UV and γ-radiation induced molecular changes for rapid lipid accumulation in Chlorella sorokiniana

Singh, Reema Devi; Sethy, Sunil; Ghosh, Subrata; Srivastava, Ashish Kumar

doi:10.1016/j.biombioe.2022.106493

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…Ionizing radiation has been used as a potent mutagen for Chlamydomonas mutation breeding ( Baek et al., 2016 ; Koo et al., 2017b ). It is also regarded as an oxidative stress inducer that generates ROS via water radiolysis ( Lee et al., 2009 ; Gomes et al., 2017 ; Singh et al., 2022 ). Gamma irradiation at a dose range of 0.5–4 kGy inhibited the growth of Chlorella sorokiniana cells in a dose-dependent manner ( Singh et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…It is also regarded as an oxidative stress inducer that generates ROS via water radiolysis ( Lee et al., 2009 ; Gomes et al., 2017 ; Singh et al., 2022 ). Gamma irradiation at a dose range of 0.5–4 kGy inhibited the growth of Chlorella sorokiniana cells in a dose-dependent manner ( Singh et al., 2022 ). In contrast, this study demonstrated that X- or γ-irradiation at a relatively low dose below 10 Gy substantially increased the growth of C. reinhardtii cells rather than cell death ( Figures 1 , 2 , 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Taken together, the metabolic parameters including growth rate, photosynthetic activity, chlorophyll, protein, starch, and lipid contents seem to be correlated but not in a causal relationship ( Supplementary Figure 4 ). In Chlorella sorokiniana cells, γ-irradiation at a dose range of 0.5–4 kGy decreased growth, chlorophyll content, and photosynthetic efficiency in a dose-dependent manner but increased ROS and lipid accumulation ( Singh et al., 2022 ). The growth stimulatory effects of X- or γ-irradiation with a certain range of relatively low doses in algal cells could be associated with enhanced metabolic activities but not explained by a causal relationship between them.…”

Section: Discussionmentioning

confidence: 99%

“…Upon exposure to γ-rays, Chlamydomonas cells display a dose-dependent change in chlorophyll fluorescence parameters for photosynthetic activity as well as a dose-dependent increase in the formation of reactive oxygen species (ROS) ( Gomes et al., 2017 ). When irradiated with γ-rays of 1 kGy, another green alga, Chlorella sorokiniana , exhibited a substantial decrease in growth, chlorophyll content, and photosynthetic efficiency but a significant increase in the accumulation of neutral lipid triacylglycerol ( Singh et al., 2022 ). These results could be associated with the transcriptional modulation of photosynthesis- and fatty acid biosynthesis-related genes, as well as the accumulation of ROS.…”

Section: Introductionmentioning

confidence: 99%

“…However, when a certain dose range of toxicity or stress factors causes no critical functional damage to cellular metabolism, it contributes to the fitness and sustainable metabolic activities of cells by providing short- or long-term stress memory (priming) through chromatin remodeling and epigenetic regulation ( Kim, 2021 ). Therefore, ionizing radiation may function as a stress memory or priming agent, as well as a damaging agent through ROS accumulation and oxidative stress responses ( Gomes et al., 2017 ; Singh et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Application of ionizing radiation as an elicitor to enhance the growth and metabolic activities in Chlamydomonas reinhardtii

Kim

Dubey²,

Hwangbo³

et al. 2023

Front. Plant Sci.

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Chlamydomonas reinhardtii is a eukaryotic, unicellular photosynthetic organism and a potential algal platform for producing biomass and recombinant proteins for industrial use. Ionizing radiation is a potent genotoxic and mutagenic agent used for algal mutation breeding that induces various DNA damage and repair responses. In this study, however, we explored the counterintuitive bioeffects of ionizing radiation, such as X- and γ-rays, and its potential as an elicitor to facilitate batch or fed-batch cultivation of Chlamydomonas cells. A certain dose range of X- and γ-rays was shown to stimulate the growth and metabolite production of Chlamydomonas cells. X- or γ-irradiation with relatively low doses below 10 Gy substantially increased chlorophyll, protein, starch, and lipid content as well as growth and photosynthetic activity in Chlamydomonas cells without inducing apoptotic cell death. Transcriptome analysis demonstrated the radiation-induced changes in DNA damage response (DDR) and various metabolic pathways with the dose-dependent expression of some DDR genes, such as CrRPA30, CrFEN1, CrKU, CrRAD51, CrOASTL2, CrGST2, and CrRPA70A. However, the overall transcriptomic changes were not causally associated with growth stimulation and/or enhanced metabolic activities. Nevertheless, the radiation-induced growth stimulation was strongly enhanced by repetitive X-irradiation and/or subsequent cultivation with an inorganic carbon source, i.e., NaHCO3, but was significantly inhibited by treatment of ascorbic acid, a scavenger of reactive oxygen species (ROS). The optimal dose range of X-irradiation for growth stimulation differed by genotype and radiation sensitivity. Here, we suggest that ionizing radiation within a certain dose range determined by genotype-dependent radiation sensitivity could induce growth stimulation and enhance metabolic activities, including photosynthesis, chlorophyll, protein, starch, and lipid synthesis in Chlamydomonas cells via ROS signaling. The counterintuitive benefits of a genotoxic and abiotic stress factor, i.e., ionizing radiation, in a unicellular algal organism, i.e., Chlamydomonas, may be explained by epigenetic stress memory or priming effects associated with ROS-mediated metabolic remodeling.

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