to PS-MPs affected the levels of dopamine and acetylcholine in goldfish, as well as the expression of the genes related to neurotransmission [ 94 ].…”

Section: Effects Of Mnps On the Behavior Of Zebrafishmentioning

confidence: 99%

Behavioral Studies of Zebrafish Reveal a New Perspective on the Reproductive Toxicity of Micro- and Nanoplastics

Wu,

Yu,

et al. 2024

Toxics

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The escalating prevalence of microplastics and nanoplastics in aquatic environments is a major challenge affecting the behavior and reproductive health of aquatic organisms while posing potential risks to human health and ecosystems. This review focuses on the neurobehavioral changes and reproductive toxicity of MNPs in zebrafish and their relationships. At the same time, the neurobehavioral changes caused by MNPs were studied, and the synergistic effects of the interaction of these pollutants with other environmental contaminants were explored. In addition, zebrafish, as a model organism, provide valuable insights into the subtle but important effects of MNPs on reproductive behavior, which is critical for understanding reproductive success, suggesting that behavioral changes can serve as an early biomarker of reproductive toxicity. In addition, based on classical endocrine disruptor models and behavioral research methods, the current status of the research on the reproductive toxicity of MNPs in zebrafish was reviewed, which further indicated that the behavioral parameters of zebrafish can be used as an effective and rapid tool to evaluate the reproductive toxicity of MNPs. However, behavioral methods for rapidly assessing the toxicity of MNPs are still an area of exploration. To address limitations and challenges in the current scope of research, this review outlines future research directions with the aim of improving our understanding of the environmental and health impacts of MNPs. This work aims to inform targeted environmental policies and advance public health strategies to address the growing challenge of MNPs pollution.

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Section: Effects Of Mnps On the Behavior Of Zebrafishmentioning

confidence: 99%

Behavioral Studies of Zebrafish Reveal a New Perspective on the Reproductive Toxicity of Micro- and Nanoplastics

Wu,

Yu,

et al. 2024

Toxics

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“…The neurotoxic effects on the GABAergic system are typically investigated in zebrafish by analysing the expression of genes associated with the GABAergic pathway, such as GABA synthesizers [ gad1b ( Cheng et al, 2020 ; Shi et al, 2022 ; Yu et al, 2022 ; Ding et al, 2023 ; Xiang et al, 2023 ) and gad2 ( Ding et al, 2023 )], receptors ( gabra1 ( Shi et al, 2022 ; Liu et al, 2023 ; Ding et al, 2023 ), gabra6a ( Atzei et al, 2021 ), gabra6b ( Liu et al, 2023 ), gabrb3 ( Luo et al, 2023 ), gabrg2 ( Ding et al, 2023 ; Xiang et al, 2023 )), and transporters ( gat1 ( Cheng et al, 2020 ; Ding et al, 2023 ; Xiang et al, 2023 ), gat3 , vgat ). For example, exposure to lead has been shown to increase gad1b and gad2 expression and modulate the expression of gabra1 , gabbr1a , gat1 , gat3 , vgat expression ( Wirbisky et al, 2014 ) ( Supplementary Table S2 ).…”

Section: Neurotransmissionmentioning

confidence: 99%

“…The impact on the dopaminergic system can be investigated by analysing the morphology of dopaminergic neurons ( Cui et al, 2013 ; Yao et al, 2017 ; Jin et al, 2019a ; Wang et al, 2019 ), aided by the availability of zebrafish transgenic lines with GFP-labelled dopaminergic neurons. Analysis of dopamine levels ( Wang et al, 2015 ; Sheng et al, 2016 ; Soares et al, 2017 ; Wang et al, 2019 ; Han et al, 2021 ; Yang et al, 2022 ) and its metabolite, hydroxy-phenyl acetic acid, along with the expression of dopamine transporter ( dat ) genes ( Ji et al, 2021 ; Gyimah et al, 2022 ), dopamine receptors ( drd1b ( Li et al, 2019 ; Tran et al, 2021 ; Hong et al, 2022 ; Liu et al, 2023 ), drd2a ( Cao et al, 2019 ; Gyimah et al, 2022 ; Shi et al, 2022 ; Liu et al, 2023 ; Ding et al, 2023 ; Mahapatra et al, 2023 ; Torres-Ruiz et al, 2023 ; Xiang et al, 2023 ), drd2b [ Shi et al, 2022 ; Liu et al, 2023 ; Torres-Ruiz et al, 2023 )] and developmental genes correlated to dopamine such as manf ( Li et al, 2018 ; Qian et al, 2018 ; Li et al, 2020 ; Zhu et al, 2020 ; Tian et al, 2021 ; Hong et al, 2022 ; Heredia-García et al, 2023 ; Shi et al, 2023 ), otpa , otpb ( Wang et al, 2019 ), wnt1 ( Ho et al, 2013 ), wnt5b ( Ho et al, 2013 ), wnt10b ( Xu et al, 2022a ), wnt4a ( Xu et al, 2022a ), wnt3a , wnt5a ( Wang et al, 2019 ) can be analysed ( Supplementary Table S2 ). Treatment with lead in zebrafish larvae significantly reduced the number of dopaminergic neurons in the midbrain ( Jin et al, 2019b ).…”

Section: Neurotransmissionmentioning

confidence: 99%

Temperature- and chemical-induced neurotoxicity in zebrafish

Toni,

Arena,

Cioni

et al. 2023

Front. Physiol.

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Throughout their lives, humans encounter a plethora of substances capable of inducing neurotoxic effects, including drugs, heavy metals and pesticides. Neurotoxicity manifests when exposure to these chemicals disrupts the normal functioning of the nervous system, and some neurotoxic agents have been linked to neurodegenerative pathologies such as Parkinson’s and Alzheimer’s disease. The growing concern surrounding the neurotoxic impacts of both naturally occurring and man-made toxic substances necessitates the identification of animal models for rapid testing across a wide spectrum of substances and concentrations, and the utilization of tools capable of detecting nervous system alterations spanning from the molecular level up to the behavioural one. Zebrafish (Danio rerio) is gaining prominence in the field of neuroscience due to its versatility. The possibility of analysing all developmental stages (embryo, larva and adult), applying the most common “omics” approaches (transcriptomics, proteomics, lipidomics, etc.) and conducting a wide range of behavioural tests makes zebrafish an excellent model for neurotoxicity studies. This review delves into the main experimental approaches adopted and the main markers analysed in neurotoxicity studies in zebrafish, showing that neurotoxic phenomena can be triggered not only by exposure to chemical substances but also by fluctuations in temperature. The findings presented here serve as a valuable resource for the study of neurotoxicity in zebrafish and define new scenarios in ecotoxicology suggesting that alterations in temperature can synergistically compound the neurotoxic effects of chemical substances, intensifying their detrimental impact on fish populations.

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“…Furthermore, microplastics can act as vectors for harmful chemicals and pathogens, imposing additional threats to human health [5]. Upon uptake, microplastics induce inflammation, neurotoxicity, oxidative stress and changes in the metabolome in eukaryotic organisms [3,6,7]. Polymethylmethacrylate (PMMA) is a synthetic polymer of which its usefulness derives from two properties.…”

Section: Introductionmentioning

confidence: 99%

Uptake and Cellular Effects of Polymethylmethacrylate on Human Cell Lines

Braun,

Seitz

2024

Microplastics

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The usage of plastic and its decomposition products leads to their ubiquitous distribution, resulting in their uptake by all living beings, including humans. Polymethylmethacrylate (PMMA) is known as a biocompatible polymer and is used widely in medicine and dentistry, although recent findings have shown its induction of oxidative stress within cells. Worryingly, hardly any data exist investigating the uptake of PMMA particles by cells, the potential effects of these particles on cells and cell signaling pathways and their contributing factors. We assessed the uptake of PMMA beads via confocal microscopy after their incubation with HEK293, A549 and MRC5 cells. Through cell staining, we localized multiple PMMA beads within the cytosol of cells. No alterations regarding cell growth, cell morphology or cell division were found, implying no short-term toxicity towards human cells. Using a cAMP response element binding protein (CREB)-mediated reporter assay, we assessed whether internalized PMMA nanobeads alter cell signaling pathways after stimulation of the cells. CREB was chosen as a well-described transcription factor involved in various cellular processes. Our data led to the assumption that PMMA nano- and microbeads are internalized via endocytosis and end up in lysosomes within the cell cytosol. We concluded that differences regarding the surface composition of the PMMA nanobeads affect their potential to alter cell signaling. These findings emphasize the key role the surface composition plays regarding microplastics and their risks for human health, whereas the usage of medical-grade PMMA remains safe.

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UV-aged microplastics induces neurotoxicity by affecting the neurotransmission in larval zebrafish

Cited by 24 publications

References 42 publications

Behavioral Studies of Zebrafish Reveal a New Perspective on the Reproductive Toxicity of Micro- and Nanoplastics

Behavioral Studies of Zebrafish Reveal a New Perspective on the Reproductive Toxicity of Micro- and Nanoplastics

Temperature- and chemical-induced neurotoxicity in zebrafish

Uptake and Cellular Effects of Polymethylmethacrylate on Human Cell Lines

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